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Publication Metadata only Development of a microarray for identification of pathogenic Clostridium spp.(2010-02-01) Tavan Janvilisri; Joy Scaria; Robin Gleed; Susan Fubini; Michelle M. Bonkosky; Yrjö T. Gröhn; Yung Fu Chang; Cornell University; Oak Ridge Institute for Science and Education; Mahidol Universitytechniques for clostridia are laborious, are time consuming, and may adversely affect the therapeutic outcome. In this study, we developed an oligonucleotide diagnostic microarray for pathogenic Clostridium spp. The microarray specificity was tested against... 65 Clostridium isolates. The applicability of this microarray in a clinical setting was assessed with the use of mock stool samples. The microarray was successful in discriminating at least 4 species with the limit of detection as low as 104CFU/mPublication Metadata only Microarray identification of Clostridium difficile core components and divergent regions associated with host origin(2009-06-01) Tavan Janvilisri; Joy Scaria; Angela D. Thompson; Ainsley Nicholson; Brandi M. Limbago; Luis G. Arroyo; J. Glenn Songer; Yrjö T. Gröhn; Yung Fu Chang; Cornell University; National Center for Emerging and Zoonotic Infectious Diseases; Ontario Veterinary College; University of Arizona; Mahidol Universityevaluated the genomic content of 73 C. difficile strains isolated from humans, horses, cattle, and pigs by comparative genomic hybridization with microarrays containing coding sequences from C. difficile strains 630 and QCD-32g58. The sequenced genome of CPublication Metadata only Clostridium difficile transcriptome analysis using pig ligated loop model reveals modulation of pathways not modulated in vitro(2011-06-01) Joy Scaria; Tavan Janvilisri; Susan Fubini; Robin D. Gleed; Sean P. McDonough; Yung Fu Chang; Cornell University; Mahidol UniversityA pig ligated loop model was used to analyze the in vivo transcriptome response of Clostridium difficile. Bacterial RNA from the loops was retrieved at different times and was used for microarray analysis. Several virulence-associated genes... and genes involved in sporulation cascade were differentially expressed (DE). In concordance with observed upregulation of toxin genes in microarray, enzyme-linked immunosorbent assay estimation of total toxin showed high amounts of toxin in the loopsPublication Metadata only Dysregulation of microRNA in cholangiocarcinoma identified through a meta-analysis of microRNA profiling(2020-08-07) Somsak Likhitrattanapisal; Supeecha Kumkate; Pravech Ajawatanawong; Kanokpan Wongprasert; Rutaiwan Tohtong; Tavan Janvilisri; Mahidol University; Thailand National Center for Genetic Engineering and Biotechnology; Faculty of Medicine, Siriraj Hospital, Mahidol University-analysis was conducted upon the pooled miRNA microarray data of cholangiocarcinoma (CCA). AIM To identify differentially expressed (DE) miRNAs and perform functional analyses in order to gain insights to understanding miRNA-target interactions involved... in tumorigenesis pathways of CCA. METHODS Raw data from 8 CCA miRNA microarray datasets, consisting of 443 samples in total, were integrated and statistically analyzed to identify DE miRNAs via comparison of levels of miRNA expression between CCA and normal bilePublication Metadata only Wnt8b as an independent prognostic marker for nasopharyngeal carcinoma(2021-08-01) Chawalit Ngernsombat; Pongphol Prattapong; Noppadol Larbcharoensub; Krittika Khotthong; Tavan Janvilisri; Ramathibodi Hospital; Mahidol University; N Health Pathology LaboratoryBackground: Members of the Wnt signaling pathway have been shown to play a role in nasopharyngeal carcinoma (NPC) progression. Aim: The purpose of this study was to investigate WNT8B protein expression in NPC patients using tissue microarray (TMAPublication Metadata only Transcriptome meta-analysis reveals dysregulated pathways in nasopharyngeal carcinoma(2015-08-24) Warut Tulalamba; Noppadol Larbcharoensub; Ekaphop Sirachainan; Aunchalee Tantiwetrueangdet; Tavan Janvilisri; Mahidol Universitymeta-analysis of NPC transcriptome data was performed to determine dysregulated pathways, potentially leading to identification of molecular markers. Ten independent NPC gene expression profiling microarray datasets, including 135 samples from NPC cellPublication Metadata only Meta-analysis of gene expression profiles identifies differential biomarkers for hepatocellular carcinoma and cholangiocarcinoma(2016-09-01) Somsak Likhitrattanapisal; Jaitip Tipanee; Tavan Janvilisri; Mahidol Universityof phenotypic characteristics, thus leading to difficulties in HCC and CCA differential diagnoses. In this study, a transcriptome meta-analysis was performed on HCC and CCA microarray data to identify differential transcriptome networks and potential biomarkersPublication Metadata only Analysis of ultra low genome conservation in Clostridium difficile(2010-12-20) Joy Scaria; Lalit Ponnala; Tavan Janvilisri; Weiwei Yan; Lukas A. Mueller; Yung Fu Chang; Cornell University; Mahidol University; Boyce Thompson Institute for Plant ResearchMicroarray-based comparative genome hybridisations (CGH) and genome sequencing of Clostridium difficile isolates have shown that the genomes of this species are highly variable. To further characterize their genome variation, we employed integrationPublication Open Access CD2068 potentially mediates multidrug efflux in Clostridium difficile(2017) Chawalit Ngernsombat; Suthasinee Sreesai; Phurt Harnvoravongchai; Surang Chankhamhaengdecha; Tavan Janvilisri; Mahidol University. Faculty of Science. Department of Biochemistry; Mahidol University. Faculty of Science. Department of BiologyClostridium difficile is a major cause of antibiotic-associated diarrhea and the treatment thereof becomes more difficult owing to a rise of multidrug resistant strains. ATP-binding cassette (ABC) transporters are known to play a crucial role in the resistance to multiple antibiotics. In this study, the potential contribution of an ABC transporter in C. difficile multidrug resistance was investigated. The expression level of the cd2068 gene in C. difficile encoding an ABC transporter was up-regulated following the exposure to certain antibiotics compared to the control cells. Heterologous expression of CD2068 in Escherichia coli revealed that it mediated the efflux of fluorescent substrates and conferred resistance to multiple drugs. The CD2068-associated ATPase activity in membrane vesicles was also stimulated by various antibiotics. Furthermore, the insertional inactivation of the cd2068 gene in C. difficile led to a significant increase in susceptibility to antibiotics, which could be genetically complemented, supporting that CD2068 was directly associated to the drug resistance. These results demonstrate the potential role for the ABC transporter CD2068 in the resistance mechanism against multiple drugs in C. difficile.