Immunomics analysis of excretory-secretory antigens from 3rd stage larva gnathostoma spinigerum for development of immunodiagnosis
Issued Date
2014
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Supaporn Nuamtanong, สุภาพร น่วมทะนง, Paron Dekumyoy, พารณ ดีคำย้อย, Onrapak Reamtong, อรภัค เลี่ยมทอง, Poom Adisakwattana, ภูมิ อดิศักดิ์วัฒนา, Mahidol University. Faculty of Tropical Medicine. Department of Molecular Tropical Medicine and Genetics (2014). Immunomics analysis of excretory-secretory antigens from 3rd stage larva gnathostoma spinigerum for development of immunodiagnosis. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/63164
Title
Immunomics analysis of excretory-secretory antigens from 3rd stage larva gnathostoma spinigerum for development of immunodiagnosis
Other Contributor(s)
Abstract
The parasite Gnathostoma spinigerum is a causative agent of
gnathostomiasis and can be found in several countries. Infection leads to
permanent organ damage or death, therefore rapid and reliable diagnosis
is necessary and a major priority for effective treatment. In our investigation we
determined novel target proteins from excretory-secretory (ES) antigens of 3rd stage G.
spinigerum larva using immunomics and mass spectrometry approaches. We prepared
ES antigen of G. spinigerum for protein profiling analysis by 2D-gel electrophoresis
and subsequently transferred it onto PVDF membrane. The membranes were reacted
with G. spinigerum infected or other helminthic infected sera. Results show that
approximately seven spots at the molecular size of 12, 20, and 50 kDa represented
G. spinigerum infection. Next, these protein spots are going to be analyzed by mass
spectrometry and available databases will be searched for annotation. Identifying the
type of protein and/or tryptic peptide sequences will be useful for the development of
future immunodiagnosis for gnathostomiasis.
Description
Joint International Tropical Medicine Meeting 2014: 3D perspectives on tropical medicine: drivers, diversity and determination the 8th seminar on food-and water-borne parasitic zoonoses: 2-4 December 2014: Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2014. p. 247.