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Publication Metadata only Caspase-4 Mediates Restriction of Burkholderia pseudomallei in Human Alveolar Epithelial Cells(2020-02-20) Chanya Srisaowakarn; Matsayapan Pudla; Marisa Ponpuak; Pongsak Utaisincharoen; Mahidol Universitycaspases. Although the noncanonical inflammasome (caspase-11) has been investigated in a murine model of B. pseudomallei infection, its role in humans, particularly in lung epithelial cells, remains unknown. We, therefore, investigated the function... of caspase-4 (ortholog of murine caspase-11) in intracellular killing of B. pseudomallei The results showed that B. pseudomallei induced caspase-4 activation at 12 h postinfection in human alveolar epithelial A549 cells. The number of intracellular BPublication Metadata only Emetine regulates the alternative splicing of caspase 9 in tumor cells(2011-11-01) Danmin Pan; Kritsanapol Boon-Unge; Piyarat Govitrapong; Jianhua Zhou; Nantong University; Mahidol University; University of Massachusetts Medical SchoolExons 3 to 6 in the caspase 9 gene undergo alternative splicing in which the larger caspase 9 splice variant promotes apoptosis, in contrast to the dominant negative anti-apoptotic splice variant, the smaller caspase 9b. In this study...RNA and concomitantly decreased the mRNA level of larger caspase 9 in a dose- and time-dependent manner, indicating that Emetine desensitizes C33A, MCF-7 and MCF-7/Adr to cell death. In contrast, treatment of PC3 cells, a prostate cancer cell line, manifestedPublication Metadata only Caspase-1 mediates resistance in murine melioidosis(2009-04-01) Katrin Breitbach; Guang Wen Sun; Jens Köhler; Kristin Eske; Patimaporn Wongprompitak; Gladys Tan; Yichun Liu; Yunn Hwen Gan; Ivo Steinmetz; Ernst-Moritz-Arndt-Universitat Greifswald; National University of Singapore; Mahidol University; DSO National Laboratories-γ) and interleukin-18 cytokine levels 24 h after infection compared to control animals. caspase-1-/- bone marrow- derived macrophages (BMM) exhibited strong caspase-3 expression and reduced cell damage compared to wild-type (WT) cells during early B.... pseudomallei infection, indicating "classical" apoptosis, whereas WT BMM showed signs of rapid caspase-1-dependent cell death. Moreover, we found that caspase-1-/- BMM had a strongly increased bacterial burden compared to WT cells 3 h after infection underPublication Metadata only Caspase-1-Dependent and -Independent Cell Death Pathways in Burkholderia pseudomallei Infection of Macrophages(2014-01-01) Antje Bast; Kathrin Krause; Imke H.E. Schmidt; Matsayapan Pudla; Stefanie Brakopp; Verena Hopf; Katrin Breitbach; Ivo Steinmetz; Friedrich-Loeffler-Institute; Mahidol Universityprocessing and pyroptosis is exclusively dependent on NLRC4, but not on NLRP3 in the early phase of macrophage infection, whereas at later time points caspase-1 activation and cell death is NLRC4- independent. In the early phase we identified an activation.... The early activation pathway of caspase-1 in macrophages was markedly reduced or completely abolished after infection with a B. pseudomallei flagellin FliC or a T3SS3 BsaU mutant. Studies using cells transfected with the wild-type and mutated T3SS3 effectorPublication Metadata only Enhanced expression of Fas and FasL modulates apoptosis in the lungs of severe P. falciparum malaria patients with pulmonary edema(2015-01-01) Chuchard Punsawad; Parnpen Viriyavejakul; Chayanee Setthapramote; Sarawoot Palipoch; Walailak University; Mahidol University; Vajira Hospital, including cleaved caspase-3 and cleaved caspase-8 in the lung tissues were investigated by the immunohistochemistry (IHC) method. Semi-quantitative analysis of IHC staining revealed that cellular expression of Fas, FasL, cleaved caspase-8, and cleaved... caspase-3 were significantly increased in the lungs of patients with PE compared with the lungs of patients with non-PE and control groups (all P < 0.05). In addition, significant positive correlations were obtained between Fas and apoptosis (rs= 0.937, PPublication Metadata only Cloning and characterization of a caspase gene from black tiger shrimp (Penaeus monodon)-infected with white spot syndrome virus (WSSV)(2007-08-01) Kanokpan Wongprasert; Pakkakul Sangsuriya; Amornrat Phongdara; Saengchan Senapin; Mahidol University; Prince of Songkla University; Thailand National Center for Genetic Engineering and BiotechnologyICE protein of Drosophila melanogaster, and it exhibited caspase-3 activity in vitro. PmCasp was cloned and expressed in Escherichia coli and a rabbit polyclonal antiserum was produced. In Western blots, the antiserum reacted with purified recombinant Pm.... Immunohistochemical analysis of gills from the WSSV-infected shrimp revealed immunoreactivity localized in the cytoplasm of both normal and apparently apoptotic cells. In summary, a caspase-3 like gene is conserved in P. monodon and is up-regulated after WSSVPublication Metadata only Two new anti-apoptotic proteins of white spot syndrome virus that bind to an effector caspase (PmCasp) of the giant tiger shrimp Penaeus (Penaeus) monodon(2014-01-01) Tareerat Lertwimol; Pakkakul Sangsuriya; Kornsunee Phiwsaiya; Saengchan Senapin; Amornrat Phongdara; Chuenchit Boonchird; Timothy W. Flegel; Mahidol University; Chulalongkorn University; Thailand National Center for Genetic Engineering and Biotechnology; Prince of Songkla UniversityWhite spot syndrome virus proteins WSSV134 and WSSV322 have been shown to bind with the p20 domain (residues 55-214) of Penaeus monodon caspase ( PmCasp) protein through yeast two-hybrid screening. Binding was confirmed for the p20 domain... and the full-length caspase by co-immunoprecipitation. WSSV134 is also known as the WSSV structural protein VP36A, but no function or conserved domains have been ascribed to WSSV322. Discovery of the caspase binding activity of these two proteins ledPublication Metadata only Overexpression of survivin and caspase 3 in oral carcinogenesis(2014-01-01) Sopee Poomsawat; Jirapa Punyasingh; Paisarn Vejchapipat; Mahidol University; Chulalongkorn UniversitySurvivin is an inhibitor of apoptosis protein that inhibits caspase 3 function. While cytoplasmic survivin suppresses apoptosis, nuclear survivin regulates cell division. Little is known about the subcellular localization of survivin in oral... carcinogenesis. This study examined the subcellular distribution of these 2 proteins in oral squamous cell carcinoma (OSCC) and premalignant lesions including oral leukoplakia (OL) with and without dysplasia. Expression of survivin and caspase 3 werePublication Metadata only Caspase-3, a shrimp phosphorylated hemocytic protein is necessary to control YHV infection(2021-07-01) Phattara orn Havanapan; Suparat Taengchaiyaphum; Atchara Paemanee; Nuanwan Phungthanom; Sittiruk Roytrakul; Kallaya Sritunyalucksana; Chartchai Krittanai; Thailand National Center for Genetic Engineering and Biotechnology; Institute of Molecular Biosciences, Mahidol University; Thailand National Science and Technology Development Agencyon identified as caspase-3 in YHV-infected GC is most interesting. Blocking caspase-3 function using a specific inhibitor (Ac-DEVD-CMK) demonstrated high replication of YHV and consequently, high shrimp mortality. The immunohistochemistry results confirmed... the high viral load in shrimp that caspase-3 activity was blocked. Caspase-3 is regulated through a variety of posttranslational modifications, including phosphorylation. Analysis of phosphorylation sites of shrimp caspase-3 revealed phosphorylation sitesPublication Metadata only Proteinase 3-dependent caspase-3 cleavage modulates neutrophil death and inflammation(2014-01-01) Fabien Loison; Haiyan Zhu; Kutay Karatepe; Anongnard Kasorn; Peng Liu; Keqiang Ye; Jiaxi Zhou; Shannan Cao; Haiyan Gong; Dieter E. Jenne; Eileen Remold-O'Donnell; Yuanfu Xu; Hongbo R. Luo; Children's Hospital Boston; Mahidol University; Institute of Hematology and Blood Disease Hospital; Srinakharinwirot University; Emory University; Helmholtz Center Munich German Research Center for Environmental HealthCaspase-3-mediated spontaneous death in neutrophils is a prototype of programmed cell death and is critical for modulating physiopathological inflammatory responses; however, the underlying regulatory pathways remain ill defined. Here we determined... that in aging neutrophils, the cleavage and activation of caspase-3 is independent of the canonical caspase-8- or caspase-9-mediated pathway. Instead, caspase-3 activation was mediated by serine protease proteinase 3(PR3), which is present in the cytosol