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Author: Chaitip Wanichanon

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Now showing 1 - 6 of 6
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    Immunohistochemical detection of GnRH-like peptides in the neural ganglia and testis of Haliotis asinina
    (2014-02-01) Parinyaporn Nuurai; Jeerawan Primphon; Tawanchay Seangcharoen; Yotsawan Tinikul; Chaitip Wanichanon; Prasert Sobhon; Burapha University; Mahidol University
    Gonadotropin releasing hormone (GnRH) is a peptide that is conserved in both vertebrate and invertebrate species. In this study, we have demonstrated the distribution pattern of two isoforms of GnRH-like peptides in the neural ganglia and testis... of the pleuropedal ganglia. In the testis, only octGnRH-ir was found primarily in the granulated cell and central capillaries within the trabeculae. These results suggest that multiple GnRH-like peptides are present in the neural ganglia which could be the principal
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    Starvation promotes autophagy-associated maturation of the testis in the giant freshwater prawn, macrobrachium rosenbergii
    (2019-09-01) Wilairat Kankuan; Chaitip Wanichanon; Federica Morani; Sirorat Thongrod; Rossella Titone; Tanapan Siangcham; Matilde Masini; Michela Novelli; Prasert Sobhon; Ciro Isidoro; Università di Pisa; Mahidol University; Università degli Studi del Piemonte Orientale "Amedeo Avogadro"
    and cellular changes in the testis of the giant freshwater prawn Macrobrachium rosenbergii that paralleled the induction of autophagy. Under short starvation condition, the male prawns showed increased gonado-somatic index, increased size, and late stage
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    Development and roles of vitelline cells in eggshell formation in fasciola gigantica
    (2006-01-01) Ardool Meepool; Chaitip Wanichanon; Vithoon Viyanant; Prasert Sobhon; Burapha University; Mahidol University; Thammasat University
    to the uterus and the testis. Mature vitelline cells are released and transported to the Mehlis' gland-ootype complex via a series of vitelline ducts. Based on ultrastructural features, the developing vitelline cells are classified into four stages: stem cell
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    Production and characterization of a monoclonal antibody against recombinant glutathione S-transferase (GST) of Fasciola gigantica
    (2002-12-01) Witoon Khawsuk; Nantawan Soonklang; Rudi Grams; Suksiri Vichasri-Grams; Chaitip Wanichanon; Ardool Meepol; Kulathida Chaithirayanon; Pissanee Ardseungneon; Vithoon Viyanant; Suchart Edward Upathum; Praset Sobhon; Mahidol University; Thammasat University; Faculty of Medicine, Thammasat University; Burapha University
    . In addition, in adult and juvenile stages a moderate fluorescence was present in the basal layer of the tegument, while light fluorescence was observed in the caecal epithelium, cells in the ovary, testis and vitelline gland of the adult. In the metacercaria
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    Ultrastructure of the differentiating male germ cells in Haliotis asinina Linnaeus
    (2001-01-01) Prasert Sobhon; Somjai A. Wetakan; Vichai Linthong; Viriya Pankao; Chaitip Wanichanon; Ardool Meepool; Maleeya Kruatrachue; Edward Suchart Upatham; Tanate Pumthong; Mahidol University; Burapha University; Coastal Aquaculture Research and Development Center
    Male germ cells in the testis of H. asinina can be divided into 14 stages based on the ultrastructure and patterns of chromatin condensation. The spermatogonium is a spherical or oval-shaped cell with diameter about 8 μm. Its nucleus contains mostly
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    Possibility of purifying the Spermatogenic Cells in rat using Gravitational Split-Flow Thin Cell (GrSPLITT) Fractionation
    (2012) Nongnuch Tantidanai-Sungayuth; Nanthiya Poathong; Waret veerasai; Beckett, Ronald; Chaitip Wanichanon; Rapeepun Vanichviriyakit; Mahidol University. Faculty of Science. Department of Chemistry and Center of Excellence for Innovation in Chemistry
    The Gravitational split-flow thin cell (GrSPLITT) fractionation is a rapid, gentle and continuous separation technique that should be used to separate spermatogenic cells into three stages: (1) spermatogonia and spermatocyte (2) round spermatids and (3) elongate spermatids and spermatozoa. The different stages of the spermatogenic cells display differences in density and size. In this work, two modes of GrSPLITT namely, full-feed depletion mode (FFD-GrSPLITT) (and transport mode (TS-GrSPLITT) were compared for their possibility to separate the three stages of spermatogenic cells. Imaging and sizing of the original sample and all fractions were analyzed to obtain their size distribution using dark field microscopy with analysis software. The percentage of purity and recovery were calculated and used to objectively compare their separation efficiencies of the two mods of the FFD mode and the TS mode of GrSPLITT fractionations. All experiments showed that the TS-GrSPLITT fractionation trends to be the better separation efficiency than the FFDGrSPLITT fractionation.