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Item Metadata only Production and characterization of the recombinant capsid protein of dengue-2 virus in E.coli(Mahidol University. Mahidol University Library and Knowledge Center, 2004) Prapapun Ong-ajchaowlerd; Prida MalasitItem Metadata only Cloning, expression and polyclonal antibodies production against recombinant VP19 envelope and VP26 capsid proteins of white spot syndrome virus (WSSV) in black tiger shrimp (Penaeus monodon)(Mahidol University. Mahidol University Library and Knowledge Center, 2005) Phiromsak Phattanapaijitkun; Nitaya Thammapalerd; Paisarn Sithigorngul; Parin Chaivisuthangkura; Somchai Awakairt; Siwaporn Longyant) to detect white spot syndrome virus. Structural gene of envelope protein VP19 was cloned into expression vector (pMAL-C2) and expressed in E. coli (BL21) as a fusion protein, MBP-VP19 with a M.W. of 55.5 kDa. For WSSV capsid protein VP26, fusion protein... with 6-histidine tag, 6 x His- VP 26, was used to generate polyclonal antibodies. The bacterial expression system allowed the production of 10 mg of purified recombinant proteins per liter of bacterial culture. Both recombinant proteins were usedItem Open Access Development of quantitative polymerase chain reaction assay for detection of scale drop disease virus (SDDV) in Asian sea bass(Mahidol University, 2021) Sukhontip Sriisan; Chuenchit Boonchird; Siripong Thitamadee; Saengchan SenapinGreen qPCR assay for rapid detection of SDDV. Specific primers targeting a 135-bp fragment of ATPase coding gene and a 121-bp fragment of major capsid protein (MCP) coding gene of the SDDV genome were newly designed. The optimized qPCR conditions usingItem Metadata only Development of BKV-quantum dot nanoparticles (QDS) based on lateral flow chromatographic test strip(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Tanapon Sioloetwong; Toemsak Srikhirin; Nunkanya Sathilapongsautti; Atcha KopwittayaBK virus (BKV) is a virus in the polyomavirus family that have a characteristic structure such as an icosahedral shape and a non-envelop capsid structure. This virus can develop into inflammatory diseases such as pneumonia, nephritis, etc... of streptavidin for increasing the signal of detection, because many works of literature applied the high concentration of streptavidin to detect by dispenser machine which can reduce the interfering reaction with high concentration of protein loading; (2) The DNA