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Publication Metadata only Development of multiplex PCR for the detection of total coliform bacteria for Escherichia coli and Clostridium perfringens in drinking water(2005-01-01) Suwalee Tantawiwat; Unchalee Tansuphasiri; Waranya Wongwit; Varee Wongchotigul; Dwip Kitayaporn; Mahidol UniversityMultiplex PCR amplification of lacZ, uidA and plc genes was developed for the simultaneous detection of total coliform bacteria for Escherichia coli and Clostridium perfringens, in drinking water. Detection by agarose gel electrophoresis yielded a... band of 876 bp for the lacZ gene of all coliform bacteria; a band of 147 bp for the uidA gene and a band of 876 bp for the lacZ gene of all strains of E. coli; a band of 280 bp for the plc gene for all strains of C. perfringens; and a negative resultPublication Open Access The removal of colors in non-carbonated soft drinks using granular activated carbon for coliform bacteria detection by sanitary indicator medium(2009) Vatanasomboon, P; Pisit Vatanasomboon; Rojanavipart, P; Piangchan Rojanavipart; Luksamijarulkul, P; Pipat Luksamijarulkul; พิศิษฐ์ วัฒนสมบูรณ์; เพียงจันทร์ โรจนวิภาต; พิพัฒน์ ลักษมีจรัลกุล; Srisantisang, N; Mahidol University. Faculty of Public Health. Department of Environmental Health Science; Mahidol University. Faculty of Public Health. Department of Biostatistics; Mahidol University. Faculty of Public Health. Department of MicrobiologyThis research was designed to study the efficiency of non-carbonated soft drink color removal by using granular activated carbon (GAC) for coliform bacteria detection by SI medium. The conditions established in the study were to use 6 color tones... for blue color-tone. The validity of coliform bacteria detection by SI medium for these removed-color soft drinks when compared with the standard MPN method were sensitivity value, specificity value and efficiency of test of more than 80%. Thus, GAC canPublication Metadata only Influence of enrichment broths on multiplex pcr detection of total coliform bacteria, escherichia coli and clostridium perfringens, in spiked water samples(2009-07-01) S. Worakhunpiset; P. Tharnpoophasiam; Mahidol UniversityAlthough multiplex PCR amplification condition for simultaneous detection of total coliform bacteria, Escherichia coli and Clostridium perfringens in water sample has been developed, results with high sensitivity are obtained when amplifyingPublication Open Access ปัจจัยที่เกี่ยวข้องและการปนเปื้อนแบคทีเรียชี้วัดในซูชิจากร้านจำหน่ายภายในอาคาร และร้านภายนอกอาคาร(2558) จิตตานันท์ ทองประเสริฐ; พิศิษฐ์ วัฒนสมบูรณ์; พิพัฒน์ ลักษมีจรัลกุล; ชัชวาล สิงหกันต์; Jittanun Thongprasert; Pisit Vatanasomboon; Pipat Luksamijarulkul; Chatchawal Singhakant; มหาวิทยาลัยมหิดล. คณะสาธารณสุขศาสตร์. ภาควิชาวิทยาศาสตร์อนามัยสิ่งแวดล้อม; มหาวิทยาลัยมหิดล. คณะสาธารณสุขศาสตร์. ภาควิชาจุลชีววิทยาอุณหภูมิภายในซูชิและค่าความเป็นกรด-ด่างของซูชิ รวมทั้งสำรวจสภาวะสุขาภิบาลอาหารทางกายภาพของร้านค้าโดยใช้แบบสำรวจ ตัวอย่างซูชิที่เก็บมาจะทำการวิเคราะห์หาปริมาณแบคทีเรียโคลิฟอร์มทั้งหมด(Total Coliform Bacteria) และฟีคัลโคลิฟอร์ม (Fecal Coliform Bacteria) ผลจาก... humidity, core temperature and pH value of sushi. The food sanitation condition of sushi shops were observed using an inspection form. The sushi samples were analyzed for the amount of total coliform and fecal coliform bacteria.The survey results showedPublication Open Access Bacterial Contamination in Raw Shucked Oysters in Shucking Houses and Retail Shops in Chon Buri Province,Thailand(2011) Chaweewun Intarakul; Suwanna Panutrakul; Sirichom Thungkao; ฉวีวรรณ อินทรกุล; สุวรรณา ภาณุตระกูล; ศิริโฉม ทุ่มเก้า; Burapha University. Faculty of Public Health; Burapha University. Faculty of Science. Department of Aquatic Science; Burapha University. Faculty of Science. Department of Microbiology; Mahidol University. Faculty of Science. Toxicology and Management of Chemicalstransformation of bacterial counts was conducted. The results revealed that counts of total bacteria, Staphylococcus aureus, and fecal coliforms in aseptically shucked oysters, were significantly lower than those same measurements in freshly-shucked oysters (p.... Fresh water exceeded the standards for total bacteria and coliforms in all samples, both before and after washing. The 40% of unwashed and 50% of washed shucked oysters exceeded the standard for total bacteria. The results indicated that bacterialPublication Metadata only Microbiological quality of drinking water and using water of a Chao Phya River community, Bangkok.(1994-12-01) P. Luksamijarulkul; V. Pumsuwan; S. Pungchitton; Mahidol Universityroutine testing of the bacteriological quality of drinking water is designed to detect the presence of coliform bacteria and virological assessment is to detect the presence of enteric viruses, especially hepatitis A virus (HAV). Therefore, this study... attempted to determine the HAV and coliform bacteria contamination in drinking water and using water of a Chao Phya River community, Bangkok where crowded living conditions increase the risk of water-related diseases. 95 samples of drinking water and 75Publication Metadata only Bacterial contamination of vegetables served in hospitals.(2005-12-01) Chertsak Dhiraputra; Chuntima Tiensasitorn; Wanida Techachaiwiwat; Naruemol Jirapanakorn; Kanchana Kachintorn; Somwang Danchaivijitr; Mahidol UniversityOBJECTIVES: To study bacterial contamination of fresh vegetables before cleaning and before serving to patients in 14 hospitals. MATERIAL AND METHOD: Aerobic plate count was performed and emphasized on total viable aerobic bacteria, fecal coliform... interviewed. RESULTS: One hundred and six of 403 of fresh vegetable samples (26.3%) before cleaning were contaminated with > 10(7) colony forming unit per gram (CFU/gram) of viable aerobic bacteria, 106 of 178 samples (59.6%) contained MPN/fecal coliform >1Publication Open Access การมีส่วนร่วมของประชาชนในชุมชนเศรษฐกิจพอเพียงศาลาดินในการจัดการคุณภาพน้ำในคลองมหาสวัสดิ์(2555-07) สุริยพงศ์ วัฒนาศักดิ์; Suriyapong Watanasak; รุจา ภู่ไพบูลย์; Rutja Phuphaibul; มหาวิทยาลัยมหิดล. คณะสิ่งแวดล้อมและทรัพยากรศาสตร์ทั้งหมด (TSS), ของแข็งทั้งหมดที่ละลายในน้ำÒ (TDS), ฟอสฟอรัสทั้งหมด (TP), ไนเตรท- ไนโตรเจน (NO3--N), แอมโมเนียไนโตรเจน (NH3-N), ออกซิเจนที่ละลายน้ำ (DO), total coliform bacteria, fecal coliform bacteria, แคดเมียม (Cd) และตะกั่ว (Pb) ผลการศึกษาที่สำคัญ...การบำบัดน้ำเสียให้มีประสิทธิภาพมากยิ่งขึ้น 2) ปัญหาน้ำÒเสียจาก โดยรอบชุมชน ซึ่งพบค่า fecal coliform bacteria ที่สูง เป็นการบ่งชี้ถึงการปนเปื้อนอุจจาระใน แหล่งน้ำÒ ดังนั้นโรงพยาบาลส่งเสริมสุขภาพตำบลมหาสวัสดิ์ร่วมกับชุมชนได้ดำเนินการกระตุ้น จิตสำนึกPublication Metadata only Assessment of a potential agricultural application of Bangkok-digested sewage sludge and finished compost products(2013-09-01) Siranee Sreesai; Panadda Peapueng; Taninporn Tippayamongkonkun; Suthipong Sthiannopkao; Mahidol University; Center of Excellence on Environmental Health and Toxicology; National Cheng Kung UniversityA study was conducted to investigate the levels of plant nutrients, heavy metals, parasites and fecal coliform bacteria in Bangkok-produced sewage sludge and finished compost products for potential agricultural application, as well as to compare... (1.16 ± 0.22%), but it was contaminated with human pathogens, including fecal coliform bacteria, viable helminthes egg and active forms of parasite cysts. Thus, fresh sewage sludge should not be disposed on land unless it has undergone pathogenPublication Metadata only A predictive model of the impact of urbanization on bacterial loads in watersheds(2021-05-15) Chantima Piyapong; Nitcha Chamroensaksri; Sayam Aroonsrimorakot; Lawan Eyosawat; Surasak Khankhum; Sunirat Rattana; Nuchsupha Sunthamala; Panya Warapetcharayut; Emmanuel Paradis; Mahidol University; Burapha University; CNRS Centre National de la Recherche Scientifique; Thailand National Science and Technology Development Agency; Mahasarakham University; Ministry of Natural Resources and Environment; Ministry of Natural Resources and Environment; Ministry of Natural Resources and Environmentin eight watersheds in Eastern Thailand to quantify the temporal and geographical variation in total and fecal coliform bacteria. Descriptive statistics showed considerable seasonal, inter-annual, and geographical variation. In order to quantify this multi... on the bacterial concentrations. Urbanization was an important factor controlling concentrations of bacteria in rivers: we found that the proportion of urban area around a station had a statistically significant effect on log-transformed total coliform bacterial
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