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Publication Metadata only Cloning and expression of penicillin acylase genes from overproducing strains of Escherichia coli and Bacillus megaterium(1987-01-01) V. Meevootisom; J. R. Saunders; Mahidol University; University of LiverpoolPenicillin acylase genes from Escherichia coli 194, of an overproducing mutant (194-3) of this strain, and of a similar overproducing mutant of Bacillus megaterium UN1 were cloned in E. coli DH1 on the plasmid vector pACYC184. The sizes... in the original strains. The Bacillus penicillin acylase was produced intracellularly in E. coli recombinants, which is in contrast to the normal extracellular production of this enzyme in B. megaterium. Recombinant plasmids containing penicillin acylase genesPublication Metadata only High expression of the penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its own pac minus mutant(2000-08-15) W. Panbangred; K. Weeradechapon; S. Udomvaraphant; K. Fujiyama; V. Meevootisom; Mahidol University; Osaka UniversityBy marker exchange mutagenesis, Bacillus megaterium strain UN-1 (Bm-UN1) was used to prepare a mutant strain B. megaterium UN-cat (Bm-UNcat) lacking the penicillin G acylase gene (pac). The pac gene from Bm-UN1 was subcloned into pTF6... and the resultant plasmid, pBA402, was introduced into Bm-UNcat and Bacillus subtilis. Bm-UNcat harbouring pBA402 produced high penicillin G acylase (PAC) activity of 13.7, 19.5 and 20.4 U ml1 at 24, 36 and 48 h of culture, respectively. This was two- to fivefoldPublication Metadata only Biodegradation of Bisphenol A by a Newly Isolated Bacillus megaterium Strain ISO-2 from a Polycarbonate Industrial Wastewater(2018-11-01) B. Suyamud; D. Inthorn; B. Panyapinyopol; P. Thiravetyan; South Carolina Commission on Higher Education; Mahidol University; King Mongkut s University of Technology Thonburi© 2018, Springer Nature Switzerland AG. A novel bacterium, Bacillus megaterium strain ISO-2, capable of the degradation of bisphenol A (BPA), was isolated from wastewater collected from a polycarbonate industry. The bacterium, which was grown...-MS) and liquid chromatography-mass spectrometry (LC-MS) analysis revealed several intermediates during the BPA degradation process by B. megaterium strain ISO-2. These intermediates were identified as 4-(2-hydroxypropan-2-yl)phenol, 4-isopropylphenolPublication Metadata only Improved X-ray diffraction from Bacillus megaterium penicillin G acylase crystals through long cryosoaking dehydration(2011-12-01) Catleya Rojviriya; Thunyaluck Pratumrat; Mark A. Saper; Jirundon Yuvaniyama; Mahidol University; University Michigan Ann ArborPenicillin G acylase from Bacillus megaterium (BmPGA) is currently used in the pharmaceutical industry as an alternative to PGA from Escherichia coli (EcPGA) for the hydrolysis of penicillin G to produce 6-aminopenicillanic acid (6-APA), a penamPublication Metadata only Characterization of two siderophores produced by Bacillus megaterium: A preliminary investigation into their potential as therapeutic agents(2020-10-01) Hataichanok Chuljerm; Manu Deeudom; Suthat Fucharoen; Francesca Mazzacuva; Robert C. Hider; Somdet Srichairatanakool; Agostino Cilibrizzi; Mahidol University; King's College London; Chiang Mai University© 2020 Elsevier B.V. Background: Microorganisms produce siderophores in order to scavenge iron from the environment and this study focuses on the characterization of the two siderophores secreted by Bacillus megaterium. The general biologicalPublication Metadata only Characterization, gene cloning, and heterologous expression of β-mannanase from a thermophilic Bacillus subtilis(2011-02-01) Pijug Summpunn; Suttidarak Chaijan; Duangnate Isarangkul; Suthep Wiyakrutta; Vithaya Meevootisom; Mahidol UniversityBacillus subtilis BCC41051 producing a thermostable β-mannanase was isolated from soybean meal-enriched soil and was unexpectedly found to be thermophilic in nature. The extracellular β-mannanase (ManA) produced was hydrophilic...A encoded a protein of 362 amino acid residues, with the first 26 residues identified as a signal peptide. High expression of recombinant ManA was achieved in both Escherichia coli BL21 (DE3) (415. 18 U/ml) and B. megaterium UNcat (359 U/ml). © 2011Publication Unknown Titanium and iron dissolutions from ilmenite by acid leaching and microbiological oxidation techniques(2013-05-01) Woranart Jonglertjunya; Theeraporn Rubcumintara; Mahidol UniversityThis research was concerned with the solubility of ilmenite (Tin Tailing) in acids (sulfuric, hydrochloric, oxalic and citric) and bioleaching by Aspergillus niger, Penicillium citrinum and Bacillus megaterium. Ilmenite concentrate was soluble in 1... low (<0.28% extraction). For the bioleaching, B. megaterium was subcultured in solution in a shake flask containing nutrient broth. The flasks were incubated in a rotary shaker at 30 °C and 200 rpm. The bioleaching experiments after 3 days gavePublication Unknown Domain inhibitory and bacteriostatic activities of the five-domain Kazal-type serine proteinase inhibitor from black tiger shrimp Penaeus monodon(2009-04-01) Suchao Donpudsa; Anchalee Tassanakajon; Vichien Rimphanitchayakit; Mahidol University. Domain 2 inhibited also elastase. Domain 4 weakly inhibited subtilisin and trypsin. The intact SPIPm2 inhibitor was found to possess bacteriostatic activity against the Bacillus subtilis but not the Bacillus megaterium, Staphylococcus aureus, VibrioPublication Unknown Antibacterial and antiproteinase activities of a double whey acidic protein domain-containing protein from Penaeus vannamei Boone, 1931 (Decapoda, Penaeidae)(2020-01-01) Taksaorn Sakunwattana; Phattarunda Jaree; Vichien Rimphanitchayakit; Anchalee Tassanakajon; Sirinit Tharntada; Chulalongkorn University; Kasetsart University; Mahidol Universityand tested for its deleterious activity against the bacteria. The rPvDWD1 protein exhibited growth inhibitory activity against Bacillus megaterium and VPAHPND. Moreover, the rPvDWD1 protein possessed an antiproteinase activity against the secreted proteinases... from Bacillus subtilis and VPAHPND. The PvDWD1 perhaps functions in shrimp immunity through antimicrobial and antiproteinase properties.Publication Metadata only Kazal-type serine proteinase inhibitors from the black tiger shrimp Penaeus monodon and the inhibitory activities of SPIPm4 and 5(2009-08-01) Suwattana Visetnan; Suchao Donpudsa; Premruethai Supungul; Anchalee Tassanakajon; Vichien Rimphanitchayakit; Mahidol University; Thailand National Center for Genetic Engineering and Biotechnologyagainst Gram-positive bacteria: Bacillus subtilis, Bacillus megaterium, Staphylococcus aureus, and Gram-negative bacteria: Vibrio harveyi 639, E. coli JM109. Gene expression study revealed that the SPIPm5 gene was up-regulated in response to heat treatment