12 results
Search Results
Now showing 1 - 10 of 12
Publication Metadata only Ionic dependence of the resting membrane potential of rabbit lacrimal gland in vitro(1977-08-01) Chumpol Pholpramool; Sutas Korpaibool; Mahidol Universitybicarbonate solution was -26.7 ± 0.3 mV, insidethe cell negative. 3. 3. Elevation of the external K + concentration ([K + ] 0 ) caused depolarization of the resting potential. When [K + ] 0 was above 5 mM, a tenfold increase in [K + ] 0 decreased the membranePublication Metadata only Evaluation of zero-length cross-linking procedure for immuno-magnetic separation of Leptospira(2011-01-01) Stefan Schreier; Galayanee Doungchawee; Sudarat Chadsuthi; Darapond Triampo; Wannapong Triampo; Mahidol University; South Carolina Commission on Higher Educationefficiency (CE). Main parameters that influence the conjugation procedure were optimized, including the amount of protein per milligram of magnetic particles, the pH and ionic strength of the conjugation buffer. The bead-bound leptospiral fraction... captured with approximately 50 μg of antibody-labelled particles. Strong particle agglutination could be observed during incubation for leptospiral concentrations in the range of 10 7 -10 8 cells per mL. Despite covalent binding, we show that the physicalPublication Metadata only Comparison of DNA, aminoethylglycyl PNA and pyrrolidinyl PNA as probes for detection of DNA hybridization using surface plasmon resonance technique(2010-01-15) Cheeraporn Ananthanawat; Tirayut Vilaivan; Voravee P. Hoven; Xiaodi Su; Chulalongkorn University; Mahidol University; Institute of Materials Research and Engineering, A-Star, Singaporeof SPR on various important aspects. These include the effect of ionic strength on hybridization efficiency, the specificity to detect the mismatch(es) in target DNAs, the direction of binding (parallel or antiparallel) to target DNAs, and the effect... of target DNA concentration on hybridization efficiency. Results indicated that the immobilized acpcPNA probe possesses distinct hybridization properties relative to aegPNA (and/or DNA) counterparts, including a higher single-base mismatch sensitivityPublication Metadata only Observation of salt-induced β-lactoglobulin aggregation using sedimentation field-flow fractionation(2006-11-01) Sudarat Saeseaw; Juwadee Shiowatana; Atitaya Siripinyanond; Mahidol University-lactoglobulin concentrations, as well as contact time, on the aggregation of β-lactoglobulin were examined. All factors exhibited a combined effect on the size of aggregates, whereby larger aggregates were obtained at increased concentrations of Zn2+and β...-lactoglobulin. At fixed concentrations of 2% (w/v) β-lactoglobulin and 10 mM Zn2+, the particle size of the aggregates increased from 0.19 μm (at 15 min) to 0.38 μm (at 2880 min). Further, a hyphenated technique of SdFFF and inductively coupled plasma-opticalPublication Metadata only Characterization of drug-chitosan interaction by 1H NMR, FTIR and isothermal titration calorimetry(2008-05-01) Yaowalak Boonsongrit; Bernd W. Mueller; Ampol Mitrevej; Mahidol University; Christian-Albrechts-Universitat zu KielElectrostatic interaction between opposite charge of drugs (insulin and benzoic acid) and chitosan was studied by 1H NMR, FTIR and isothermal titration calorimetry (ITC). No ionic interaction between the carboxyl group of benzoic acid and the amine... group of chitosan could be detected. There was a minor change in the FTIR spectra of insulin-chitosan microparticles made of different concentrations of insulin. Exothermic heat of reaction between insulin and chitosan was obtained by ITC. HoweverPublication Metadata only Clindamycin hydrochloride-loaded composite hydrogel of poly((ethylene glycol) dimethacrylate-glycidyl methacrylate) and mesoporous silica nanoparticles for bacterial infection treatment(2020-01-01) Piyarat Sungkhaphan; Boonlom Thavornyutikarn; Pakkanun Kaewkong; Weerachai Singhatanadgit; Soraya Pornsuwan; Wanida Janvikul; Thailand National Metal and Materials Technology Center; Mahidol University; Thammasat Universityinto and released from the specimen, when water was used as drug loading medium. The contents of CDM loaded and released were, however, considerably raised as a function of ion concentration of SBF used, indicating the ionic interactions among CDM, MSNs, and SBF.... Then, individual hydrogels were immersed in 0.5 mL of aqueous clindamycin hydrochloride (CDM, 20 mg/mL), mixed with or without simulated body fluid (SBF) used at different ion concentrations, at 37oC for 3 days. The amounts of CDM loaded into and released from eachItem Metadata only Stability and biological activity enhancement of fucoxanthin through encapsulation in alginate/chitosan nanoparticles(2024-04-01) Sorasitthiyanukarn F.N.; Muangnoi C.; Rojsitthisak P.; Rojsitthisak P.; Sorasitthiyanukarn F.N.; Mahidol Universityemulsification and ionic gelation. The optimal formulation consisted of an ALG:CS mass ratio of 0.015:1, 0.71 % w/v Tween™ 80, and 5 mg/mL FX concentrations. The resulting FX-ALG/CS-NPs had a size of 227 ± 23 nm, a zeta potential of 35.3 ± 1.7 mPublication Metadata only Adjustment of medium composition and iso-osmotic potential in direct-shoot organogenesis produces true-to-type oil palm (Elaeis guineensis Jacq.) plantlets(2017-12-01) Kanokwan Romyanon; Kamolmanus Watana; Aussanee Pichakum; Kriengkrai Mosaleeyanon; Chalermpol Kirdmanee; Thailand National Science and Technology Development Agency; Mahidol University. In this study, we tested the effects of medium composition using three basal media types (Murashige and Skoog (MS), woody plant medium (WPM), and Y3) with concentrations of sucrose and different ionic strengths derived from sucrose, sorbitol, or both sucroseItem Metadata only Physical properties and in-vitro gastrointestinal digestion of oil-in-water emulsions stabilized by single- and dual-modified cassava starches with cross-linking and octenylsuccinylation(2024-03-01) Sriprablom J.; Winuprasith T.; Suphantharika M.; Wongsagonsup R.; Sriprablom J.; Mahidol Universityemulsions (oil content 10% (w/w)) at a concentration of 4.5% (w/w) compared to native cassava starch (NCS) and their physical properties and in-vitro gastrointestinal digestion were investigated. The emulsions stabilized with NCS and 2%CL-MCS had larger oil... gastrointestinal tract, all 3%OS-MCS promoted droplet flocculation, while the less ionic NCS and the 2%CL-MCS showed a decrease in droplet size after passing through the mouth and stomach stages. The lipid digestion rate of emulsions stabilized with different MCSPublication Metadata only Potentiality of melittin-loaded niosomal vesicles against vancomycin-intermediate staphylococcus aureus and staphylococcal skin infection(2021-01-01) Sirikwan Sangboonruang; Natthawat Semakul; Mohammad A. Obeid; Marta Ruano; Kuntida Kitidee; Usanee Anukool; Kidsadagon Pringproa; Panuwan Chantawannakul; Valerie A. Ferro; Yingmanee Tragoolpua; Khajornsak Tragoolpua; Yarmouk University; University of Strathclyde; Mahidol University; Chiang Mai University-intermediate S. aureus (VISA) was investigated by minimum inhibitory concentration (MIC) and time-killing assays. The localization of Mel on the bacterial cell was visualized by confocal laser scanning microscopy and its effect on the membrane was indicated... based on propidium iodide uptake. The non-ionic surfactant vesicle (NISV) or niosome nanocarrier was established for Mel loading (Mel-loaded NISV) by the thin-film hydration method. Physicochemical and in vitro biological properties of Mel-loaded NISVs