Proteomic and Allergenomic Profiling of Banana (Musa spp.): Identification of Potential Novel Allergens in Thai Adult Banana Allergy Cohort
Issued Date
2025-01-01
Resource Type
eISSN
11786965
Scopus ID
2-s2.0-105022499942
Journal Title
Journal of Asthma and Allergy
Volume
18
Start Page
1641
End Page
1650
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Asthma and Allergy Vol.18 (2025) , 1641-1650
Suggested Citation
Sangsuwan P., Reamtong O., Indrawattana N., Saelim N., Leeanan R., Srisai T., Wongsa C., Thongngarm T., Tsui S.K.W., Sompornrattanaphan M., Tungtrongchitr A. Proteomic and Allergenomic Profiling of Banana (Musa spp.): Identification of Potential Novel Allergens in Thai Adult Banana Allergy Cohort. Journal of Asthma and Allergy Vol.18 (2025) , 1641-1650. 1650. doi:10.2147/JAA.S554945 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/113311
Title
Proteomic and Allergenomic Profiling of Banana (Musa spp.): Identification of Potential Novel Allergens in Thai Adult Banana Allergy Cohort
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Abstract
Purpose: Bananas are one of the most widely consumed fruits globally, particularly in Thailand. However, banana fruits have been recognized as allergenic, with the WHO/IUIS currently listing six identified banana allergens. This study investigates the proteome and allergenome of crude protein extracts from Musa ‘Kluai Hom Thong’ bananas. Patients and Methods: Proteins were separated using two-dimensional gel electrophoresis (2DE) and characterized through 2DE-IgE immunoblotting with sera from 20 banana-allergic patients. Reactive protein spots were analyzed using LC-MS/MS, and proteins were identified via database searches against UniProt-Musaceae. Results: A total of 559 proteins were identified, with 35 reactive protein spots detected across the sera samples, corresponding to 19 distinct proteins. Notably, none of these proteins have been previously reported as banana allergens, categorizing them as potential novel allergens. Among these, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and pathogenesis-related (PR) proteins are notable, as they are major allergens in other fruits and organisms. Conclusion: Nineteen IgE-reactive proteins were identified from Musa ‘Kluai Hom Thong’, with GAPDH and PR proteins proposed as candidate novel allergens associated with systemic reactions and cross-reactivity. Future multi-center studies incorporating functional assays are needed to validate their clinical relevance and diagnostic potential.