Development of rapid detection of vibrio cholera in immunomagnetic enriched samples by mab-based dot-blot elisa
Issued Date
2002
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Yuvadee Mahakunkijcharoen, ยุวดี มหาคุณกิจเจริญ, Pramuan Tapchaisri, Manas Chongsa-nguan, มนัส จงสงวน, Wanpen Chaicumpa, วันเพ็ญ ชัยคำภา (2002). Development of rapid detection of vibrio cholera in immunomagnetic enriched samples by mab-based dot-blot elisa. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/63373
Title
Development of rapid detection of vibrio cholera in immunomagnetic enriched samples by mab-based dot-blot elisa
Abstract
Ab-reacted beads have been produced by reacting goat anti-rabbit immunoglobulin G (IgG) coated superparamagnetic beads (BioMag) with 100 mg/ml of Vibrio cholerae (Vc)-specific rabbit Igs. The beads were used to capture Vc or its antigensin food samples. The antigens (Ag) on the Ag-captured beads were then detected by MAb-based dot-blot ELISA. This immunomagnetic enrichment (IME) was able to detect V. cholerae suspended in the food samples as low as 106 cfu/ml. By using this IME/MAb-based dot-blot ELISA we could detect V. cholerae in 100% (all 50 samples) of artificially seeded food samples, whereas the conventional culture method showed only 32% positive. Enrichment of the bacteria by IME prior to conventional culturing increased percent positivity from 20.59% (7 of 34 samples) to 52.94% (18 of 34 samples) or about 2.5 times. The method would be therefore useful for large scale screening, especially in the endemic areas because the method is simple, rapid and gives not much waste products as those derived from conventional culture method.
Description
Joint International Tropical Medicine 2002: the 8th Chamlong-Tranakchit Harinsuta Lecture 20-22 November 2002 Montien Riverside Hotel, Bangkok, Thailand: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2002. p. 199.