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Publication Metadata only Development of multiplex PCR for the detection of total coliform bacteria for Escherichia coli and Clostridium perfringens in drinking water(2005-01-01) Suwalee Tantawiwat; Unchalee Tansuphasiri; Waranya Wongwit; Varee Wongchotigul; Dwip Kitayaporn; Mahidol UniversityMultiplex PCR amplification of lacZ, uidA and plc genes was developed for the simultaneous detection of total coliform bacteria for Escherichia coli and Clostridium perfringens, in drinking water. Detection by agarose gel electrophoresis yielded a... band of 876 bp for the lacZ gene of all coliform bacteria; a band of 147 bp for the uidA gene and a band of 876 bp for the lacZ gene of all strains of E. coli; a band of 280 bp for the plc gene for all strains of C. perfringens; and a negative resultPublication Metadata only Influence of enrichment broths on multiplex pcr detection of total coliform bacteria, escherichia coli and clostridium perfringens, in spiked water samples(2009-07-01) S. Worakhunpiset; P. Tharnpoophasiam; Mahidol UniversityAlthough multiplex PCR amplification condition for simultaneous detection of total coliform bacteria, Escherichia coli and Clostridium perfringens in water sample has been developed, results with high sensitivity are obtained when amplifyingPublication Metadata only Microbiological quality of drinking water and using water of a Chao Phya River community, Bangkok.(1994-12-01) P. Luksamijarulkul; V. Pumsuwan; S. Pungchitton; Mahidol Universityroutine testing of the bacteriological quality of drinking water is designed to detect the presence of coliform bacteria and virological assessment is to detect the presence of enteric viruses, especially hepatitis A virus (HAV). Therefore, this study... attempted to determine the HAV and coliform bacteria contamination in drinking water and using water of a Chao Phya River community, Bangkok where crowded living conditions increase the risk of water-related diseases. 95 samples of drinking water and 75Publication Metadata only Bacterial contamination of vegetables served in hospitals.(2005-12-01) Chertsak Dhiraputra; Chuntima Tiensasitorn; Wanida Techachaiwiwat; Naruemol Jirapanakorn; Kanchana Kachintorn; Somwang Danchaivijitr; Mahidol UniversityOBJECTIVES: To study bacterial contamination of fresh vegetables before cleaning and before serving to patients in 14 hospitals. MATERIAL AND METHOD: Aerobic plate count was performed and emphasized on total viable aerobic bacteria, fecal coliform... interviewed. RESULTS: One hundred and six of 403 of fresh vegetable samples (26.3%) before cleaning were contaminated with > 10(7) colony forming unit per gram (CFU/gram) of viable aerobic bacteria, 106 of 178 samples (59.6%) contained MPN/fecal coliform >1Item Metadata only Assessment of water quality and associated health risks in community-managed water systems in highland areas: A case study Chiang Rai province, Thailand(2026-06-01) Sudsandee S.; Kliengchuay W.; Keawduanglek V.; Hongtong A.; Siriratruengsuk W.; Niampradit S.; Mingkhwan R.; Kiangkoo N.; Precha N.; Tantrakarnapa K.; Sudsandee S.; Mahidol UniversityAuthority (PWA)’s standard, including pH (41 %), iron (32 %), lead (5 %), turbidity (2 %), mercury (2 %), and lack of free residual chlorine (100 %). The water samples showed signs of biological contamination. Total coliform bacteria were found in 39... % of the samples, fecal coliform bacteria in 27 %, and Escherichia coli in 9 %. Health risk assessments indicated that hazard quotients (HQs) and hazard indices (HIs) for heavy metals were less than 1, suggesting a negligible health risk. Total coliform bacteriaPublication Metadata only Seasonal and Spatial Environmental Influence on Opisthorchis viverrini Intermediate Hosts, Abundance, and Distribution: Insights on Transmission Dynamics and Sustainable Control(2016-11-23) Christina Sunyoung Kim; Pierre Echaubard; Apiporn Suwannatrai; Sasithorn Kaewkes; Bruce A. Wilcox; Banchob Sripa; Khon Kaen University; Laurentian University; Mahidol University), Salinity, electrical conductivity (EC), total dissolved solid (TDS), nitrite nitrogen (NO2-N), lead (Pb), total coliform bacteria (TCB) and fecal coliform bacteria (FCB)] were measured. Multivariate analyses, linear models and kriging were usedPublication Metadata only Molecular characterization of antibiotic-resistant bacteria in contaminated chicken meat sold at supermarkets in Bangkok, Thailand(2012-11-28) Chaiyaporn Chaisatit; Chanwit Tribuddharat; Chaiwat Pulsrikarn; Surang Dejsirilert; Mahidol University; Thailand Ministry of Public Healthprobable number (MPN) analysis showed that 56.7% of the samples (34/60) were in violation of the limit of allowable coliform bacteria in chicken meat, for which the maximum is 46,000 MPN/g. Multidrug-resistant phenotypes of both S. enterica and E. coli were...SUMMARY: We assessed contamination by antibiotic-resistant bacteria in chicken meat obtained from supermarkets in Bangkok, Thailand. The prevalence of Salmonella enterica and Escherichia coli was 18.7% (14/75) and 53% (106/200), respectively. MostPublication Metadata only Drinking water investigation of hill tribes: A case study in northern thailand(2020-03-01) Suntorn Sudsandee; Krailak Fakkaew; Vivat Keawdounglek; Pussadee Laor; Suwalee Worakhunpiset; Tawatchai Apidechkul; Mae Fah Luang University; Mahidol Universitysettling before drinking water without treatment. 42.0% of the water samples had a turbidity values <5 NTU, and total coliform and fecal coliform bacteria were detected in 100.0% of the samples. To prevent water-borne diseases among the hill tribe peoplePublication Metadata only Bacterial contamination of bottle milk in infants under 6 months in children's hospital, Bangkok, Thailand(1999-12-01) Orasa Suthienkul; Kanokrat Siripanichgon; Panchit Promachot; Peter Echeverria; Udom Lexsomboon; Yasuyuki Rakue; Mahidol University; Bangkok Children's Hospital; Armed Forces Research Institute of Medical Sciences, Thailandfollowing interview of their caretakers after obtaining their consent. Bacterial contamination was found in 91.8% (459/500) of bottle milk samples. Among the positive samples, 82.8% (380/459) contained enteric bacteria, another 17.2% were unidentified... bacteria. The dominant enteric bacteria isolated from bottle milk were Klebsiella spp (56.6%), Enterobacter spp (41.3%), Aeromonas spp (14.4%), E. coli (13.4 %) and Vibrio cholerae non O-1 (1.8%). Isolated E. coli were further identified as enteropathogenicPublication Metadata only Water quality of the mun river in thailand—spatiotemporal variations and potential causes(2019-10-02) Haoyu Tian; Guo An Yu; Ling Tong; Renzhi Li; He Qing Huang; Arika Bridhikitti; Thayukorn Prabamroong; Institute of Geographical Sciences and Natural Resources Research Chinese Academy of Sciences; China Agricultural University; Mahidol University; Mahasarakham University(WQI) method. The Kendall test shows significant declines in fecal coliform bacteria (FCB) and ammonia (NH3) in the upper reaches and increases in nitrate (NO3) and NH3 in the lower reaches. Strong temporal and spatial fluctuations were observed in both