Effect of Clausena harmandiana root extract, Garcinia mangostana Linn rind extract and whole Andrographis paniculata extracts on growth of Pythium insidiosum
Issued Date
2016
Resource Type
Language
eng
Rights
Mahidol University
Rights Holder(s)
Prince of Songkla University
Suggested Citation
Nujarin Jongruja, Tassanee Lohnoo, Wanta Yingyong, Yothin Kumsang, Thidarat Rujirawat, Tassanee Lerksuthirat, Kovit Pattanapanyasat (2016). Effect of Clausena harmandiana root extract, Garcinia mangostana Linn rind extract and whole Andrographis paniculata extracts on growth of Pythium insidiosum. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/61245
Title
Effect of Clausena harmandiana root extract, Garcinia mangostana Linn rind extract and whole Andrographis paniculata extracts on growth of Pythium insidiosum
Other Contributor(s)
King Mongkut’s University of Technology Thonburi. Faculty of Science. Department of Microbiology
Mahidol University. Faculty of Medicine, Ramathibodi Hospital. Research Center
Mahidol University. Faculty of Medicine, Ramathibodi Hospital. Department of Radiology
Mahidol University. Faculty of Science. Multidisciplinary Unit
Mahidol University. Faculty of Medicine, Siriraj Hospital
Mahidol University. Faculty of Medicine, Ramathibodi Hospital. Research Center
Mahidol University. Faculty of Medicine, Ramathibodi Hospital. Department of Radiology
Mahidol University. Faculty of Science. Multidisciplinary Unit
Mahidol University. Faculty of Medicine, Siriraj Hospital
Abstract
Pythium insidiosum is categorized into a group of oomycetes, that are filamentous eukaryotic microorganisms. P. insidiosum is
the only species reported e ie a human and animal pathogen. The disease caused by the pathogen is called pythiosis. Pythiosis is
one of the life-threatening infectious diseases, because it may lead to the loss of infected tissues, organs or mortality.
Although the disease severity is relatively high, effective and suitable medicines remain unavailable. This study aimed to
evaluate the effective inhibitory activity of extracts from Garcinia mangostana Linn fruit rind and Andrographis paniculata
against various isolates of P. insidiosum and their inhibitory activity compared to the root extract of Clausena harmandiana.
The crude extracts were tested for antimicrobial aaeahaey against twenty clinical and environmental isolates of P. insidiosum
by determination of their minimum inhibitory concentration (MIC) values by agar well diffusion and disc diffusion assays.
The present study indicated that extracts from the garcinia fruit rind had inhibitory activity against clinical isolates of P.
insidiosum. Every isolate of P. insidiosum was inhibited by 2 mg/ml of the mangosteen fruit rind extract, whereas whole
Andrographis paniculata and Clausena harmandiana root extracts exhibited inhibitory activity against all isolates at 5
mg/ml and 10 mg/ml, respectively. Results from the agar well diffusion and disc diffusion assays also indicated that the
extracts from Garcinia mangostana Linn fruit rind might be effective and were promising for future studies as one of the
medicines for treating pythiosis. The ethanolic extract of the mangosteen rind had a slightly higher phenolic content than the
aqueous extract. Therefore, the ethanolic extract from G. mangostana Linn fruit rind was considered to be appropriate for
further studies for drug discovery and development for treatment of pythiosis disease.
Description
The 5th International Biochemistry and Molecular Biology Conference 2016 (BMB2016). Departments of Molecular Biology and Bioinformatics and the Department of Biochemistry, Faculty of Science, Prince of Songkla University. May 26 to 27, 2016. p.439-442.