The effect of stannous fluoride desensitizing toothpaste on immediate dentin sealing ability of universal adhesive in vitro

Abstract

Background/purpose: The stannous fluoride pretreatment of dentin may compromise the bond formation of the dentinal adhesive system. This study aimed to determine the effect of stannous fluoride (SnF2) desensitizing toothpaste on immediate dentin sealing ability (IDS) of universal adhesive in human intact dentin using dentin permeability test and scanning electron microscopy (SEM) and energy dispersive x-ray spectroscopy (EDS). Materials and methods: Sixty sound dentin specimens were prepared from 60 extracted intact third molars, then treated with 17 % EDTA for 60 s to simulate hypersensitive dentin, and randomly divided into 2 series. Series 1 (dentin permeability test; n = 40) had 4 different treatment groups: (1) universal adhesive (self-etch mode), (2) universal adhesive (total-etch mode), (3) SnF2 pretreatment + universal adhesive (self-etch), (4) SnF2 pretreatment + universal adhesive (total-etch). The IDS of each group was the percentage decrease of hydraulic conductance of dentin after bonding. Series 2 (n = 20) was SEM-EDS analysis of different treated dentin before bonding. The data were analyzed using 2-way ANOVA and Tukey test. Results: In groups of no SnF2 pretreatment, the IDS of universal adhesive were 79.53 ± 20.71 and 77.34 ± 18.14 % for self-etch and total-etch mode, respectively. In groups of SnF2 pretreatment, the corresponding values were 71.54 ± 15.56 and 69.56 ± 9.92 %, respectively. Although SnF2 pretreated-dentin had the remarkable signals of silicon and tin, there were no significant reductions in the IDS of universal adhesive (P < 0.05). Conclusion: SnF2 desensitizing toothpaste has no adverse effect on the immediate dentin sealing ability of universal adhesive.