Further studies on the ingluence of thiamine deficiency on DMN-demethylase system in the arts

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dc.contributor.advisorMathuros Ruchirawat
dc.contributor.authorChuthaporn Akathos
dc.date.accessioned2025-02-03T07:43:16Z
dc.date.available2025-02-03T07:43:16Z
dc.date.copyright1988
dc.date.created2025
dc.date.issued1988
dc.descriptionToxicology (Mahidol University 1988)
dc.description.abstractThe DMN-demethylase system in rat kidney microsomes consisted of at least two isozymes designated as DMN-demethylase I and DMN-demethylase II which operated at low concethration (4-50 mM) and high concentration of DMN (above 50 mM) respectively. The optimum substrate concentrations determined were 4 mM for DMN-demethylase I and 200 mM for DMN-demethylase II. The activity of DMN-demethylase I and DMN-demethylase II of kidney microsomes were only 11% and 16% of the liver enzymes, respectively. In the kidney enzyme system, phenobarbital pretreatment had no effect on DMN-demethylase I and DMN-demethylase II activities. While 3-MC pretreatment incresed both DMN-demethlase I (37%) and DMN-demethylase II (30%). Thiamine deficiency enhanced both the kidney DMN-demethylase I and DMN-demethylase II activities. This effect is different from that of the liver system in which thiamine deficiency markedly enhanced the activity of DMN-demethylase II without a significant effect on DMN-demethylase I. Phenobarbital pretreatment in thiamine deficient rats produced no effects on kidney DMN-demethylase activity as also observed in the controls. For 3-MC pretreatment, only DMN-demethylase II was 22% decreased. Thiamine hydrochloride supplementation could reverse the enhanced effect of thiamine deficiency and this effect was not mediated through the level of TPP in the microsomal enzyme system.
dc.format.extentx, 106 leaves : ill.
dc.format.mimetypeapplication/pdf
dc.identifier.citationThesis (M.Sc. (Toxicology))--Mahidol University, 1988
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/103266
dc.language.isoeng
dc.publisherMahidol University. Mahidol University Library and Knowledge Center
dc.rightsผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
dc.rights.holderMahidol University
dc.subjectDimethylnitrosamine -- Metabolism
dc.subjectEnzyme Induction
dc.subjectEnzyme Inhibitors
dc.subjectThiamine deficiency
dc.titleFurther studies on the ingluence of thiamine deficiency on DMN-demethylase system in the arts
dc.title.alternativeการศึกษาระบบเอ็นไซม์ DMN-demethylase ในตับไตและอิทธิพลของการขาดวิตามินบี
dc.typeMaster Thesis
dcterms.accessRightsopen access
mods.location.urlhttp://mulinet11.li.mahidol.ac.th/e-thesis/scan/10749123.pdf
thesis.degree.departmentFaculty of Science
thesis.degree.disciplineToxicology
thesis.degree.grantorMahidol University
thesis.degree.levelMaster's degree
thesis.degree.nameMaster of Science

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