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Publication Metadata only Advances in Adoptive Cell Therapy Using Induced Pluripotent Stem Cell-Derived T Cells(2021-09-28) Ratchapong Netsrithong; Methichit Wattanapanitch; Siriraj HospitalAdoptive cell therapy (ACT) using chimeric antigen receptor (CAR) T cells holds impressive clinical outcomes especially in patients who are refractory to other kinds of therapy. However, many challenges hinder its clinical applications. For example..., patients who undergo chemotherapy usually have an insufficient number of autologous T cells due to lymphopenia. Long-term ex vivo expansion can result in T cell exhaustion, which reduces the effector function. There is also a batch-to-batch variation duringPublication Metadata only Internalization of the dengue virus is cell cycle modulated in HepG2, but not Vero cells(2004-11-01) Waranyoo Phoolcharoen; Duncan R. Smith; Mahidol UniversityWhile many studies have investigated the relationship between cell type and dengue virus infection, no study to date has examined the effect of cell physiology on permissiveness to infection. Unsynchronized and artificially synchronized cell... populations at different stages of the cell cycle of two cell types (Vero and HepG2) were examined for permissiveness to infection by two dengue virus serotypes (serotypes 2 and 3) by determining both the levels of virus produced as well as the percentagePublication Metadata only Memory B cells: A proposed new immunological correlate for protective efficacy of Japanese encephalitis vaccine(2013-08-01) Eiji Konishi; Mahidol Universityneutralizing antibodies and titers of 1:10 or higher are the recommended immunological correlate of protection. In this paper, the authors demonstrated, using a highly susceptible knockout mouse model, that memory B cells are required for disease protection... and that detectable neutralizing antibodies at the time of challenge are dispensable. The authors proposes that the extent of memory B cells would be an alternative and better immunological correlate for evaluating the efficacy of JE vaccine candidates in clinicalPublication Metadata only iNKT cells and their potential lipid ligands during viral infection(2015-01-01) Anunya Opasawatchai; Ponpan Matangkasombut; Mahidol Universityand promote immunological crosstalk thus bridging innate and adaptive immunity. Diseases ranging from allergy, autoimmunity, and cancer, as well as infectious diseases, including viral infection, have been reported to be influenced by iNKT cells. However...© 2015 Opasawatchai and Matangkasombut. Invariant natural killer T (iNKT) cells are a unique population of lipid-reactive CD1d-restricted innate-like T lymphocytes. Despite being a minor population, they serve as an early source of cytokinesItem Metadata only Outline-based geometric morphometrics: Wing cell differences for mosquito vector classification in the Tanaosri mountain range, Thailand(2024-02-01) Laojun S.; Changbunjong T.; Sumruayphol S.; Chaiphongpachara T.; Laojun S.; Mahidol UniversityRecent studies have revealed taxonomic signals within the wing cells of certain mosquito species. In our study, wing cell differentiation among mosquito vectors from the Tanaosri mountain range in Thailand was evaluated using the outline-based... geometric morphometric (GM) approach. Our focus was on four specific wing cells for GM analysis: the wing contour (external cell), the second submarginal cell (internal cell 1), the first posterior cell (internal cell 2), and the third posterior cellPublication Metadata only Determination of cell expansion and surface molecule expression on anti-CD3/28 expanded CD4+ T cells(2019-11-01) Premrutai Thitilertdecha; Poonsin Poungpairoj; Varangkana Tantithavorn; Palanee Ammaranond; Nattawat Onlamoon; Chulalongkorn University; Faculty of Medicine, Siriraj Hospital, Mahidol University© 2019 The Scandinavian Foundation for Immunology CD4+ T cell immunotherapy has potential for treatment in HIV-infected patients. A large number of expanded CD4+ T cells and confirmation of functional-related phenotypes are required for ensuring... the successful outcomes of treatment. Freshly isolated CD4+ T cells from healthy donors were activated with anti-CD3/28-coated magnetic beads at different bead-to-cell ratios and cultured in the absence and presence of IL-2 supplementation for 3 weeks. FoldPublication Metadata only Crosstalk between Tumor-Infiltrating Immune Cells and Cancer-Associated Fibroblasts in Tumor Growth and Immunosuppression of Breast Cancer(2021-01-01) Jarupa Soongsathitanon; Pranisa Jamjuntra; Nuttavut Sumransub; Supaporn Yangngam; Marjorie De La Fuente; Glauben Landskron; Peti Thuwajit; Marcela A. Hermoso; Chanitra Thuwajit; Siriraj Hospital; Clinica Las Condes; Facultad de Medicina de la Universidad de ChileSignals from the tumor microenvironment (TME) have a profound influence on the maintenance and progression of cancers. Chronic inflammation and the infiltration of immune cells in breast cancer (BC) have been strongly associated with early... carcinogenic events and a switch to a more immunosuppressive response. Cancer-associated fibroblasts (CAFs) are the most abundant stromal component and can modulate tumor progression according to their secretomes. The immune cells including tumor-infiltratingItem Metadata only N-acetylcysteine potentiates the tumor cytotoxicity of cytokine-induced killer cells(2025-09-01) Ek-Eudomsuk P.; Chalermrujinanant C.; Soontrapa K.; Ek-Eudomsuk P.; Mahidol UniversityBackground: Cytokine-induced killer (CIK) cells are an ex vivo expanded heterogeneous population of natural killer (NK)-like T cells that can exert potent MHC-unrestricted antitumor activity. A number of pre-clinical and clinical studies have... demonstrated that CIK cells can serve as a safe and potent immunotherapy of malignant tumors. N-acetylcysteine (NAC) has been demonstrated to enhance the T-cell functions by increasing their proliferation and cytokine production. Objective: To investigateItem Unknown The evolving role of mast cells in wound healing: insights from recent research and diverse models(2024-01-01) Guth C.; Limjunyawong N.; Pundir P.; Guth C.; Mahidol UniversityChronic wounds significantly burden health care systems worldwide, requiring novel strategies to ease their impact. Many physiological processes underlying wound healing are well studied but the role of mast cells remains controversial. Mast cells... are innate immune cells and play an essential role in barrier function by inducing inflammation to defend the host against chemical irritants and infections, among others. Many mast cell–derived mediators have proposed roles in wound healing; however, in vivoPublication Unknown Burkholderia pseudomallei induces cell fusion and actin-associated membrane protrusion: A possible mechanism for cell-to-cell spreading(2000-09-14) W. Kespichayawattana; S. Rattanachetkul; T. Wanun; P. Utaisincharoen; S. Sirisinha; Mahidol UniversityBurkholderia pseudomallei, a facultative intracellular bacterium, is the causative agent of a broad spectrum of diseases collectively known as melioidosis. Its ability to survive inside phagocytic and nonphagocytic cells and to induce multinucleated... giant cell (MNGC) formation has been demonstrated. This study was designed to assess a possible mechanism(s) leading to this cellular change, using virulent and nonvirulent strains of B. pseudomallei to infect both phagocytic and nonphagocytic cell lines