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Publication Metadata only Cellular mechanisms underlying the inhibitory effect of flufenamic acid on chloride secretion in human intestinal epithelial cells(2017-06-01) Pawin Pongkorpsakol; Chantapol Yimnual; Varanuj Chatsudthipong; Vatcharin Rukachaisirikul; Chatchai Muanprasat; Mahidol University; Prince of Songkla Universityvia mechanisms involving AMPK activation and NF-κB-suppression. The present study aimed to investigate the effect of FFA on transepithelial Cl− secretion in human intestinal epithelial (T84) cells. FFA inhibited cAMP-dependent Cl− secretion in T84 cell... monolayers with IC50 of ∼8 μM. Other fenamate drugs including tolfenamic acid, meclofenamic acid and mefenamic acid exhibited the same effect albeit with lower potency. FFA also inhibited activities of CFTR, a cAMP-activated apical Cl− channel, and KCNQ1/KCNEPublication Metadata only Duodenal calcium transporter mRNA expression in stressed male rats treated with diazepam, fluoxetine, reboxetine, or venlafaxine(2012-10-01) Narattaphol Charoenphandhu; Jarinthorn Teerapornpuntakit; Sarawut Lapmanee; Nateetip Krishnamra; Jantarima Charoenphandhu; Mahidol University1b ), but not the expression of TRPV5 or NCX1. The mRNA expressions of paracellular genes, ZO-1, occludin, and claudin-3, were not altered by restraint stress. Since several antidepressant or anxiolytic drugs effectively alleviate stress-induced...Chronic stress has been reported to decrease bone density and intestinal calcium absorption, but its underlying mechanism remains elusive. Since long-term exposure to glucocorticoids, major stress hormones from adrenal gland, is knownPublication Metadata only Aqueous extract of Abutilon indicum Sweet inhibits glucose absorption and stimulates insulin secretion in rodents(2009-08-01) Chutwadee Krisanapun; Penchom Peungvicha; Rungravi Temsiririrkkul; Yuvadee Wongkrajang; Mahidol UniversityThe objective of this study was to evaluate the antidiabetic effects of the aqueous extract derived from the Thai Abutilon indicum Sweet plant and to explore its effects on intestinal glucose absorption and insulin secretion. The authors... significant reduction in plasma glucose levels in 30 minutes after the administration in moderately diabetic rats, as compared with untreated rats (P < .05), and this was at a faster rate than the use of an antidiabetic drug, glibenclamide. The inhibitionItem Metadata only Evaluation of the Intestinal Permeability of Rosmarinic Acid from Thunbergia laurifolia Leaf Water Extract in a Caco-2 Cell Model(2022-06-01) Woottisin N.; Sukprasert S.; Kulsirirat T.; Tharavanij T.; Sathirakul K.; Mahidol UniversityThunbergia laurifolia (TL) has been traditionally used as an antidote and an antipyretic drug by folk healers for centuries in Thailand. Rosmarinic acid (RA) is major compound in TL extract and has attracted great interest due to its potential broad... pharmacological effects. Herein, the permeability of RA was investigated in TL extract and as a pure compound in a Caco-2 cell model by using high-performance liquid chromatography with a photodiode array detector (HPLC-PDA). The results reveal that the apparentPublication Metadata only Nematicidal effect of plumbagin on Caenorhabditis elegans: A model for testing a nematicidal drug(2016-05-01) Phantip Chaweeborisuit; Chinnawut Suriyonplengsaeng; Worawit Suphamungmee; Prasert Sobhon; Krai Meemon; Mahidol University; Burapha Universitydecreased the number of mitochondria in hypodermal and intestinal cells and body wall muscles and damaged the ultrastructure of these tissues. Taken together, plumbagin may be a new drug against parasitic nematodes...., Caenorhabditis elegans was used as a model for testing the anthelmintic effect of plumbagin. The compound exhibited a nematicidal effect against all stages of C. elegans: L4 was least susceptible, while L1 was most susceptible to plumbagin with an LC50of 220Publication Metadata only Identification of new small molecule inhibitors of cystic fibrosis transmembrane conductance regulator protein: In vitro and in vivo studies(2007-03-01) Chatchai Muanprasat; Santi Kaewmokul; Varanuj Chatsudthipong; Mahidol University-induced intestinal fluid secretion by 40%, whereas INH 2 produced no effect. Our results indicate that INH 1 could be a new class candidate for a blocker of cholera toxin-induced intestinal fluid secretion as well as a CFTR inhibitor. © 2007 Pharmaceutical...Cystic fibrosis transmembrane conductance regulator (CFTR) protein is a cAMP-regulated chloride channel that has been proposed as a pharmacological target to reduce intestinal fluid loss in cholera. The aim of this study was to identify new CFTRPublication Metadata only The Intestinal Efflux Transporter Inhibition Activity of Xanthones from Mangosteen Pericarp: An In Silico, In Vitro and Ex Vivo Approach(2020-12-11) Panudda Dechwongya; Songpol Limpisood; Nawong Boonnak; Supachoke Mangmool; Mariko Takeda-Morishita; Thitianan Kulsirirat; Pattarawit Rukthong; Korbtham Sathirakul; Mahidol University; Thaksin University; Kobe Gakuin University; Srinakharinwirot University(α-(44.68) and β-(46.08) MG versus the control (66.26); p < 0.05) indicating an inhibitory effect on Pgp activity. Test compounds additionally revealed a downregulation of MDR1 mRNA expression. Moreover, an ex vivo absorptive transport in everted... expression, which makes them possible candidates for reducing multidrug resistance. Additionally, they influence the bioavailability and transport of Pgp substrate drugs.Publication Metadata only Cytochrome P450 enzyme mediated herbal drug interactions (part 1)(2014-04-02) Sompon Wanwimolruk; Virapong Prachayasittikul; Mahidol University(PXR). As a PXR ligand, hyperforin promotes expression of CYP3A4 enzymes in the small intestine and liver. This in turn causes induction of CYP3A4 and can reduce the oral bioavailability of many drugs making them less effective. The available evidence...It is well recognized that herbal supplements or herbal medicines are now commonly used. As many patients taking prescription medications are concomitantly using herbal supplements, there is considerable risk for adverse herbal drug interactionsItem Metadata only Cannabinoid/lysophosphatidylinositol-sensing G-protein coupled receptor 55 promotes intestinal tight junction assembly and its mechanistic insights(2025-01-01) Treveeravoot S.; Sukmak P.; Chatkul P.; Arinno A.; Amonsiriwit S.; Supapol P.; Limwattananon T.; Choksukchalalai N.; Kitti-Udom N.; Chindaduangratn N.; Wachiradejkul W.; Satianrapapong W.; Kwanthongdee J.; Moonwiriyakit A.; Muanprasat C.; Pongkorpsakol P.; Treveeravoot S.; Mahidol UniversityIntestinal tight junction disruption initiates progression of related diseases including inflammatory bowel disease (IBD) with no FDA-approved drug for tight junction recovery. To demonstrate the effect of pharmacological activation... pathway permeability. In fact, O1602 treatment also prevented TNF-α-induced intestinal barrier disruption in IFN-γ-primed T84 and Caco-2BBe monolayers. The effect of O1602 treatment on enhancing TER across T84 cell monolayers was abolished by pre-treatmentPublication Metadata only Anti-proliferative effect of 8α-tigloyloxyhirsutinolide-13-O-acetate (8αTGH) isolated from Vernonia cinerea on oral squamous cell carcinoma through inhibition of STAT3 and STAT2 phosphorylation(2019-01-01) Phisit Pouyfung; Sirinthip Choonate; Ariyaphong Wongnoppavich; Pornpimol Rongnoparut; Kongthawat Chairatvit; Mahidol University; Chiang Mai Universitymedicine for treatment of intestinal diseases and various skin diseases in addition to possessing anti-cancer activity. However, the effect of 8α-tigloyloxyhirsutinolide-13-O-acetate (8αTGH) as a major sesquiterpene lactone compound found in V. cinerea...© 2018 Elsevier GmbH Background: The high mortality rate of oral cancers has stimulated the search for effective herbal medicines and their pharmacological targets. Vernonia cinerea, a perennial tropical herb, is wildly used as a traditional folk