Publication: Boronic acid Functionalized Guided Mode Resonance Sensor for HbA1c Detection
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Issued Date
2019-01-10
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2-s2.0-85062109854
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Mahidol University
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SCOPUS
Bibliographic Citation
BMEiCON 2018 - 11th Biomedical Engineering International Conference. (2019)
Suggested Citation
Boonrasri Seeleang, Songpol Ongwattanakul, Chamras Promptmas, Sakoolkan Boonruang, Waleed S. Mohammed, Romuald Jolivot Boronic acid Functionalized Guided Mode Resonance Sensor for HbA1c Detection. BMEiCON 2018 - 11th Biomedical Engineering International Conference. (2019). doi:10.1109/BMEiCON.2018.8609979 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/50668
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Title
Boronic acid Functionalized Guided Mode Resonance Sensor for HbA1c Detection
Abstract
© 2018 IEEE. An alternative optical label-free sensor, Guided Mode Resonance (GMR), is demonstrated in this paper for detection of glycated hemoglobin (HbA1c). The GMR sensor surface is modified by cross-linking with 3-amino phenylboronic acids (3-APBA) using 3-(glycidyloxypropyl) trimethoxysilane (GOPTMS). 3-APBA provides a specific interaction with HbA1c under alkaline condition. The sensing scheme is performed by tracking a peak resonance in a reflection spectrum. That relies on changes in refractive index on the sensor's surface. Percent of HbA1c in total hemoglobin can be estimated using a ration of resonance shift due to absorbed HbA1c and the shift from changes in a bulk refractive index of a whole blood (Δλ HbA1c /Δλ TotalHb ). In the measurement, Lypochek diabetes controls having 5.1% (range: 4.6-5.6% measured by HPLC) and 9.5% (range: 8.5-10.4% measured by HPLC) HbA1c are used. The results from a resonance shift ration is linearly proportional to the measurement from the HPLC standard method with a precision of 7.71-20.77% CV. This guarantees boronate-modified GMR affinity sensor for the monitoring of glycemic status in diabetes management.