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Publication Metadata only Enzyme-linked immunosorbent assay for detection of antibody to Gnathostoma antigen in patients with intermittent cutaneous migratory swelling(1986-08-06) A. Dharmkrong-At; S. Migasena; P. Suntharasamai; D. Bunnag; R. Priwan; S. Sirisinha; Mahidol UniversityA sensitive enzyme-linked immunosorbent assay is described for the detection of immunoglobulin G antibody to Gnathostoma antigen in the sera of patients with intermittent cutaneous migratory swelling who were suspected to being infected... by the tissue nematode Gnathostoma spinigerum. The antigen used was a crude somatic aqueous extract of the third larval stage obtained from naturally infected eels. The 1:320 dilution of all sera from 46 patients gave enzyme-linked immunosorbent assay valuesPublication Metadata only Enzyme-linked immunosorbent assay for anti-streptococcal protoplast membrane antibodies(1984-11-01) N. Banchuin; J. Wheeler; M. Sussman; Mahidol University; Newcastle University, United KingdomAn enzyme-linked immunosorbent assay is described for the quantitative determination of circulating antibodies to streptococcal protoplast membranes (SPM) in human sera. The assay was used to study the prevalence of antibodies to SPM of M types 6Publication Metadata only Development of enzyme-linked immunosorbent assay for hepatitis B e antigen.(1990-03-01) B. Petchclai; S. Niemhom; Mahidol Universitydevelopment of enzyme-linked immunosorbent assay (ELISA) for HBeAg and its antibody (anti-HBe) was considered necessary and it was successfully conducted. The developed test was compared with ELISA test for HBeAg and anti-HBe manufactured by Organon TeknikaPublication Metadata only Evaluation of enzyme-linked immunosorbent assay (ELISA) test in the diagnosis of tuberculous meningitis.(1990-10-01) N. Poungvarin; A. Viriyavejakul; A. Leelarasamee; Mahidol UniversityThe detection of immunoglobulin G antibody in the CSF to purified protein derivative in patients with tuberculous meningitis and non-tuberculous meningitis patients by enzyme-linked immunosorbent assay (ELISA) were performed at the DepartmentPublication Metadata only Serodiagnosis of melioidosis by a competitive enzyme-linked immunosorbent assay using a lipopolysaccharide-specific monoclonal antibody(2005-06-01) Charin Thepthai; Saijai Smithtikarn; Montana Suksuwan; Sirirurg Songsivilai; Tararaj Dharakul; Mahidol University; Thailand Ministry of Public Healthcompetitive enzyme-linked immunosorbent assay (ELISA) using a lipopolysaccharide-specific monoclonal antibody was developed. The assay provides high specificity, based on a previously described monoclonal antibody to a specific epitope... on the lipopolysaccharide (LPS) of B. pseudomallei. The assay sensitivity of 96.0% and specificity of 100% were achieved at a cutoff value of 50% inhibition in human culture-proven melioidosis cases. An optimal cutoff value of 65% inhibition for sera from a melioidosisPublication Metadata only Immunoblotting and enzyme linked-immunosorbent assay for diagnosis of Toxoplasma infection in HIV Thai patients(1999-09-01) Sirichit Wongkamchai; Vanna Mahakittikun; Paron Dekumyoy; Jerawan Onrotchanakun; Faculty of Medicine, Siriraj Hospital, Mahidol University; Mahidol Universityin AIDS patients because of depressed antibody responses. Fifty serum samples were used in this study to investigate serological evidence of toxoplasmosis in HIV positive Thai patients by Platelia kit, the commercial enzyme-linked immunosorbent assayPublication Metadata only Rapid detection of Salmonella enterica serovar Choleraesuis in blood cultures by a dot blot enzyme-linked immunosorbent assay(2000-12-01) K. Janyapoon; S. Korbsrisate; H. Thamapa; S. Thongmin; S. Kanjanahareutai; N. Wongpredee; S. Sarasombath; Mahidol UniversityA dot blot enzyme-linked immunosorbent assay (ELISA) with a monoclonal antibody specific to phase1-c Salmonella was developed for the direct detection of Salmonella enterica serovar Choleraesuis in blood cultures. This system was applied.... In contrast, 77 samples infected with bacteria commonly isolated from the blood were not reactive by the ELISA. The calculated sensitivity and specificity of the established assay are 100%.Publication Metadata only Diagnosis of opisthorchiasis by enzyme-linked immunosorbent assay using partially purified antigens.(1990-01-01) N. Poopyruchpong; V. Viyanant; E. S. Upatham; P. Srivatanakul; Mahidol Universityby Sephadex G-200 gel filtration chromatography. Five fractions of O. viverrini antigens were obtained, namely tegument extract, somatic extract, fraction 1 (P 1 ), fraction 2 (P 2 ) and fraction 3 (P 3 ), respectively. The enzyme-linked immunosorbent assay... and 70 individuals from non-endemic areas infected with hookworm, Trichuris and Ascaris including 49 individuals with negative stool examination. The assays performed with tegument extract, somatic extract and P 1 fraction were found to have 100Publication Metadata only Enzyme-linked immunosorbent assay based on monoclonal and polyclonal antibodies for the detection of Entamoeba histolytica antigens in faecal specimens(1992-01-01) Ratri Wonsit; Nitaya Thammapalerd; Savanat Tharavanij; Prayong Radomyos; Danai Bunnag; Mahidol UniversityA murine monoclonal antibody (MAb) (Eh208C2-2) raised against crude lysate of the pathogenic HM- 1:IMSS strain of Entamoeba histolytica was used in an enzyme-linked immunosorbent assay for the detection of E. histolytica antigens in faecal specimens.... The detection limit of the assay was 110 and 280 amoebae/ml of the HM-1:IMSS and HK-9 strains in phosphate-buffered saline, respectively. The assay was applied to single stool samples from 3 groups of individuals comprising 40 patients whose stools were positivePublication Metadata only HIV-1 subtyping using gag/env heteroduplex mobility assay and peptide enzyme-linked immunosorbent assay(2005-06-01) Wantanee Simparak; Uraiwan Kositanont; Ruengpung Sutthent; Punneeporn Wasinrapee; Thanyanan Chaowanachan; Chantapong Wasi; Mahidol University; Centers for Disease Control (CDC), Thailand Field Stationyears. During 1997-1998, 227 blood samples were collected from HIV-1 infected individuals consisting of 92 mothers, 35 children and 100 IDUs. The blood samples were subtyped by heteroduplex mobility assay (HMA) and peptide enzyme-linked immunosorbent... assay (PEIA). Using gag and env HMA, CRF01Æ and subtype B′ accounted for 96-97% and 3-4% of both the mothers and the children, respectively. In the IDU group, 10% of the plasma samples could only be performed by gag HMA and gave the result as CRF01Æ. CRF