Enhancing efficiency in detection of COVID-19 through AI-driven colorimetric isothermal detection with multiplex primers
Issued Date
2024-10-01
Resource Type
ISSN
07328893
eISSN
18790070
Scopus ID
2-s2.0-85200221791
Journal Title
Diagnostic Microbiology and Infectious Disease
Volume
110
Issue
2
Rights Holder(s)
SCOPUS
Bibliographic Citation
Diagnostic Microbiology and Infectious Disease Vol.110 No.2 (2024)
Suggested Citation
Jaroenram W., Teerapittayanon S., Suvannakad R., Pengpanich S., Kampeera J., Arunrut N., Dangtip S., Sirithammajak S., Tondee B., Khumwan P., Japakasetr S., Leaungwutiwong P., Chatnuntawech I., Kiatpathomchai W. Enhancing efficiency in detection of COVID-19 through AI-driven colorimetric isothermal detection with multiplex primers. Diagnostic Microbiology and Infectious Disease Vol.110 No.2 (2024). doi:10.1016/j.diagmicrobio.2024.116446 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/100396
Title
Enhancing efficiency in detection of COVID-19 through AI-driven colorimetric isothermal detection with multiplex primers
Corresponding Author(s)
Other Contributor(s)
Abstract
COVID-19 has afflicted millions of lives worldwide. Although there are many rapid methods to detect it based on colorimetric loop-mediated isothermal amplification, there remains room for improvement. This study aims to 1) integrate multiple primers into a singleplex assay to enhance the diagnostic sensitivity, and 2) utilize a high-throughput smartphone-operatable AI-driven color reading tool to enable a rapid result analysis. This setup can improve the sensitivity by 10–100 times and can analyze approximately 6700 samples per minute. The assay is simpler than RT-qPCR, with a turnaround time of less than 75 min. It can detect various types of SARS-CoV-2 by targeting 3 genes, increasing the likelihood that it will remain effective even if the virus undergoes mutations in any single target gene. In summary, it affords potential for adaptation to detection of new/re-emerging diseases with the visual readout for maximum assay simplicity and AI-operated mode for large-scale testing.