Comprehensive analysis of miRNA profiling in Schistosoma mekongi across life cycle stages
Issued Date
2024-12-01
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-85183385543
Journal Title
Scientific Reports
Volume
14
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports Vol.14 No.1 (2024)
Suggested Citation
Sivapornnukul P., Khamwut A., Chanchaem P., Chusongsang P., Chusongsang Y., Poodeepiyasawat P., Limpanont Y., Reamtong O., Payungporn S. Comprehensive analysis of miRNA profiling in Schistosoma mekongi across life cycle stages. Scientific Reports Vol.14 No.1 (2024). doi:10.1038/s41598-024-52835-5 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/95837
Title
Comprehensive analysis of miRNA profiling in Schistosoma mekongi across life cycle stages
Corresponding Author(s)
Other Contributor(s)
Abstract
Schistosoma mekongi, a significant schistosome parasite, has various life stages, including egg, cercaria, female, and male, that play crucial roles in the complex life cycle. This study aimed to explore the microRNA (miRNA) profiles across these developmental stages to understand their potential functions and evolutionary significance, which have not been studied. Pre-processed sequencing reads of small RNA (sRNA) were obtained, and annotations were performed against the S. japonicum reference miRNA database. Results indicated marked variations in miRNA profiles across different life stages, with notable similarities observed between female and male S. mekongi. Principal Coordinate Analysis (PCoA) and unsupervised clustering revealed distinct miRNA signatures for each stage. Gene ontology (GO) analysis unveiled the potential roles of these miRNAs in various biological processes. The differential expression of specific miRNAs was prominent across stages, suggesting their involvement in crucial developmental processes. Furthermore, orthologous miRNA analysis against various worm species revealed distinct presence–absence patterns, providing insights into the evolutionary relationships of these miRNAs. In conclusion, this comprehensive investigation into the miRNA profiles of S. mekongi offers valuable insights into the functional and evolutionary aspects of miRNAs in schistosome biology.