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Publication Metadata only Label-free guided mode resonance sensor for detection of glycated hemoglobin(2017-11-22) Boonrasri Seeleang; Chamras Promptmas; Sakoolkan Boonruang; Waleed S. Mohammed; Romuald Jolivot; Mahidol University; Thailand National Electronics and Computer Technology Center; Bangkok Universitylevel in diabetes patients. Here, the sensing approach utilizes boronate affinity by modification of the GMR surface with 3- Aminophenylboronic acid (PBA). The sensing signal is then measured via a resonance shift in a reflection spectrum as the changePublication Metadata only Boronic acid Functionalized Guided Mode Resonance Sensor for HbA1c Detection(2019-01-10) Boonrasri Seeleang; Songpol Ongwattanakul; Chamras Promptmas; Sakoolkan Boonruang; Waleed S. Mohammed; Romuald Jolivot; Mahidol University; Bangkok University; Thailand National Electronics and Computer Technology Center© 2018 IEEE. An alternative optical label-free sensor, Guided Mode Resonance (GMR), is demonstrated in this paper for detection of glycated hemoglobin (HbA1c). The GMR sensor surface is modified by cross-linking with 3-amino phenylboronic acids (3... method with a precision of 7.71-20.77% CV. This guarantees boronate-modified GMR affinity sensor for the monitoring of glycemic status in diabetes management.Publication Open Access Detection of non-amplified mycobacterium tuberculosis genomic DNA using piezoelectric DNA-based biosensors(2010) Thongchai Kaewphinit; Somchai Santiwatanakul; Chamras Promptmas; Kosum ChansiriPiezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a selfassembled monolayer method. The advantage of this study is that a non-amplified genomic bacterial DNA target was used. Instead, the genomic DNA was digested by restriction enzyme to obtain DNA fragments containing the target sequence. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex and other microorganisms. This target DNA preparation, without PCR amplification, will reduce time, costs, and the tedious step of amplification.Publication Open Access Development of piezoelectric DNA-based biosensor for direct detection of mycobacterium tuberculosis in clinical specimens(2010) Thongchai Kaewphinit; Somchai Santiwatanakul; Chamras Promptmas; Kosum ChansiriThis study was focused on establishment of piezoelectric biosensor for direct detection of Mycobacterium tuberculosis (MTB) in clinical specimens. The quartz crystal immobilized via 3-mercaptopropionic acid (MPA)/avidin/DNA biotinylated probe on gold surface and hybridization of the DNA target to DNA biotinylated probe. The optimal concentration of MPA, avidin and 5’-biotinylated DNA probe for immobilization of specific DNA probe on gold surface were 15 mM, 0.1 mg/ml and 1.5 μM, respectively. The detection of genomic DNA digestion in the range from 0.5 to 30 μg/ml. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex (MAC) and other microorganism. This target DNA preparation without amplification will reduce time consuming, costs, and the tedious step of amplification. This study can be extended to develop the new method which is high sensitivity, specificity, cheap, easy to use, and rapid for detection of MTB in many fields.