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Publication Metadata only Phase transfer bioconversion of penicillin G into 6-APA by immobilized penicillin acylase in recycling aqueous two-phase systems with a light-sensitive copolymer(2007-06-01) Ke Ming Jin; Xue Jun Cao; Ying Ping Zhuang; Ju Chu; Si Liang Zhang; Duerasor Orawan; East China University of Science and Technology; Mahidol UniversityImmobilized penicillin acylase was used for bioconversion of penicillin G into 6-APA in aqueous two-phase systems consisted of a light-sensitive polymer (PNBC 300000) and Dextran 20000. Partition coefficients of 6-APA was found to be about 8... that of the control in the reaction of single aqueous phase buffer. Three repeated batches of reactions were carried out. In aqueous two-phase systems, substrate penicillin G, products 6-APA and phenylacetate acid are biased in top phase, while immobilized penicillinPublication Metadata only Pharmacokinetics of Rheumatic Fever Prophylaxis Regimens(1992-01-01) V. Thamlikitkul; S. Kobwanthanakun; S. Pruksachatvuthi; R. Lertluknithi; Mahidol UniversityThe pharmacokinetics of benzathine penicillin G (1200000 IU given intramuscularly), penicillin V (250 mg given orally twice daily), erythromycin stearate (250 mg given orally twice daily) and roxithromycin (150 mg given orally once daily) were... at intervals of up to 28 days after intramuscular injection and immediately before and 1–2 h after the fifth oral dose. The concentrations of penicillin G in all serum samples obtained on day 28 after parenteral benzathine penicillin G administration werePublication Metadata only Improved X-ray diffraction from Bacillus megaterium penicillin G acylase crystals through long cryosoaking dehydration(2011-12-01) Catleya Rojviriya; Thunyaluck Pratumrat; Mark A. Saper; Jirundon Yuvaniyama; Mahidol University; University Michigan Ann ArborPenicillin G acylase from Bacillus megaterium (BmPGA) is currently used in the pharmaceutical industry as an alternative to PGA from Escherichia coli (EcPGA) for the hydrolysis of penicillin G to produce 6-aminopenicillanic acid (6-APA), a penam... nucleus for semisynthetic penicillins. Despite the significant differences in amino-acid sequence between PGAs from Gram-positive and Gram-negative bacteria, a representative PGA structure of Gram-positive origin has never been reported. In this studyPublication Metadata only An open, randomized, controlled trial of penicillin, doxycycline, and cefotaxime for patients with severe leptospirosis(2004-11-15) Yupin Suputtamongkol; Kanigar Niwattayakul; Chuanpit Suttinont; Kitti Losuwanaluk; Roongroeng Limpaiboon; Wirongrong Chierakul; Vanaporn Wuthiekanun; Surapee Triengrim; Mongkol Chenchittikul; Nicholas J. White; Mahidol University; Muang Loei Ram Hospital; Maharaj Nakhon Ratchasima Hospital; Banmai Chaiyapod Hospital; Udon Thani Center Hospital; National Institutes of Health, Bethesda; Churchill Hospital, there is no consensus regarding the optimal treatment for severe leptospirosis. Methods. An open-label, randomized comparison of parenteral cefotaxime, penicillin G sodium (hereafter known as "penicillin G"), and doxycycline for the treatment of suspected severe... leptospirosis. A total of 132 patients had rickettsial infection diagnosed, and, for these patients, treatment with doxycycline was superior to treatment with penicillin G. Conclusions. Doxycycline or cefotaxime is a satisfactory alternative to penicillin GPublication Metadata only High expression of the penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its own pac minus mutant(2000-08-15) W. Panbangred; K. Weeradechapon; S. Udomvaraphant; K. Fujiyama; V. Meevootisom; Mahidol University; Osaka UniversityBy marker exchange mutagenesis, Bacillus megaterium strain UN-1 (Bm-UN1) was used to prepare a mutant strain B. megaterium UN-cat (Bm-UNcat) lacking the penicillin G acylase gene (pac). The pac gene from Bm-UN1 was subcloned into pTF6... and the resultant plasmid, pBA402, was introduced into Bm-UNcat and Bacillus subtilis. Bm-UNcat harbouring pBA402 produced high penicillin G acylase (PAC) activity of 13.7, 19.5 and 20.4 U ml1 at 24, 36 and 48 h of culture, respectively. This was two- to fivefoldPublication Metadata only Localization and characterization of inclusion bodies in recombinant Escherichia coli cells overproducing penicillin G acylase(1997-06-04) N. Sriubolmas; W. Panbangred; S. Sriurairatana; V. Meevootisom; Mahidol UniversityVarious concentrations of isopropyl β-D-thiogalactopyranoside (IPTG) were used to induce production of the enzyme penicillin G acylase by recombinant Escherichia coli harboring plasmid pQEA11. The plasmid pQEA11 carries a wild-type pga gene, whichPublication Metadata only Antimicrobial resistance to ceftazidime involving loss of penicillin-binding protein 3 in Burkholderia pseudomallei(2011-10-11) Narisara Chantratita; Drew A. Rholl; Bernice Sim; Vanaporn Wuthiekanun; Direk Limmathurotsakul; Premjit Amornchai; Aunchalee Thanwisai; Hui Hoon Chua; Wen Fong Ooi; Matthew T.G. Holden; Nicholas P. Day; Patrick Tan; Herbert P. Schweizer; Sharon J. Peacock; Mahidol University; Colorado State University; Genome Institute of Singapore; Wellcome Trust; Nuffield Department of Clinical Medicine; National University of Singapore; University of Cambridgewas due to deletion of a gene encoding a penicillin-binding protein 3 (BPSS1219) present within the region of genomic loss. The clinical ceftazidime-resistant variants failed to grow using commonly used laboratory culture media, including commercial bloodPublication Metadata only Risk factors for penicillin-resistant Streptococcus pneumoniae acquisition in patients in Bangkok(2000-12-01) Charungthai Dejthevaporn; Asda Vibhagool; Ammarin Thakkinstian; Sayomporn Sirinavin; Malai Vorachit; Mahidol University; Faculty of Medicine, Ramathibodi Hospital, Mahidol Universityto December 1997, treated at a teaching hospital in Bangkok. Penicillin susceptibility was determined by E-test and strains with MIC of >0.1μg/ml were considered resistant. Cases were the patients who had PRSP, and patients who had penicillin-susceptible...To identify risk factors for acquisition of penicillin-resistant Streptococcus pneumoniae (PRSP) in patients in Bangkok, using a case-control study, the study included patients with clinical specimens which grew S. pneumoniae during JanuaryPublication Metadata only Proteochemometric model for predicting the inhibition of penicillin-binding proteins(2015-01-01) Sunanta Nabu; Chanin Nantasenamat; Wiwat Owasirikul; Ratana Lawung; Chartchalerm Isarankura-Na-Ayudhya; Maris Lapins; Jarl E.S. Wikberg; Virapong Prachayasittikul; Mahidol University; Uppsala Universitetfor penicillin-G, cefixime and ceftriaxone with 50 PBP2 protein sequence data using partial least-squares projections to latent structures. The generated model revealed excellent predictability (R 2 = 0.91, Q 2 = 0.77, Q Ext penicillin-binding protein 2 (PBP2Publication Metadata only The interaction and transport of β-lactam antibiotics with the cloned rat renal organic anion transporter 1(1999-08-01) Surawat Jariyawat; Takashi Sekine; Michio Takeda; Nopporn Apiwattanakul; Yoshikatsu Kanai; Samaisukh Sophasan; Hitoshi Endou; Kyorin University School of Medicine; Mahidol Universitywas inhibited by all of the penicillins and cephalosporins tested. Penicillin G, carbenicillin, cephaloridine, cephalothin, cefazolin, and cephalexin inhibited [14C]PAH uptake via OAT1 in a competitive manner (K1 = 0.29-2.33 mM). Cinoxacin, a quinolone gyrase... inhibitor, also inhibited PAH uptake via OAT1. Other antibiotics, such as gentamicin, streptomycin, and vancomycin, which do not contain anionic moieties, did not interact with OAT1. [3H]Penicillin G and [14C]cephaloridine were demonstrated to be transported