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Publication Metadata only In vitro drug sensitivity testing in Plasmodium vivax(2008-10-01) Walther H. Wernsdorfer; Oumaporn Tasanor; Gunther Wernsdorfer; Medizinische Universitat Wien; Thammasat University; Mahidol UniversityThe possibility of short-term in vitro cultivation, i.e. growth of asexual erythrocytic stages up to the stage of mature schizonts, permits in principle the development of drug sensitivity tests also for Plasmodium vivax. In the absence of a... sequestration of erythrocytes carrying the advanced stages of schizogony, asexual parasites of all stages may be seen in the peripheral blood of patients infected with P. vivax. This precludes schizont maturation tests since schizont development will be undulyPublication Metadata only In-vitro sensitivity testing of Plasmodium vivax: Response to lumefantrine and chloroquine in northwestern Thailand(2003-12-01) Daniela Lux; Somsak Prajakwong; Herwig Kollaritsch; Gunther Wernsdorfer; Walther H. Wernsdorfer; Universitat Wien; Off. of Vector-Borne Disease Control; Mahidol University; Dept. Specific Prophylaxis/Trop. M.response of P. vivax. In this study, a recently developed in-vitro microtechnique for the assessment of the parasite's sensitivity to chloroquine was adapted to and validated for lumefantrine. In 21 P. vivax isolates tested at Mae Hong Son, northwesternPublication Metadata only An in vitro system for assessing the sensitivity of Plasmodium vivax to chloroquine(2002-06-19) Oumaporn Tasanor; Harald Noedl; Kesara Na-Bangchang; Kanungnit Congpuong; Jeeraphat Sirichaisinthop; Walther H. Wernsdorfer; Mahidol University; Universitat Wien; Thammasat University; Thailand Ministry of Public Health; Office of Vector-Borne Disease ControlFollowing earlier observations on short-term culture of Plasmodium vivax, an in vitro test system has been developed for assessing the parasite's sensitivity to chloroquine. Fresh isolates with predominantly young trophozoites are diluted 1:19... was generally insufficient for an adequate development of the parasites. The reading of the test is based on stage-specific differential counts in the Giemsa-stained pre-incubation and post-incubation thick films, the evaluation on log-probit analysis of drugPublication Metadata only In vitro activity of artemisinin alone and in combination with retinol against Plasmodium falciparum(2005-01-01) Kamala Thriemer; Gunther Wernsdorfer; Chaiporn Rojanawatsirivet; Herwig Kollaritsch; Jeeraphat Sirichainsinthop; Walther H. Wernsdorfer; Medizinische Universitat Wien; Mahidol University; Thailand Ministry of Public HealthIncreasing resistance of Plasmodium falciparum to antimalarial drugs is an important public health problem, demanding novel therapeutic approaches. This study had the objective of assessing the in vitro activity of artemisinin and its combination... with retinol in fresh isolates of P. falciparum in an area with a high proportion of multi-drug resistant strains. The tests were based on the inhibition of schizont maturation. In 45 successfully tested isolates, the mean effective concentrations (ECsPublication Metadata only Malaria drug-susceptibility testing. HRP2-based assays: Current data, future perspectives(2003-12-01) Harald Noedl; Walther H. Wernsdorfer; Herwig Kollaritsch; Sornchai Looareesuwan; Robert S. Miller; Chansuda Wongsrichanalai; Universitat Wien; Armed Forces Research Institute of Medical Sciences, Thailand; Mahidol University; Abt. Spezifische Prophylaxe/T.In past decades, malaria in-vitro drug-susceptibility assays have become an indispensable tool for the development of novel drugs, as well as for the surveillance of antimalarial drug resistance. The traditional in-vitro assays, however, remain... to perform and requires little technical equipment. In our experiments with laboratory strains of P. falciparum against common antimalarials, the results closely parallel those obtained from the isotope assay and from WHO schizont maturation tests (P < 0Publication Metadata only Comparison of the in-vitro activity of amodiaquine and its main metabolite, monodesethyl-amodiaquine, in Plasmodium falciparum(2003-12-01) Ulrich Gerstner; Somsak Prajakwong; Gerhard Wiedermann; Jeeraphat Sirichaisinthop; Gunther Wernsdorfer; Walther H. Wernsdorfer; Universitat Wien; Off. of Vector-Borne Disease Control; Mahidol University; Dept. Specific Prophylaxis/Trop. M.tested, in parallel, for their in-vitro sensitivity to both compounds, using the WHO micro-test Mark II, measuring the inhibition of schizont maturation. The geometric mean cut-off concentrations of schizont maturation were 1826nM (related to bloodPublication Metadata only Synergism of desbutyl-benflumetol and retinol against Plasmodium falciparum in vitro(2005-01-01) Doris Samal; Chaiporn Rojanawatsirivet; Gunther Wernsdorfer; Herwig Kollaritsch; Jeeraphat Sirichaisinthop; Walther H. Wernsdorfer; Medizinische Universitat Wien; Thailand Ministry of Public Health; Mahidol UniversityThis in vitro study was conducted to assess the blood schizontocidal activity of Desbutyl-benflumetol (DBB), a new benzindene derivative, retinol and a combination of both compounds. The tests were carried out according to the methodology of the WHO... standard test Mark II, measuring the drug-dependant inhibition of schizont maturation, and using 43 fresh isolates of Plasmodium falciparum from northwestern Thailand, an area with established multidrug-resistance. DBB and retinol showed a mean 50Publication Metadata only Synergistic interaction between atovaquone and retinol in Plasmodium falciparum in vitro(2007-11-01) Bettina Exner; Gunther Wernsdorfer; Jeeraphat Sirichaisinthop; Chaiporn Rojanawatsirivet; Herwig Kollaritsch; Walther H. Wernsdorfer; Medizinische Universitat Wien; Mahidol University; Thailand Ministry of Public Healthof the physiological serum retinol levels. The in vitro tests followed the WHO standard protocol Mark II for measuring the inhibition of schizont maturation in Plasmodium falciparum. Valid results for all 5 test lines were obtained with 26 fresh parasite isolates fromPublication Metadata only In vitro interaction between artemisinin and chloroquine as well as desbutyl-benflumetol in Plasmodium vivax(2006-12-01) Leila Kyavar; Chaiporn Rojanawatsirivet; Herwig Kollaritsch; Gunther Wernsdorfer; Jeeraphat Sirichaisinthop; Walther H. Wernsdorfer; Medizinische Universitat Wien; Thailand Ministry of Public Health; Mahidol Universitybetween artimisinin and chloroquine with a view of a potential acceleration of the clinical-parasitological response, and the investigation of therapeutic alternatives in the event of chloroquine resistance in Plasmodium vivax. Tests were based... on the growth inhibition of Plasmodium vivax, determined by morphological differential counts of 200 asexual parasites. In total 45 isolates were evaluated successfully with parallel tests for artemisinin, chloroquine and desbutyl-benflumetol (DBB) alonePublication Metadata only Comparative study on the in vitro activity of lumefantrine and desbutyl-benflumetol in fresh isolates of Plasmodium vivax from Thailand(2004-12-01) Daniela K. Pirker-Krassnig; Gunther Wernsdorfer; Jeeraphat Sirichaisinthop; Chaiporn Rojanawatsirivet; Herwig Kollaritsch; Walther H. Wernsdorfer; Medizinische Universitat Wien; Mahidol University; Thailand Ministry of Public Health; Institute for Specific Prophylaxis and Tropical Medicinein northwestern Thailand near the border to Myanmar. Parallel in-vitro tests with lumefantrine and desbutyl-benflumetol were carried out with 53 fresh isolates of P. vivax. For both compounds, the parasite showed a homogenous, log-normal inhibition pattern...The occurrence of chloroquine resistance in Plasmodium vivax underlines the need for monitoring the drug response of this important malaria parasite and for the evaluation of alternative therapeutic agents. In-vitro methods facilitate these tasks
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