Generation of human induced pluripotent stem cell (DMSCi001-A) line from hematopoietic stem cells of a healthy female donor
Issued Date
2024-12-01
Resource Type
ISSN
18735061
eISSN
18767753
Scopus ID
2-s2.0-85208066531
Journal Title
Stem Cell Research
Volume
81
Rights Holder(s)
SCOPUS
Bibliographic Citation
Stem Cell Research Vol.81 (2024)
Suggested Citation
Wongkidakarn S., Yodtup C., Jantapalaboon D., Phairoh P., Suparak S., Dhepakson P., Tubsuwan A., Bumroongthai K. Generation of human induced pluripotent stem cell (DMSCi001-A) line from hematopoietic stem cells of a healthy female donor. Stem Cell Research Vol.81 (2024). doi:10.1016/j.scr.2024.103605 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/101956
Title
Generation of human induced pluripotent stem cell (DMSCi001-A) line from hematopoietic stem cells of a healthy female donor
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Abstract
Hematopoietic stem cell isolated from a healthy 39-year-old woman were successfully reprogrammed and transformed into induced pluripotent stem cell (iPSCs) by using the integration-free episomal vector included OCT3/4/shp53, Sox2/KLF4, L-MYC/LIN28 and EBNA-1 reprogramming factors. The transformed iPSC lines were cultured and expanded under feeder-free condition. They demonstrated the normal karyotype, expressed pluripotency markers and differentiated into cells derived from the three germ layers. These iPSCs are capable of differentiating into numerous cell subtypes for the purposes of drug discovery and mechanism investigation.