Publication: Possibility of purifying the Spermatogenic Cells in rat using Gravitational Split-Flow Thin Cell (GrSPLITT) Fractionation
3
Issued Date
2012
Resource Type
Language
eng
ISSN
1906-2257
Rights
Mahidol University
Rights Holder(s)
Faculty of Veterinary Science Mahidol University
Bibliographic Citation
Journal of Applied Animal Science. Vol.5, No.1 (2012), 25-37
Suggested Citation
Nongnuch Tantidanai-Sungayuth, Nanthiya Poathong, Waret veerasai, Beckett, Ronald, Chaitip Wanichanon, Rapeepun Vanichviriyakit Possibility of purifying the Spermatogenic Cells in rat using Gravitational Split-Flow Thin Cell (GrSPLITT) Fractionation. Journal of Applied Animal Science. Vol.5, No.1 (2012), 25-37. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13313
Research Projects
Organizational Units
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Journal Issue
JAAS Vol. 5 No. 1
(2555)
Thesis
Title
Possibility of purifying the Spermatogenic Cells in rat using Gravitational Split-Flow Thin Cell (GrSPLITT) Fractionation
Abstract
The Gravitational split-flow thin cell (GrSPLITT) fractionation is a rapid, gentle and continuous
separation technique that should be used to separate spermatogenic cells into three stages: (1) spermatogonia
and spermatocyte (2) round spermatids and (3) elongate spermatids and spermatozoa. The different stages of
the spermatogenic cells display differences in density and size. In this work, two modes of GrSPLITT namely,
full-feed depletion mode (FFD-GrSPLITT) (and transport mode (TS-GrSPLITT) were compared for their
possibility to separate the three stages of spermatogenic cells. Imaging and sizing of the original sample and all
fractions were analyzed to obtain their size distribution using dark field microscopy with analysis software.
The percentage of purity and recovery were calculated and used to objectively compare their separation
efficiencies of the two mods of the FFD mode and the TS mode of GrSPLITT fractionations. All experiments
showed that the TS-GrSPLITT fractionation trends to be the better separation efficiency than the FFDGrSPLITT
fractionation.