Exploring the mechanisms of clozapine-induced blood–brain barrier dysfunction using untargeted metabolomics and cellular metabolism analysis
Issued Date
2023-09-01
Resource Type
ISSN
13826689
eISSN
18727077
Scopus ID
2-s2.0-85165147011
Pubmed ID
37451530
Journal Title
Environmental Toxicology and Pharmacology
Volume
102
Rights Holder(s)
SCOPUS
Bibliographic Citation
Environmental Toxicology and Pharmacology Vol.102 (2023)
Suggested Citation
Ngamratanapaiboon S., Srikornvit N., Hongthawonsiri P., Pornchokchai K., Wongpitoonmanachai S., Pholkla P., Mo J., Yambangyang P., Ayutthaya W.D.N. Exploring the mechanisms of clozapine-induced blood–brain barrier dysfunction using untargeted metabolomics and cellular metabolism analysis. Environmental Toxicology and Pharmacology Vol.102 (2023). doi:10.1016/j.etap.2023.104219 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/88112
Title
Exploring the mechanisms of clozapine-induced blood–brain barrier dysfunction using untargeted metabolomics and cellular metabolism analysis
Author's Affiliation
Other Contributor(s)
Abstract
Brain microvascular endothelial cells (BMVECs) from the blood– brain barrier form a highly selective membrane that protects the brain from circulating blood and maintains a stable microenvironment for the central nervous system. BMVEC dysfunction has been implicated in a variety of neurological and psychiatric disorders. Clozapine, a widely used antipsychotics, has been demonstrated to alter the permeability of BMVECs, but the underlying mechanisms of this effect are not fully understood. In this study, we investigated the effects of clozapine in BMVECs using untargeted metabolomics analysis. Our results illustrated that treatment with clozapine led to significant changes in the metabolic profile of BMVECs, including alterations in amino acid and energy metabolism. These findings suggest that clozapine affects BMVEC permeability through its effects on cellular metabolism. Our study could inform the development of more targeted and effective treatments for understanding the relationships among clozapine, cellular metabolism, and BMVECs in more detail.