The Interplay of Protein Hydrolysis and Ammonia in the Stability of Hevea Rubber Latex during Storage
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Issued Date
2023-12-01
Resource Type
eISSN
20734360
Scopus ID
2-s2.0-85180726159
Journal Title
Polymers
Volume
15
Issue
24
Rights Holder(s)
SCOPUS
Bibliographic Citation
Polymers Vol.15 No.24 (2023)
Suggested Citation
Payungwong N., Sakdapipanich J., Wu J., Ho C.C. The Interplay of Protein Hydrolysis and Ammonia in the Stability of Hevea Rubber Latex during Storage. Polymers Vol.15 No.24 (2023). doi:10.3390/polym15244636 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/95764
Title
The Interplay of Protein Hydrolysis and Ammonia in the Stability of Hevea Rubber Latex during Storage
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Corresponding Author(s)
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Abstract
Natural rubber (NR) latex derived from Hevea brasiliensis is a complex colloid comprising mainly rubber hydrocarbons (latex particles) and a multitude of minor non-rubber constituents such as non-rubber particles, proteins, lipids, carbohydrates, and soluble organic and inorganic substances. NR latex is susceptible to enzymatic attack after it leaves the trees. It is usually preserved with ammonia and, to a lesser extent, with other preservatives to enhance its colloidal stability during storage. Despite numerous studies in the literature on the influence of rubber proteins on NR latex stability, issues regarding the effect of protein hydrolysis in the presence of ammonia on latex stability during storage are still far from resolved. The present work aims to elucidate the interplay between protein hydrolysis and ammoniation in NR latex stability. Both high- and low-ammonia (with a secondary preservative) NR latexes were used to monitor the changes in their protein compositions during storage. High-ammonia (FNR-A) latex preserved with 0.6% (v/v) ammonia, a low 0.1% ammonia/TMTD/ZnO (FNR-TZ) latex, and a deproteinized NR (PDNR) latex were labeled with fluorescence agents and observed using confocal laser scanning microscopy to determine their protein composition. Protein hydrolysis was confirmed via sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). The results revealed that protein hydrolysis increased with the storage duration. The change in protein composition accompanying hydrolysis also allows the spatial distribution of allergenic proteins to be estimated in the latex. Concurrently, the latex stability increased with the storage duration, as measured by the latex’s mechanical stability time (MST) and the zeta potential of the latex particles. As monitored by AFM, the surface roughness of the NR latex film increased markedly during extended storage compared with that of the DPNR latex, which remained smooth. These results underscore the pivotal role of ammonia in bolstering NR latex stability brought on by protein hydrolysis, which greatly impacts latex film’s formation behavior. NR latex stability underpins the quality of latex-dipped goods during manufacturing, particularly those for medical gloves.