Journal Issue:
JAAS Vol. 5 No. 1

Journal Volume

Journal Volume
JAAS Volume 5
(2555)

Articles

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PublicationOpen Access
ความสำคัญของสัตว์เลี้ยงเป็นเพื่อนตามแนวทาง One Health
(2555) วลาสินี มูลอามาตย์; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์. ภาควิชาเวชศาสตร์คลินิกและการสาธารณสุข
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PublicationOpen Access
Antimicrobial Susceptibility Patterns of Escherichia coli in Diarrheal Piglet Fecal Samples: Using Continuous Medicated Feed and Geographical Variation
(2012) Dusit Laohasinnarong; Wararat Thanachotsirivibul; Waree Limrungsukho; Dulyatad Gronsang; Mahidol University. Faculty of Veterinary Science
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Possibility of purifying the Spermatogenic Cells in rat using Gravitational Split-Flow Thin Cell (GrSPLITT) Fractionation
(2012) Nongnuch Tantidanai-Sungayuth; Nanthiya Poathong; Waret veerasai; Beckett, Ronald; Chaitip Wanichanon; Rapeepun Vanichviriyakit; Mahidol University. Faculty of Science. Department of Chemistry and Center of Excellence for Innovation in Chemistry
The Gravitational split-flow thin cell (GrSPLITT) fractionation is a rapid, gentle and continuous separation technique that should be used to separate spermatogenic cells into three stages: (1) spermatogonia and spermatocyte (2) round spermatids and (3) elongate spermatids and spermatozoa. The different stages of the spermatogenic cells display differences in density and size. In this work, two modes of GrSPLITT namely, full-feed depletion mode (FFD-GrSPLITT) (and transport mode (TS-GrSPLITT) were compared for their possibility to separate the three stages of spermatogenic cells. Imaging and sizing of the original sample and all fractions were analyzed to obtain their size distribution using dark field microscopy with analysis software. The percentage of purity and recovery were calculated and used to objectively compare their separation efficiencies of the two mods of the FFD mode and the TS mode of GrSPLITT fractionations. All experiments showed that the TS-GrSPLITT fractionation trends to be the better separation efficiency than the FFDGrSPLITT fractionation.
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PublicationOpen Access
Treatment of laryngeal paralysis in captive wild felids; Arytenoid lateralization technique
(2012) Phingphol Charoonrut; Sukanya Manee-in; Ruangrat Buddhirongawat; Siriporn Tangsudjai; Nareerat Sungchachai; Tipapan Parnluck; Luxsana Pasittichai; Mahidol University. Faculty of Veterinary Science
The purpose of this study was an evaluation of the result of laryngeal paralysis treatment in tigers (Panthera tigris) and lions (Panthera leo) by using arytenoid lateralization technique. Four tigers with left unilateral laryngeal paralysis, 2 tigers and 2 lion with bilateral laryngeal paralysis were included. The surgical procedure was performed by left unilateral arytenoid lateralization in all animals. The better clinical signs after operation were shown in 7 animals except 1 lion that was not improved and died 14 days after operation because of pre-operative systemic infection. One tiger died with the recurrent problem at 10 days after operation because of the laceration at the muscular process of arytenoid cartilage at prosthesis anchored. This technique used in this study can be used to treat the laryngeal paralysis in tigers and lions.
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PublicationOpen Access
Influence of dietary protein and energy levels on sow backfat thickness
(2012) Surasak Jittakhot; Dusit Laohasinnarong; Kampon Kaeoket; Mahidol University. Faculty of Veterinary Science. Department of Pre-clinical and Applied Animal Science; Mahidol University. Faculty of Veterinary Science. Department of Clinical Sciences and Public Health
The objective of the present study was to determine the influences of dietary protein and energy levels on sow backfat thickness. The study was conducted by randomly measured backfat thickness in both pregnant and lactating stages of 10,401 sows from 7 commercial herds in Thailand. The pregnant sow diets contained 14.4% crude protein (CP) and 3,346 kcal metabolizable energy (ME)/kg. The lactating sow diet contained 18.8% CP and 3,660 kcal ME/kg. The percentage of dietary protein was positively correlated with backfat thickness (r = 0.036, P < 0.01) and the metabolizable energy of the diet was positively correlated (r = 0.053, P < 0.01). Upon, stepwise method of multiple linear regression analysis showed that the percentage of dietary crude fat (X, %) was a key factor affecting the thickness of sow backfat (Y, mm) with statistical significance. The regression equation was; Y = 14.4 + (0.2X), (R2 = 0.009, P < 0.01). Conclusion, a major nutritional factor that response for changing of sow backfat thickness was the dietary energy in sow diet.

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