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Item Open Access Identification of peptides for immune activation and responses in Thai patients with systemic sclerosis(Mahidol University, 2020) Oranit Likhit; Kobporn Boonnak; Sa-nga Pattanakitsakul; Watip TangjittipokinSystemic sclerosis (SSc), or scleroderma, is a chronic autoimmune disease involving a multisystem disorder characterized by skin and visceral organ fibrosis (such as heart, lungs, kidneys, gastrointestinal tract, and musculoskeletal systems.... The etiology of SSc remains unknown but genetics, environmental factors, and immunological abnormalities have been found to contribute to the disease development. This study aims to investigate the role of CD4 + and CD8 + T cells on pathogenesis of systemicPublication Open Access Natural Regulatory T cells (nTreg) in Minimal Change Disease and IgM Nephropathy(2012) Yada Louischaroen; Masatha Thongpan; Sansanee C. Chaiyaroj; Prasit Keesukphan; Panas Chalermsanyakorn; Vasant Sumethkul; Chagriya Kitiyakara; ญาดา หลุยเจริญ; มษฐา ทองปาน; ศันสนีย์ ไชยโรจน์; ประสิทธิ์ กี่สุขพันธ์; พนัส เฉลิมแสนยากร; วสันต์ สุเมธกุล; ชาครีย์ กิติยากร; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of Medicine; Mahidol University. Faculty of Science; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of Family Medicine; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of Pathologyimplicated in a number of autoimmune diseases. Objective: This is a pilot study to examine the number of Foxp3+CD4+ T cells in patients with MCD or IgM nephropathy. Methods: Eleven patients (2 patients with MDC and 9 patients with IgM nephropathy) and 25...Background: IgM nephropathy is a variant of minimal change disease (MCD) and is the commonest cause of nephrotic syndrome among Thai adults. Although patients with IgM nephropathy generally respond well to treatment with corticosteroids, patientsPublication Open Access The efficacy of edaravone (radicut), a free radical scavenger, for cardiovascular disease.(2013-07) Kikuchi, Kiyoshi; Salunya Tancharoen; ศรัณยา ตันเจริญ; Takeshige, Nobuyuki; Yoshitomi, Munetake; Morioka, Motohiro; Murai, Yoshinaka; Tanaka, Eiichiro; Mahidol University. Faculty of Dentistry. Department of PharmacologyEdaravone was originally developed as a potent free radical scavenger, and has been widely used to treat acute ischemic stroke in Japan since 2001. Free radicals play an important role in the pathogenesis of a variety of diseases..., such as cardiovascular diseases and stroke. Therefore, free radicals may be targets for therapeutic intervention in these diseases. Edaravone shows protective effects on ischemic insults and inflammation in the heart, vessel, and brain in experimental studies. As wellPublication Open Access A clinicopathological correlation of the expression of the angiopoietin-Tie-2 receptor pathway in the brain of adults with Plasmodium falciparum malaria(2013) Panote Prapansilp; Isabelle Medana; Nguyen Thi Hoan Mai; Day, Nicholas PJ; Phu, Nguyen Hoan; Yeo, Tsin W; Hien, Tran Tinh; Nicholas J White; Anstey, Nicholas M; Turner, Gareth DH; Mahidol University. Faculty of Tropical Medicine. Mahidol-Oxford Tropical Medicine Research UnitBackground: Plasma angiopoietin (Ang)-2 is associated with disease severity and mortality in adults and children with falciparum malaria. However the mechanism of action of the angiopoietins in fatal malaria is unclear. This study aimedPublication Open Access Postmenopausal Osteoporosis in Relation to Toll-like Receptor-7 and HLA DQB1 Gene Expression on Peripheral Blood Mononuclear Cells(2016) Wanwisa Waiyaput; Mayuree Jirapinyo; Charnchai Suchartwattanachai; Boonsong Ongphiphadhanakul; Somphoch Pumipichet; Areepan Sophonsritsuk; วรรณวิสา ไวยพุฒ; มยุรี จิรภิญโญ; ชาญชัย สุชาติวัฒนชัย; บุญส่ง องค์พิพัฒนกุล; สมโภช ภูมิพิเชฐ; อารีย์พรรณ โสภณสฤษฎ์สุข; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Research, and Innovation; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of Obstetrics and Gynecology; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Department of MedicineBackground: Data from genome wide association study have demonstrated that the toll-like receptor-7 gene (TLR7), a well-known role in innate immunity, may participate in the development of postmenopausal osteoporosis. The HLA DQB1 gene is part of human leukocyte antigen (HLA) complex. HLA DQB1 plays a critical role in the immune system. Objective: To determine the association of postmenopausal osteoporosis and transcripts of TLR7 and HLA DQB1 from peripheral blood mononuclear cells (PBMCs). Methods: The study groups included 25 postmenopausal women with normal bone mineral density (BMD) and the other 25 women with osteoporosis. Blood was drawn from the subjects for bone markers and PBMCs preparation. RNA was then isolated and quantitative RT-PCR was performed for TLR7 and HLA DQB1 genes. Results: PBMCs expression of both TLR7 and HLA DQB1 were not significantly different between postmenopausal women with normal BMD and those with osteoporosis. (P = 0.567 and P = 0.248, respectively). Conclusions: It is unlikely that TLR7 and HLA DQB1 contribute a major role on postmenopausal osteoporosis in Thai women.Publication Open Access iNKT cells and their potential lipid ligands during viral infection.(2015-07-24) Anunya Opasawatchai; อนัญญา โอภาสวัตชัย; Ponpan Matangkasombut; Ponpan Matangkasombut; Mahidol University. Faculty of Dentistry; Mahidol University. Faculty of Science. Department of Microbiologybridging innate and adaptive immunity. Diseases ranging from allergy, autoimmunity, and cancer, as well as infectious diseases, including viral infection, have been reported to be influenced by iNKT cells. However, it remains unclear how iNKT cellsPublication Open Access Effect of simvastatin on monocyte chemoattractant protein-1 expression in endometriosis patients: a randomized controlled trial(2017) Wanwisa Waiyaput; Somphoch Pumipichet; Sawaek Weerakiet; Sasivimol Rattanasiri; Areepan Sophonsritsuk; Mahidol University. Department of Obstetrics & Gynecology, Ramathibodi Hospital . Reproductive Endocrinology and Infertility UnitBackground: Simvastatin is a promising new drug for the treatment of endometriosis. It is a cholesterol-lowering drug that acts by inhibiting HMG-CoA reductase, resulting in a decrease in mevalonate, a precursor of cholesterol and monocyte chemoattractant protein-1 (MCP-1). This study investigated the effect of pre-operative oral simvastatin administration on MCP-1 gene expression and serum MCP-1 protein levels in patients with endometriosis. Methods: A prospective, randomized, controlled study was conducted at the Reproductive Endocrinology Unit of the Department of Obstetrics and Gynecology at the Faculty of Medicine Ramathibodi Hospital. Forty women (mean age: 18–45 years) scheduled for laparoscopic surgery who had been diagnosed with endometriosis were recruited and randomly assigned to either a treatment group (20 mg/d of orally administered simvastatin for 2 weeks before surgery) or an untreated control group. Serum was collected before and after treatment and protein levels of MCP-1 were determined. MCP-1 and CD68 transcript levels were also quantified using real-time PCR on endometriotic cyst tissues. Results: MCP-1 gene expression on endometriotic cyst was not significantly different between the simvastatin-treated and untreated groups (P = 0.99). CD68 expression was higher in the treatment group compared to the control group, but this was not statistically significant (P = 0.055). Serum MCP-1 levels following simvastatin treatment were higher than in samples obtained before treatment (297.89 ± 70.77 and 255.51 ± 63.79 pg/ml, respectively) (P = 0.01). Conclusions: Treatment with 20 mg/d of simvastatin for 2 weeks did not reduce the expression of either the chemokine MCP-1 gene or macrophage-specific genes. Cumulatively, this suggests that simvastatin is not ideal for treating endometriosis because a higher dose of simvastatin (40–100 mg/d) would be needed to achieve the target outcome, which would significantly increase the risk of myopathy in patients. Trial registration: Thai Clinical Trials Registry TCTR20130627003 Registered: June 27, 2013.Publication Open Access Investigation of aromatase inhibitory activity of metal complexes of 8-hydroxyquinoline and uracil derivatives(2014-08-14) Veda Prachayasittikul; Ratchanok Pingaew; Virapong Prachayasittikul; Chanin Nantasenamat; Somsak Ruchirawat; Supaluk Prachayasittikul; Mahidol University. Faculty of Medical Technology. Department of Clinical Microbiology and Applied Technology; Mahidol University. Faculty of Medical Technology. Center of Data Mining and Biomedical InformaticsPurpose: Estrogens play important roles in the pathogenesis and progression of breast cancer as well as estrogen-related diseases. Aromatase is a key enzyme in the rate-limiting step of estrogen production, in which its inhibition is one strategy... for controlling estrogen levels to improve prognosis of estrogen-related cancers and diseases. Herein, a series of metal (Mn, Cu, and Ni) complexes of 8-hydroxyquinoline (8HQ) and uracil derivatives (4–9) were investigated for their aromatase inhibitoryPublication Open Access Increased alpha-defensin expression is associated with risk of coronary heart disease: a feasible predictive inflammatory biomarker of coronary heart disease in hyperlipidemia patients(2016) Yaowapa Maneerat; Kriengchai Prasongsukarn; Surachet Benjathummarak; Wilanee Dechkhajorn; Urai Chaisri; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathologyeffective intervention, atherosclerosis consequently causes coronary heart disease (CHD), which leads to increased risk of sudden death. Polymorphonuclear neutrophils play a pivotal role in inflammation and atherogenesis. Human neutrophil peptides (HNPsPublication Open Access Incidence of diabetes in Asian population and diabetic bone quality.(2015-05) Mahjabeen Hossain; Dutmanee Seriwatanachai; ดุษมณี เสรีวัฒนาชัย; Mahidol University. Faculty of Dentistry. Department of Oral Biologyand relative deficiency. World Health Organization (WHO) estimated the prevalence of diabetes for all age groups worldwide will be doubled in in 2030. Shockingly, the Bureau of Non Communicable Disease of Thailand found similar trend for Thai population