71 results
Search Results
Now showing 1 - 10 of 71
Publication Metadata only Vaccine potential of recombinant pro- and mature cathepsinL1 against fasciolosis gigantica in mice(2015-10-01) Pornanan Kueakhai; Narin Changklungmoa; Pannigan Chaichanasak; Wipaphorn Jaikua; Tadashi Itagaki; Prasert Sobhon; Burapha University; Mahanakorn University of Technology; Mahidol University; Iwate University. By determining the levels of IgG1 and IgG2a in the immune sera, which are indicative of Th2 and Th1 immune response, respectively, it was found that both Th1 and Th2 responses were significantly increased in rproFgCatL1- and rmatFgCatL1-immunized groups compared..., indicating that rmatFgCatL1-vaccinated mice had reduced liver parenchyma damage. The pathological lesions of liver in vaccinated groups were significantly decreased when compared with control groups. This study indicates that rFgCatL1 has a potential as aPublication Metadata only Vaccine potential of recombinant saposin-like protein 2 against Fasciolosis gigantica in mice(2013-11-12) Pornanan Kueakhai; Narin Changklungmoa; Suda Riengrojpitak; Pannigan Chaichanasak; Krai Meemon; Kulathida Chaithirayanon; Pathanin Chantree; Veerawat Sansri; Tadashi Itagaki; Prasert Sobhon; Mahidol University; Burapha University; Mahanakorn University of Technology; Iwate Universityof IgG1 and IgG2a in the immune sera, which are indicative of Th2 and Th1 immune responses, it was found that both Th1 and Th2 humoral immune response were significantly increased in rFgSAP-2 immunized group compared with the control groups, with higher... levels of Th2 (IgG1) than Th1 (IgG2a). The levels of serum aspartate aminotransferase (AST) and alanine transaminase (ALT) in rFgSAP-2-immunized group showed no significant difference from those of the non-immunized and infected group, indicatingPublication Metadata only Oogenesis and formation of cortical rods in the black tiger shrimp, Penaeus monodon(2010-03-23) Hathairat Kruevaisayawan; Rapeepun Vanichviriyakit; Wattana Weerachatyanukul; Boonsirm Withyachumnarnkul; Jittipan Chavadej; Prasert Sobhon; Mahidol University; Naresuan Universityendoplasmic reticulum (RER) in Oc1and Oc2and the presence of yolk granules and lipid droplets in Oc3all indicating active synthesis of protein and lipid components. The main characteristic of the mature oocyte (Oc4) was the presence of cortical rods (CRs...) which were composed of the tightly packed structural units each resembling a bottle-brush. Immunostaining of cortical rod proteins which were part of the structural units indicated that they were first synthesized in the RER-Golgi complex of Oc2andPublication Metadata only Molecular cloning and characterization of leucine aminopeptidase from Fasciola gigantica(2012-07-01) Narin Changklungmoa; Kulathida Chaithirayanon; Pornanan Kueakhai; Krai Meemon; Suda Riengrojpitak; Prasert Sobhon; Mahidol Universityrelated and grouped with F. hepatica LAP (FhLAP). Northern analysis showed that FgLAP transcriptional products have 1800 base pairs. Analysis by RNA in situ hybridization indicated that LAP gene was expressed in the cecal epithelial cells of adult..., and was significantly inhibited by bestatin. Its maximum activity was at pH 8.0 and enhanced by Mn 2+ ions. Localization of LAP proteins by immunohistochemistry and immunofluorescence techniques indicated that the enzyme was distributed in the apical cytoplasm of cecalPublication Metadata only The anthelmintic effect of plumbagin on Schistosoma mansoni(2013-01-01) Natcha Lorsuwannarat; Naruwan Saowakon; Pongrama Ramasoota; Chaitip Wanichanon; Prasert Sobhon; Mahidol University; Suranaree University of TechnologyThe anthelmintic effects of plumbagin (PB, 5-hydroxy-2-methyl-1,4-naphthoquinone) and praziquantel (PZQ) against adult Schistosoma mansoni in vitro were compared by estimating the relative motility (RM) values, survival indices (SI) and alterations..., leading to erosion and lesion, exposure of the basal lamina, and sloughing of the tegument. PZQ induced similar tegumental changes as those observed in PB treatment but at longer incubation time and higher doses. These data indicated that PB had morePublication Metadata only An antioxidant activity of the whole body of Holothuria scabra(2017-12-01) Saksit Nobsathian; Patoomratana Tuchinda; Prasert Sobhon; Yotsawan Tinikul; Jaruwan Poljaroen; Ruchanok Tinikul; Morakot Sroyraya; Tanes Poomton; Supakant Chaichotranunt; Mahidol University; The Coastal Fisheries Research and Development Center© The Author(s) 2016. Background: Holothuria scabra is the potential source of terpene with high antioxidant capacity and one of the most valuable species in the trade. Results: The results indicated that antioxidant activity of crude methanol... extracts and three pure compounds, Friedelin, 3-Hydroxybenzaldehyde and 4-Hydroxybenzaldehyde, from sea cucumber was determined using DPPH, Folin-Cioccalteau reagent. The results indicated the total phenolic contents at 30.52.28 ± 0.21 GAE/g dry weightPublication Metadata only Influence of aging and long-term swimming exercise on parvalbumin distribution in rat hearts(2010-01-01) Uraporn Vongvatcharanon; Kanjana Khornchatri; Wandee Udomuksorn; Ekkasit Kumarnsit; Surapong Vongvatcharanon; Prasert Sobhon; Prince of Songkla University; Faculty of Dentistry; Mahidol Universityin the 9 (P<0.05) and 18-month samples (P<0.01). Our data indicate that PV is down-regulated in the rat heart during aging. In addition, our data indicate that long-term swimming exercise could induce an increase of PV expression. © 2008 Elsevier GmbH. AllPublication Metadata only Characterization and vaccine potential of Fasciola gigantica saposin-like protein 1 (SAP-1)(2017-01-15) Pornanan Kueakhai; Narin Changklungmoa; Pinkamon Waseewiwat; Thanaporn Thanasinpaiboon; Werachon Cheukamud; Pannigan Chaichanasak; Prasert Sobhon; Burapha University; Mahanakorn University of Technology; Mahidol Universitywith non vaccinated-infected and adjuvant-infected controls, respectively. The levels of IgG1 and IgG2a specific to rFgSAP-1 in the immune sera, which are indicative of Th2 and Th1 immune responses, were inversely and significantly correlated... with the numbers of worm recoveries. The rFgSAP-1-vaccinated mice showed significantly reduced levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), and liver damage. These indicated that rFgSAP-1 has strong potential as a vaccine candidatePublication Metadata only The combined recombinant cathepsin L1H and cathepsin B3 vaccine against Fasciola gigantica infection(2021-08-01) Pornanan Kueakhai; Narin Changklungmoa; Werachon Cheukamud; Supawadee Osotprasit; Pathanin Chantree; Narin Preyavichyapugdee; Prasert Sobhon; Krai Meemon; Silpakorn University; Faculty of Medicine, Thammasat University; Mahidol University; Burapha University.0% when compared with adjuvant-infected control. These protective effects were similar among groups receiving vaccines with Alum or Freund's adjuvants. By determining the levels of IgG1 and IgG2a in the immune sera, which are indicative of Th1 and Th2... in liver pathological lesions when compared with control groups. This study indicates that the combined rproFgCatB3 and rproFgCatL1H vaccine had a high protective potential than a single a vaccine, with Alum and Freund's adjuvants showing similar levelPublication Metadata only Identification and expression of prostaglandin E synthase (PGES) gene in the central nervous system and ovary during ovarian maturation of the female mud crab, Scylla olivacea(2018-11-01) Supawadee Duangprom; Wilailuk Ampansri; Saowaros Suwansa-ard; Charoonroj Chotwiwatthanakun; Prasert Sobhon; Napamanee Kornthong; University of the Sunshine Coast; Mahidol University; Thammasat University; Burapha Universitytranscript of S. olivacea (Scyol-PGES) consists of 1258 nucleotides, which encodes for 420 amino acid PGES protein precursor. Investigation of gene expression by RT-PCR indicated that Scyol-PGES was detected in all organs studied. Based on in situ... in the neurons within the subesophageal, thoracic and abdominal ganglia. The Scyol-PGES gene expression as indicated by relative abundance of mRNA in the Stage 4 of ovarian development was greater than that at Stages 1 to 3 of ovarian development