Evaluation of T cell responses to naturally processed variant SARS-CoV-2 spike antigens in individuals following infection or vaccination
Issued Date
2023-05-30
Resource Type
eISSN
22111247
Scopus ID
2-s2.0-85154056381
Journal Title
Cell Reports
Volume
42
Issue
5
Rights Holder(s)
SCOPUS
Bibliographic Citation
Cell Reports Vol.42 No.5 (2023)
Suggested Citation
Yin Z., Chen J.L., Lu Y., Wang B., Godfrey L., Mentzer A.J., Yao X., Liu G., Wellington D., Zhao Y., Wing P.A.C., Dejnirattisa W., Supasa P., Liu C., Hublitz P., Beveridge R., Waugh C., Clark S.A., Clark K., Sopp P., Rostron T., Mongkolsapaya J., Screaton G.R., Ogg G., Ewer K., Pollard A.J., Gilbert S., Knight J.C., Lambe T., Smith G.L., Dong T., Peng Y. Evaluation of T cell responses to naturally processed variant SARS-CoV-2 spike antigens in individuals following infection or vaccination. Cell Reports Vol.42 No.5 (2023). doi:10.1016/j.celrep.2023.112470 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/82746
Title
Evaluation of T cell responses to naturally processed variant SARS-CoV-2 spike antigens in individuals following infection or vaccination
Author(s)
Yin Z.
Chen J.L.
Lu Y.
Wang B.
Godfrey L.
Mentzer A.J.
Yao X.
Liu G.
Wellington D.
Zhao Y.
Wing P.A.C.
Dejnirattisa W.
Supasa P.
Liu C.
Hublitz P.
Beveridge R.
Waugh C.
Clark S.A.
Clark K.
Sopp P.
Rostron T.
Mongkolsapaya J.
Screaton G.R.
Ogg G.
Ewer K.
Pollard A.J.
Gilbert S.
Knight J.C.
Lambe T.
Smith G.L.
Dong T.
Peng Y.
Chen J.L.
Lu Y.
Wang B.
Godfrey L.
Mentzer A.J.
Yao X.
Liu G.
Wellington D.
Zhao Y.
Wing P.A.C.
Dejnirattisa W.
Supasa P.
Liu C.
Hublitz P.
Beveridge R.
Waugh C.
Clark S.A.
Clark K.
Sopp P.
Rostron T.
Mongkolsapaya J.
Screaton G.R.
Ogg G.
Ewer K.
Pollard A.J.
Gilbert S.
Knight J.C.
Lambe T.
Smith G.L.
Dong T.
Peng Y.
Author's Affiliation
Siriraj Hospital
NIHR Oxford Biomedical Research Centre
Beijing YouAn Hospital, Capital Medical University
The Wellcome Centre for Human Genetics
University of Cambridge
University of Oxford
Nuffield Department of Medicine
University of Oxford Medical Sciences Division
MRC Weatherall Institute of Molecular Medicine
NIHR Oxford Biomedical Research Centre
Beijing YouAn Hospital, Capital Medical University
The Wellcome Centre for Human Genetics
University of Cambridge
University of Oxford
Nuffield Department of Medicine
University of Oxford Medical Sciences Division
MRC Weatherall Institute of Molecular Medicine
Other Contributor(s)
Abstract
Most existing studies characterizing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific T cell responses are peptide based. This does not allow evaluation of whether tested peptides are processed and presented canonically. In this study, we use recombinant vaccinia virus (rVACV)-mediated expression of SARS-CoV-2 spike protein and SARS-CoV-2 infection of angiotensin-converting enzyme (ACE)-2-transduced B cell lines to evaluate overall T cell responses in a small cohort of recovered COVID-19 patients and uninfected donors vaccinated with ChAdOx1 nCoV-19. We show that rVACV expression of SARS-CoV-2 antigen can be used as an alternative to SARS-CoV-2 infection to evaluate T cell responses to naturally processed spike antigens. In addition, the rVACV system can be used to evaluate the cross-reactivity of memory T cells to variants of concern (VOCs) and to identify epitope escape mutants. Finally, our data show that both natural infection and vaccination could induce multi-functional T cell responses with overall T cell responses remaining despite the identification of escape mutations.