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Publication Open Access จากพื้นฐานสู่ความก้าวหน้าของการรักษาภาวะมีบุตรยาก(2560) ชลธิชา สถิระพจน์; วิชาญ โชคธนะศิริ; Chonthicha Satirapod; Wicharn Choktanasiri; มหาวิทยาลัยมหิดล. คณะแพทยศาสตร์โรงพยาบาลรามาธิบดี. ภาควิชาสูติศาสตร์-นรีเวชวิทยาreproductive technology is used to assist in the treatment of infertility and to increase the rate of pregnancy, including In-Vitro Fertilization (IVF), Intra-cytoplasmic Sperm Injection (ICSI). Moreover the assisted reproductive technology has led.... The success rate for treating infertility depends on the cause of infertility. Therefore, it is important to prioritize the assessment of spouses before treatment to find out the cause of infertility completely female and male. Currently, the assistedPublication Open Access Birth of kittens after the transfer of frozen-thawed embryos produced by intracytoplasmic sperm injection with spermatozoa collected from cryopreserved testicular tissue.(2012) T Tharasanit; S Buarpung; S Manee-In; C Thongkittidilok; N Tiptanavattana; P Comizzoli; M Techakumphu; Mahidol University. Faculty of Veterinary Science. Department of Clinical Science and Public HealthThe aim of this study is to produce live kittens from oocytes fertilized by intracytoplasmic sperm injection (ICSI) with frozen/thawed testicular spermatozoa. Spermatozoa were collected from thawed testicular tissue and subsequently injected into in vitro matured cat oocytes. At 24 h post-ICSI, presumptive zygotes/cleaved embryos were treated with 10 μm forskolin for 24 h to reduce intracellular lipid content of embryos (delipidation). At 48 h after oocyte injection, cleaved embryos (2- to 8-cell stage) were frozen in 10% (v/v) ethylene glycol-based medium by a slow controlled rate method and stored in liquid nitrogen. To evaluate in vitro and in vivo developmental competence, frozen embryos were thawed and then cultured for 6 days (n = 155) or cultured for 2 h before transferred (n = 209) to hormonal (equine chorionic gonadotropin/hCG)-treated cat recipients. Cleavage frequency at day 2 after ICSI with frozen/thawed testicular spermatozoa was ~30%. The percentages of frozen/thawed embryos that developed to morula and blastocyst stage (on day 3 and day 6 of in vitro culture, respectively) were significantly lower than that of fresh ICSI embryos (22.6 vs 45.2% and 21.3 vs 38.7%, respectively; p < 0.05). However, no difference was found in the number of blastomeres between frozen/thawed (242.5 ± 43.1) and fresh (320.2 ± 28.1) blastocysts. Three of seven cat recipients were pregnant and one pregnant cat delivered two healthy kittens. This is the first report of the birth of kittens after the transfer of frozen-thawed embryos produced by ICSI with frozen/thawed testicular sperm.Publication Open Access Changes in Sperm Quality After Two Different Techniques of Sperm Preparation(2012) Pattraporn Satitsuksanoa; Somsak Suthuvoravut; Anna Wongkularb; ภัทราภรณ์ สถิตย์สุขเสนาะ; สมศักดิ์ สุทัศน์วรวุฒิ; แอนนา วงษ์กุหลาบ; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of Obstetrics and GynecologyIntroduction: Sperm preparation is an important step in Assisted Reproductive Technology (ART). The two commonly used sperm preparation techniques in ART laboratory are the swim-up technique and density gradient centrifugation. The objectiveItem Open Access Annual Report 2013 กองบริหารงานวิจัย มหาวิทยาลัยมหิดล(2014) มหาวิทยาลัยมหิดล. สำนักงานอธิการบดี. กองบริหารงานวิจัยPublication Open Access Effects of vitrification on nuclear maturation, ultrastructural changes and gene expression of canine oocytes(2010) Bongkoch Turathum; Kulnasan Saikhun; Parisatcha Sangsuwan; Yindee Kitiyanant; Mahidol University. Institute of Molecular Biosciences; Mahidol University. Faculty of Science. Department of AnatomyBackground Cryopreservation of oocytes, which is an interesting procedure to conserve female gametes, is an essential part of reproductive biotechnology. The objective of the present study was to investigate the effects of vitrification on nuclearItem Open Access Annual Report 2012 กองบริหารงานวิจัย มหาวิทยาลัยมหิดล(2013) มหาวิทยาลัยมหิดล. สำนักงานอธิการบดี. กองบริหารงานวิจัยPublication Open Access Multidimensional indicators of healthy women in reproductive age(2008) Boonthum, A; Angsana Boonthum; Theerawanviwat, D; อังสนา บุญธรรมThe purposes of this study are to describe indicators of healthy women at reproductive age in each dimension affecting their healthy condition and to examine the internal structure of various dimensions of a reproductive age construct. A survey... group of six dimensions: physical health, mental health, social health, health-promoting behavior, health risk behavior and reproductive health. Structural equation modeling was employed to examine the theoretical models of the Healthy WomenPublication Open Access The importance of epigenetics in embryonic development and reproductive biotechnology(2012) Tuempong Wongtawan; Mahidol University. Faculty of Veterinary Science. Department of Pre-clinic and Applied Animal Scienceimportant step during mouse development is the establishment of epigenetic reprogramming which is believed to be vital for normal growth and development, however; the mechanism is still poorly understood. Creating embryo using reproductive biotechnology... such as IVM/IVF, ICSI and nuclear transfer may cause abnormal epigenetic pattern. This review summarise the role and the importance of epigenetic in embryonic development as well as reproductive biotechnology from research literature mainly in mouse used as aPublication Open Access The importance of epigenetics in embryonic development and reproductive biotechnology(2012) Tuempong Wongtawan; Mahidol University. Faculty of Veterinary Science. Department of Pre-clinic and Applied Animal Scienceimportant step during mouse development is the establishment of epigenetic reprogramming which is believed to be vital for normal growth and development, however; the mechanism is still poorly understood. Creating embryo using reproductive biotechnology... such as IVM/IVF, ICSI and nuclear transfer may cause abnormal epigenetic pattern. This review summarise the role and the importance of epigenetic in embryonic development as well as reproductive biotechnology from research literature mainly in mouse usedItem Open Access Annual Report 2008 College of Management Mahidol University(2009) Mahidol University. College of Management