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Publication Metadata only Development of a microarray for identification of pathogenic Clostridium spp.(2010-02-01) Tavan Janvilisri; Joy Scaria; Robin Gleed; Susan Fubini; Michelle M. Bonkosky; Yrjö T. Gröhn; Yung Fu Chang; Cornell University; Oak Ridge Institute for Science and Education; Mahidol Universitytechniques for clostridia are laborious, are time consuming, and may adversely affect the therapeutic outcome. In this study, we developed an oligonucleotide diagnostic microarray for pathogenic Clostridium spp. The microarray specificity was tested against... 65 Clostridium isolates. The applicability of this microarray in a clinical setting was assessed with the use of mock stool samples. The microarray was successful in discriminating at least 4 species with the limit of detection as low as 104CFU/mPublication Metadata only Reuse of bacterial artificial clones microarrays by stripping with sodium hydroxide(2018-05-01) Matchuporn Sukprasert; Chonthicha Satirapod; Worawan Lupthalug; Wicharn Choktanasiri; Kungpu Xu; Faculty of Medicine, Ramathibodi Hospital, Mahidol University; Weill Cornell Medical College, after stripping, the microarray can be reused for further experiments. Objective: To compare the accuracy in detected chromosomal aberrations between new and stripped slide by using NaOH/SCC as an array-stripping agent. Materials and Methods...: An experimental study was performed in an academic medical center. Two human known cell lines from fibroblast cell were used with whole genome amplification and microarray step according to the BlueGnome 24Sure protocol. Data processing was analyzed with BluefusePublication Metadata only Improved detection of nasopharyngeal cocolonization by multiple pneumococcal serotypes by use of latex agglutination or molecular serotyping by microarray(2011-05-01) Paul Turner; Jason Hinds; Claudia Turner; Auscharee Jankhot; Katherine Gould; Stephen D. Bentley; François Nosten; David Goldblatt; Shoklo Malaria Research Unit; Mahidol University; University of Oxford; St George's University of London; Wellcome Trust Sanger Institute; UCL Institute of Child Healthculture, were recultured in duplicate. A sweep of colonies from one plate culture was serotyped by latex agglutination. DNA extracted from the second plate was analyzed by S. pneumoniae molecular-serotyping microarray. Multiple serotypes were detected... in 11.2% of the swabs by WHO culture, 43.2% by sweep serotyping, and 48.8% by microarray. Sweep and microarray were more likely to detect multiple serotypes than WHO culture (P < 0.0001). Cocolonization detection rates were similar between microarrayPublication Metadata only Application of carbohydrate microarray technology for the detection of Burkholderia pseudomallei, Bacillus anthracis and Francisella tularensis antibodies(2008-11-03) N. Parthasarathy; R. Saksena; P. Kováč; D. DeShazer; S. J. Peacock; V. Wuthiekanun; H. S. Heine; A. M. Friedlander; C. K. Cote; S. L. Welkos; J. J. Adamovicz; S. Bavari; D. M. Waag; U.S. Army Medical Research Institute of Infectious Diseases; National Institute of Diabetes and Digestive and Kidney Diseases; Mahidol UniversityWe developed a microarray platform by immobilizing bacterial 'signature' carbohydrates onto epoxide modified glass slides. The carbohydrate microarray platform was probed with sera from non-melioidosis and melioidosis (Burkholderia pseudomallei...) individuals. The platform was also probed with sera from rabbits vaccinated with Bacillus anthracis spores and Francisella tularensis bacteria. By employing this microarray platform, we were able to detect and differentiate B. pseudomallei, B. anthracis and FPublication Metadata only Fabrication of protein microarrays for alpha fetoprotein detection by using a rapid photo-immobilization process(2016-03-01) Sirasa Yodmongkol; Boonsong Sutapun; Verayuth Praphanphoj; Toemsak Srikhirin; Thomas Brandstetter; Jürgen Rühe; Mahidol University; Suranaree University of Technology; Rajanukul Institute; Universität Freiburg im Breisgau; Medical Genetics Center© 2016 The Authors. In this study, protein microarrays based on sandwich immunoassays are generated to quantify the amount of alpha fetoprotein (AFP) in blood serum. For chip generation a mixture of capture antibody and a photoactive copolymer... consisting of N,N-dimethylacrylamide (DMAA), methacryloyloxy benzophenone (MaBP), and Na-4-styrenesulfonate (SSNa) was spotted onto unmodified polymethyl methacrylate (PMMA) substrates. Subsequently to printing of the microarray, the polymer and protein werePublication Metadata only Application of polysaccharide microarray technology for the serodiagnosis of Burkholderia pseudomallei infection (melioidosis) in humans(2008-01-01) Narayanan Parthasarathy; David DeShazer; Sharon J. Peacock; Vanaporn Wuthiekanun; Marilyn J. England; Sarah L. Norris; David M. Waag; U.S. Army Medical Research Institute of Infectious Diseases; Mahidol Universityand O-antigen saccharides of lipopolysaccharide). A polysaccharide microarray platform was developed by immobilizing these polysaccharides onto glass slides. Employing this microarray, we were able to demonstrate the presence of antibodies... to these polysaccharide antigens in the sera of melioidosis patients, but not in serum from nonmelioidosis human subjects. The advantages of this polysaccharide microarray technology over the conventional tests for the serodiagnosis of melioidosis are discussed. CopyrightPublication Open Access FlexiChip package: an universal microarray with a dedicated analysis software for high-thoughput SNPs detection linked to anti-malarial drug resistance.(2009-10-15) Steenkeste, Nicolas; Dillies, Marie-Agnès; Khim, Nimol; Sismeiro, Odile; Chy, Sophy; Lim, Pharath; Crameri, Andreas; Bouchier, Christiane; Mercereau-Puijalon, Odile; Beck, Hans-Peter; Mallika Imwong; มัลลิกา อิ่มวงศ์; Dondorp, Arjen M.; Socheat, Duong; Rogier, Christophe; Coppée, Jean- Yves; Ariey, Frédéric; Steenkeste, Nicolas; Mahidol University. Faculty of Tropical Medicineof practical methods enabling high throughput analysis. Here a new Zip-code array is described, called FlexiChip, linked to a dedicated software program, which largely overcomes this problem. METHODS: Previously published microarray probes detecting single...-nucleotide polymorphisms (SNP) associated with parasite resistance to anti-malarial drugs (ResMalChip) were adapted for a universal microarray FlexiChip format. To evaluate the overall sensitivity of the FlexiChip package (microarray + software), the resultsPublication Metadata only Development of rapid qualitative detection DNA microarray platform for Salmonella typhimurium for food safety(2019-08-08) C. Samphaongern; C. Pipatpanukul; T. Srikhirin; Mahidol University; Burapha Universityto monitor bacterial contamination. However, the main drawback of this technique is its inherited time consumed during the culturing step, which requires about 72 hours. In this research, an alternative DNA microarray technique was developed for a qualitative... to promote the best hybridization. DNA microarray was fabricated by mixing candidate DNA probes with photoactive polymer network, (poly(DMAA-mABP-SSNa)), and was printed onto a plastic substrate by non-contact microspotter. DNA probe was covalentlyPublication Metadata only FlexiChip package: An universal microarray with a dedicated analysis software for high-thoughput SNPs detection linked to anti-malarial drug resistance(2009-11-30) Nicolas Steenkeste; Marie Agnès Dillies; Nimol Khim; Odile Sismeiro; Sophy Chy; Pharath Lim; Andreas Crameri; Christiane Bouchier; Odile Mercereau-Puijalon; Hans Peter Beck; Mallika Imwong; Arjen M. Dondorp; Duong Socheat; Christophe Rogier; Jean Yves Coppée; Frédéric Ariey; Institut Pasteur du Cambodge; Institut Pasteur, Paris; Amunix, Inc.; GENOPOLE; Swiss Tropical and Public Health Institute (Swiss TPH); Mahidol University; Centre for Clinical Vaccinology and Tropical Medicine; National Center for Parasitology, Entomology and Malaria Control; IMTSSA Institut de Medecine Tropicale du Service de Sante des Armeesof practical methods enabling high throughput analysis. Here a new Zip-code array is described, called FlexiChip, linked to a dedicated software program, which largely overcomes this problem. Methods. Previously published microarray probes detecting single...-nucleotide polymorphisms (SNP) associated with parasite resistance to anti-malarial drugs (ResMalChip) were adapted for a universal microarray FlexiChip format. To evaluate the overall sensitivity of the FlexiChip package (microarray + software), the resultsPublication Metadata only An integrated bioinformatics approach to the characterization of influenza A/H5N1 viral sequences by microarray data: Implication for monitoring H5N1 emerging strains and designing appropriate influenza vaccines(2010-12-01) Supaporn Kaewpongsri; Chonlaphat Sukasem; Chutatip Srichunrusami; Ekawat Pasomsub; Julien Zwang; Wantanit Pairoj; Wasun Chantratita; Mahidol University; Shoklo Malaria Research Unitpathogenic H5N1 viral isolations were collected from different areas of Thailand between 2003 and 2006, and were used for analysis of H5N1 genotypic testing with a semiconductor-based oligonucleotide microarray. All H5N1 samples and H1N1, H4N8 negative....89). Semi-conductor-based oligonucleotide array and oligonucleotide probes corresponded well when detecting H5N1. After fully correcting the subtype from the result of microarray signal intensity, the microarray output method combined with bioinformatics