Influence of enrichment broths on multiplex pcr detection of total coliform bacteria, escherichia coli and clostridium perfringens, in spiked water samples
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.40, No.4 (2009), 795-800
Suggested Citation
S. Worakhunpiset, P. Tharnpoophasiam Influence of enrichment broths on multiplex pcr detection of total coliform bacteria, escherichia coli and clostridium perfringens, in spiked water samples. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.40, No.4 (2009), 795-800. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28013
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Influence of enrichment broths on multiplex pcr detection of total coliform bacteria, escherichia coli and clostridium perfringens, in spiked water samples
Although multiplex PCR amplification condition for simultaneous detection of total coliform bacteria, Escherichia coli and Clostridium perfringens in water sample has been developed, results with high sensitivity are obtained when amplifying purified DNA, but the sensitivity is low when applied to spiked water samples. An enrichment broth culture prior PCR analysis increases sensitivity of the test but the specific nature of enrichment broth can affect the PCR results. Three enrichment broths, lactose broth, reinforced clostridial medium and fluid thioglycollate broth, were compared for their influence on sensitivity and on time required with multiplex PCR assay. Fluid thioglycollate broth was the most effective with shortest enrichment time and lowest detection limit.
