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Publication Open Access Proteomic analysis of Chikungunya virus infected microgial cells(2012-04) Bizunesh Abere; Nitwara Wikan; Sukathida Ubol; Prasert Auewarakul; Atchara Paemanee; Suthathip Kittisenachai; Sittiruk Roytrakul; Duncan R. Smith; Mahidol University. Institute of Molecular Biosciences. Molecular Pathology Laboratory; Mahidol University. Faculty of Science. Department of Microbiology; Mahidol University. Center for Emerging and Neglected Infectious Disease.in response to infection did not support a global inhibition of either normal or IRES-mediated translation, but was consistent with the targeting of specific cellular pathways including those regulating innate antiviral mechanisms.Publication Metadata only Expression profile of human neuroblastoma cells after exposure to Naegleria fowleri(2013-04-01) Supathra Tiewcharoen; Kumchol Chaiyo; Jundee Rabablert; Sittiruk Roytrakul; Pahol Kosiyachinda; Hilmar Drechsel; Mahidol University; Silpakorn University; Thailand National Center for Genetic Engineering and Biotechnologywere identified as involved in signal transduction, translation, immune response, cytoskeleton, and stress response. We also observed changes in the abundance of these proteins after cocultivating SK-N-MC with N. fowleri, especially those involved... in angiogenesis, fatty acid metabolism, signal transduction, translation, stress response, signal pathway, and respiration. Conclusions: The proteomics analysis from this report suggested how and by which means the SK-N-MC responded after N. fowleri infection.Publication Metadata only Proteomic analysis of salinity-stressed Chlamydomonas reinhardtii revealed differential suppression and induction of a large number of important housekeeping proteins(2012-03-01) Chotika Yokthongwattana; Bancha Mahong; Sittiruk Roytrakul; Narumon Phaonaklop; Jarunya Narangajavana; Kittisak Yokthongwattana; Kasetsart University; Mahidol University; Thailand National Center for Genetic Engineering and Biotechnology; Kyung Hee Universitychaperones and proteins of the translation machinery, suggesting that they may originate from post-translational modifications rather than from de novo biosynthesis. The possible role and the salt-specific modification of these proteins by salinity stressPublication Open Access Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia(2011) Saranyoo Ponnikorn; Tasanee Panichakul; Kitima Sresanga; Chokdee Wongborisuth; Sittiruk Roytrakul; Suradej Hongeng; Sumalee Tungpradabkul; Mahidol University. Faculty of Science. Department of BiochemistryBackground: Hemoglobin E/b-thalassemia is particularly common in Southeast Asia and has variable symptoms ranging from mild to severe anemia. Previous investigations demonstrated the remarkable symptoms of bthalassemia in terms of the acceleration of apoptotic cell death. Ineffective erythropoiesis has been studied in human hematopoietic stem cells, however the distinct apoptotic mechanism was unclear. Methods: The phosphoproteome of bone marrow HSCs/CD34+ cells from HbE/b-thalassemic patients was analyzed using IMAC phosphoprotein isolation followed by LC-MS/MS detection. Decyder MS software was used to quantitate differentially expressed proteins in 3 patients and 2 normal donors. The differentially expressed proteins from HSCs/CD34+ cells were compared with HbE/b-thalassemia and normal HSCs. Results: A significant change in abundance of 229 phosphoproteins was demonstrated. Importantly, the analysis of the candidate proteins revealed a high abundance of proteins that are commonly found in apoptotic cells including cytochrome C, caspase 6 and apoptosis inducing factors. Moreover, in the HSCs patients a significant increase was observed in a specific type of phosphoserine/threonine binding protein, which is known to act as an important signal mediator for the regulation of cell survival and apoptosis in HbE/b-thalassemia. Conclusions: Our study used a novel method to investigate proteins that influence a particular pathway in a given disease or physiological condition. Ultimately, phosphoproteome profiling in HbE/b-thalassemic stem cells is an effective method to further investigate the cell death mechanism of ineffective erythropoiesis in b-thalassemia. Our report provides a comprehensive phosphoproteome, an important resource for the study of ineffective erythropoiesis and developing therapies for HbE/b-thalassemia.Publication Metadata only A proteome reference map of the causative agent of melioidosis Burkholderia pseudomallei(2011-11-24) Patompon Wongtrakoongate; Sittiruk Roytrakul; Sukkid Yasothornsrikul; Sumalee Tungpradabkul; Mahidol University; Thailand National Center for Genetic Engineering and Biotechnology; Naresuan Universityrepresenting 220 ORFs; many of them have been implicated in bacterial pathogenesis. Up to 20% of identified ORFs belong to post-translational modification and stress responses. The proteome reference map will support future analysis of the bacterial genePublication Open Access Comprehensive proteomic analysis of white blood cells from chikungunya fever patients of different severities(2014) Nitwara Wikan; Sarawut Khongwichit; Weerawat Phuklia; Sukathida Ubol; Tipparat Thonsakulprasert; Montri Thannagith; Duangrudee Tanramluk; Atchara Paemanee; Suthathip Kittisenachai; Sittiruk Roytrakul; Smith, Duncan R; Mahidol University. Institute of Molecular Biosciences; Mahidol University. Center for Emerging and Neglected Infectious DiseasesBackground: Chikungunya fever (CHIKF) is a recently re-emerged mosquito transmitted viral disease caused by the chikungunya virus (CHIKV), an Alphavirus belonging to the family Togaviridae. Infection of humans with CHIKV can result in CHIKF of variable severity, although the factors mediating disease severity remain poorly defined. Methods: White blood cells were isolated from blood samples collected during the 2009-2010 CHIKF outbreak in Thailand. Clinical presentation and viral load data were used to classify samples into three groups, namely non chikungunya fever (non-CHIKF), mild CHIKF, and severe CHIKF. Five samples from each group were analyzed for protein expression by GeLC-MS/MS. Results: CHIKV proteins (structural and non-structural) were found only in CHIKF samples. A total of 3505 human proteins were identified, with 68 proteins only present in non-CHIKF samples. A total of 240 proteins were found only in CHIKF samples, of which 65 and 46 were found only in mild and severe CHIKF samples respectively. Proteins with altered expression mapped predominantly to cellular signaling pathways (including toll-like receptor and PI3K-Akt signaling) although many other processes showed altered expression as a result of CHIKV infection. Expression of proteins consistent with the activation of the inflammasome was detected, and quantitation of (pro)-caspase 1 at the protein and RNA levels showed an association with disease severity. Conclusions: This study confirms the infection of at least a component of white blood cells by CHIKV, and shows that CHIKV infection results in activation of the inflammasome in a manner that is associated with disease severity.Publication Metadata only Alteration in pancreatic protein expression in dexamethasone-treated mice(2020-01-01) Amod Sharma; Sittiruk Roytrakul; Suthathip Kittisenachai; Namoiy Semprasert; Suwattanee Kooptiwut; Thailand National Center for Genetic Engineering and Biotechnology; Faculty of Medicine, Siriraj Hospital, Mahidol Universityproteins mainly belonged to cell redox homeostasis, metabolism, endoplasmic reticulum stress, translation, and signaling pathways.Publication Metadata only Shotgun proteomics analysis of proliferating STRO-1-positive human dental pulp cell after exposure to nacreous water-soluble matrix(2015-01-01) Titikan Laothumthut; Jeeraphat Jantarat; Atchara Paemanee; Sittiruk Roytrakul; Panjit Chunhabundit; Mahidol University; Thailand National Center for Genetic Engineering and Biotechnology, signal transduction, stress response, transcription, translation, and transport. Conclusion: These results indicate that WSM of P. maxima has the ability to induce proliferation of human dental pulp cells. Clinical relevance: This finding initiatedPublication Metadata only A Rapid and Simple Method for Construction and Expression of a Synthetic Human Growth Hormone Gene in Escherichia coli(2001-11-30) Sittiruk Roytrakul; Lily Eurwilaichitr; Chittiwat Suprasongsin; Sakol Panyim; Thailand National Center for Genetic Engineering and Biotechnology; Mahidol University; Faculty of Medicine, Ramathibodi Hospital, Mahidol Universitythe gene to facilitate mutagenesis in further studies. To minimize an initiation translation problem, a 624-bp cassette that contained a ribosome binding site and a start codon were fused to the hGH-coding sequence that was flanked between the EcoPublication Metadata only Proteomic analysis of chikungunya virus infected microgial cells(2012-04-13) Bizunesh Abere; Nitwara Wikan; Sukathida Ubol; Prasert Auewarakul; Atchara Paemanee; Suthathip Kittisenachai; Sittiruk Roytrakul; Duncan R. Smith; Mahidol University; Thailand National Center for Genetic Engineering and Biotechnologyin response to infection did not support a global inhibition of either normal or IRES-mediated translation, but was consistent with the targeting of specific cellular pathways including those regulating innate antiviral mechanisms. © 2012 Abere et al.
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