Somatic embryogenesis of (Saccharum officinarum var Hawaii) and isozyme comparison of regenerated plant by isoelectricfocusing
1
Issued Date
2024
Copyright Date
1991
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xiii, 75 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Environmental Biology))--Mahidol University, 1991
Suggested Citation
Laksana Kantama Somatic embryogenesis of (Saccharum officinarum var Hawaii) and isozyme comparison of regenerated plant by isoelectricfocusing. Thesis (M.Sc. (Environmental Biology))--Mahidol University, 1991. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/100176
Title
Somatic embryogenesis of (Saccharum officinarum var Hawaii) and isozyme comparison of regenerated plant by isoelectricfocusing
Alternative Title(s)
การเกิดคัพภะจากาโซมาติคเซลของอ้อย (Saccharum officinarum var. Hawaii) และการเปรียบเทียบไอโซไซม์ของพืชที่ได้จากการเพาะเลี้ยงโดยวิธีไอโซอีเลคทริคโฟกัสซิ่ง
Author(s)
Abstract
Callus induction was established from the young leaves of sugarcane (Saccharum officinarum var. Hawaii) on modified Murashige and Skoog (MS) medium supplemented with various concentrations of 2,4-D and 10% coconut water. The medium supplemented with 3 mg/l 2,4-D was the optimum on callus induction for the explants derived from 1.5-4 cm above the youngest node. While the medium supplemented with 3 mg/l 2,4-D and 10% coconut water produced green pin headed structures on the explant derived from 5-6 cm above the node. The calli were proliferated and maintained on modified MS medium supplemented with 0.5 mg/l 2,4-D. Embryogenic induction was achieved on modified MS medium with or without 0.5 mg/l 2,4-D and 10% coconut water. The embryogenic calli were transferred to modified MS medium containing 1.0% or 1.5% saccharosee for embrygenic development. In order to promote shoot elongation and pre-embryogenic callus recycling, the embryoid cluster was transferred to modified MS medium supplemented with 3 mg/l 2,4-D and 10% coconut water. Then the plantlets were transferred to modified MS medium supplemented with 1 mg/l IAA for for root development. The plantlets were transferred to pots with vermiculite and watered with complete fertilized for a month before establishing in soil. The regenerated plants could be derived in 3 groups by morphological comparisons. From isozyme isoelectricfocusing, there are 2 samples of regenerated plants showed two bands missing in esterase zymogram as compared to the donors.
Description
Environmental Biology (Mahidol University 1991)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Environmental Biology
Degree Grantor(s)
Mahidol University