Elicitation increased chromones accumulation and anti-inflammatory activity in Harrisonia perforata (Blanco) Merr. cell cultures
3
Issued Date
2025-08-01
Resource Type
ISSN
02546299
Scopus ID
2-s2.0-105006648901
Journal Title
South African Journal of Botany
Volume
183
Start Page
187
End Page
192
Rights Holder(s)
SCOPUS
Bibliographic Citation
South African Journal of Botany Vol.183 (2025) , 187-192
Suggested Citation
Boonsnongcheep P., Petchprowsang W., Akkakraisee W., Pichetpongtorn P., Suksamrarn A., Chantorn S., Bunsupa S., Kitisripanya T. Elicitation increased chromones accumulation and anti-inflammatory activity in Harrisonia perforata (Blanco) Merr. cell cultures. South African Journal of Botany Vol.183 (2025) , 187-192. 192. doi:10.1016/j.sajb.2025.05.031 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/110547
Title
Elicitation increased chromones accumulation and anti-inflammatory activity in Harrisonia perforata (Blanco) Merr. cell cultures
Corresponding Author(s)
Other Contributor(s)
Abstract
Harrisonia perforata is an essential ingredient in Thai traditional medicine's Ha-Rak herbal preparation, traditionally requiring equal proportions of roots from five plants, raising sustainability concerns regarding sourcing from natural habitats. This study aimed to establish an in vitro callus and cell suspension culture system of H. perforata for sustainable production of two bioactive chromones—O-methylalloptaeroxylin and perforamone D—and to evaluate their anti-inflammatory potential. Optimal callus induction and cell suspension growth were achieved using Murashige and Skoog (MS) medium supplemented with 1.0 mg/L TDZ and 0.5 mg/L NAA. Treatment of cell suspension cultures with elicitors significantly enhances chromone accumulation. Maximum O-methylalloptaeroxylin (60.93 ± 0.62 µg/g DW; 19.2-fold increase) was recorded after elicitation with 10 µM salicylic acid (SA) for 3 days, while perforamone D reached its highest level (18.74 ± 1.17 µg/g DW; 1.7-fold increase) following treatment with 100 mg/L yeast extract (YE) for 3 days. Anti-inflammatory activity, assessed by nitric oxide inhibition in LPS-stimulated RAW 264.7 macrophages, correlated significantly with perforamone D concentration (r = 0.387, p < 0.01). The established in vitro culture system demonstrates the potential for sustainable production of these valuable compounds. However, further optimization is required for practical large-scale applications.