A highly sensitive and selective rhodamine-semicarbazide based fluorescent sensor for Cu<sup>2+</sup> detection in real water samples and fluorescence bioimaging in HepG2 cells
Issued Date
2024-04-01
Resource Type
ISSN
00399140
Scopus ID
2-s2.0-85179619604
Pubmed ID
38091746
Journal Title
Talanta
Volume
270
Rights Holder(s)
SCOPUS
Bibliographic Citation
Talanta Vol.270 (2024)
Suggested Citation
Wechakorn K., Eiamprasert U., Masoongnoen J., Tantipanjaporn A., Surawatanawong P., Kanjanasirirat P., Pewkliang Y., Borwornpinyo S., Kongsaeree P., Pitsanuwong C. A highly sensitive and selective rhodamine-semicarbazide based fluorescent sensor for Cu<sup>2+</sup> detection in real water samples and fluorescence bioimaging in HepG2 cells. Talanta Vol.270 (2024). doi:10.1016/j.talanta.2023.125530 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/95980
Title
A highly sensitive and selective rhodamine-semicarbazide based fluorescent sensor for Cu<sup>2+</sup> detection in real water samples and fluorescence bioimaging in HepG2 cells
Corresponding Author(s)
Other Contributor(s)
Abstract
A colorimetric and fluorescent sensor, selective for Cu2+ ions, was synthesized in two steps using a rhodamine-based compound attached to the semicarbazide-picolylamine moiety (RBP). Spectroscopic measurements, including UV–Vis absorption and fluorescence emission, were conducted in the semi-aqueous medium containing acetonitrile/4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, denoted as MeCN/HEPES buffer (2:8, v/v, pH 7.0). The sensor exhibited high selectivity towards Cu2+ ions compared to other cations and demonstrated remarkable sensitivity towards Cu2+ ions, with a limit of detection at the nanomolar level. The calculated transitions indicated a 1:1 stoichiometric binding of RBP to Cu2+ ions based on a 4-coordination mode involving additional chelation in the semi-aqueous medium. The sensing mechanism for the detection of Cu2+ ions was investigated using high-resolution mass spectroscopy. The sensor could be employed as a real-time chemosensor for monitoring Cu2+ ions. Furthermore, the sensor has the potential for utilization in the detection of Cu2+ ions in actual water samples with the high precision and accuracy, as indicated by the small relative standard derivation values. The 50th percentile cytotoxicity concentration of RBP was found to be 22.92 μM. Additionally, the fluorescence bioimaging capability of RBP was demonstrated for the detection of Cu2+ ions in human hepatocellular carcinoma (HepG2) cells.