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Publication Metadata only Development of a microarray for identification of pathogenic Clostridium spp.(2010-02-01) Tavan Janvilisri; Joy Scaria; Robin Gleed; Susan Fubini; Michelle M. Bonkosky; Yrjö T. Gröhn; Yung Fu Chang; Cornell University; Oak Ridge Institute for Science and Education; Mahidol Universitytechniques for clostridia are laborious, are time consuming, and may adversely affect the therapeutic outcome. In this study, we developed an oligonucleotide diagnostic microarray for pathogenic Clostridium spp. The microarray specificity was tested against... 65 Clostridium isolates. The applicability of this microarray in a clinical setting was assessed with the use of mock stool samples. The microarray was successful in discriminating at least 4 species with the limit of detection as low as 104CFU/mPublication Metadata only Reuse of bacterial artificial clones microarrays by stripping with sodium hydroxide(2018-05-01) Matchuporn Sukprasert; Chonthicha Satirapod; Worawan Lupthalug; Wicharn Choktanasiri; Kungpu Xu; Faculty of Medicine, Ramathibodi Hospital, Mahidol University; Weill Cornell Medical College, after stripping, the microarray can be reused for further experiments. Objective: To compare the accuracy in detected chromosomal aberrations between new and stripped slide by using NaOH/SCC as an array-stripping agent. Materials and Methods...: An experimental study was performed in an academic medical center. Two human known cell lines from fibroblast cell were used with whole genome amplification and microarray step according to the BlueGnome 24Sure protocol. Data processing was analyzed with BluefusePublication Metadata only Improved detection of nasopharyngeal cocolonization by multiple pneumococcal serotypes by use of latex agglutination or molecular serotyping by microarray(2011-05-01) Paul Turner; Jason Hinds; Claudia Turner; Auscharee Jankhot; Katherine Gould; Stephen D. Bentley; François Nosten; David Goldblatt; Shoklo Malaria Research Unit; Mahidol University; University of Oxford; St George's University of London; Wellcome Trust Sanger Institute; UCL Institute of Child Healthculture, were recultured in duplicate. A sweep of colonies from one plate culture was serotyped by latex agglutination. DNA extracted from the second plate was analyzed by S. pneumoniae molecular-serotyping microarray. Multiple serotypes were detected... in 11.2% of the swabs by WHO culture, 43.2% by sweep serotyping, and 48.8% by microarray. Sweep and microarray were more likely to detect multiple serotypes than WHO culture (P < 0.0001). Cocolonization detection rates were similar between microarrayPublication Metadata only FlexiChip package: An universal microarray with a dedicated analysis software for high-thoughput SNPs detection linked to anti-malarial drug resistance(2009-11-30) Nicolas Steenkeste; Marie Agnès Dillies; Nimol Khim; Odile Sismeiro; Sophy Chy; Pharath Lim; Andreas Crameri; Christiane Bouchier; Odile Mercereau-Puijalon; Hans Peter Beck; Mallika Imwong; Arjen M. Dondorp; Duong Socheat; Christophe Rogier; Jean Yves Coppée; Frédéric Ariey; Institut Pasteur du Cambodge; Institut Pasteur, Paris; Amunix, Inc.; GENOPOLE; Swiss Tropical and Public Health Institute (Swiss TPH); Mahidol University; Centre for Clinical Vaccinology and Tropical Medicine; National Center for Parasitology, Entomology and Malaria Control; IMTSSA Institut de Medecine Tropicale du Service de Sante des Armeesof practical methods enabling high throughput analysis. Here a new Zip-code array is described, called FlexiChip, linked to a dedicated software program, which largely overcomes this problem. Methods. Previously published microarray probes detecting single...-nucleotide polymorphisms (SNP) associated with parasite resistance to anti-malarial drugs (ResMalChip) were adapted for a universal microarray FlexiChip format. To evaluate the overall sensitivity of the FlexiChip package (microarray + software), the resultsPublication Metadata only Cryptococcus gattii virulence composite: Candidate genes revealed by microarray analysis of high and less virulent Vancouver Island outbreak strains(2011-02-01) Popchai Ngamskulrungroj; Jennifer Price; Tania Sorrell; John R. Perfect; Wieland Meyer; The University of Sydney; Duke University School of Medicine; Mahidol Universityvirulent (R272), that were identified from the Vancouver outbreak. By expression microarray analys is, 202 genes showed at least a 2-fold difference in expression with 108 being up- and 94 being down-regulated in strain R265 compared with strain R272... in the regulation of mitosis and ergosterol biosynthesis were suppressed. In vitro phenotypic studies and transcription analysis confirmed the microarray results. Gene disruption of LAC1 and MPK1 revealed defects in melanin synthesis and cell wall integrityPublication Metadata only Human immune responses to Burkholderia pseudomallei characterized by protein microarray analysis(2011-04-01) Duangchan Suwannasaen; Jirawan Mahawantung; Wipada Chaowagul; Direk Limmathurotsakul; Philip L. Felgner; Huw Davies; Gregory J. Bancroft; Richard W. Titball; Ganjana Lertmemongkolchai; Khon Kaen University; Sappasitthiprasong Hospital; Mahidol University; University of California, Irvine; University of ExeterBackground. We aimed to determine the antibody and T cell responses to Burkholderia pseudomallei of humans to select candidate vaccine antigens. Methods. For antibody profiling, a protein microarray of 154 B. pseudomallei proteins was probed... patients. Conclusions. By combining large-scale antibody microarrays and assays of T cell-mediated immunity, we identified a panel of novel B. pseudomallei proteins that show distinct patterns of reactivity in different stages of human melioidosisItem Metadata only Application of Gene Expression Microarray for the Classification of Ph-Like B-Cell Acute Lymphoblastic Leukemia(2024-01-01) Thangrua N.; Siriboonpiputtana T.; Rerkamnuaychoke B.; Chareonsirisuthigul T.; Korkiatsakul V.; Pongphitcha P.; Mukda E.; Chutipongtanate S.; Pakakasama S.; Thangrua N.; Mahidol Universitypipeline for molecular characterization of the disease, and no valid predictor gene panel is available worldwide. Methods: We performed expression microarray on 25 B-cell ALL and 6 Ph-positive B-cell ALL to cluster and identify the transcriptional signature... ALL. Conclusion: In summary, we demonstrate using a gene expression microarray for classifying Ph-like B-cell ALL and highlight VPREB1 as a potential biomarker for this disease.Publication Metadata only A whole genome SNP genotyping by DNA microarray and candidate gene association study for kidney stone disease(2014-05-02) Nanyawan Rungroj; Choochai Nettuwakul; Nirinya Sudtachat; Oranud Praditsap; Nunghathai Sawasdee; Suchai Sritippayawan; Duangporn Chuawattana; Pa thai Yenchitsomanus; Mahidol University; Thailand National Center for Genetic Engineering and BiotechnologyBackground: Kidney stone disease (KSD) is a complex disorder with unknown etiology in majority of the patients. Genetic and environmental factors may cause the disease. In the present study, we used DNA microarray to genotype single nucleotide... polymorphisms (SNP) and performed candidate gene association analysis to determine genetic variations associated with the disease.Methods: A whole genome SNP genotyping by DNA microarray was initially conducted in 101 patients and 105 control subjects. A setItem Metadata only Microarrayed Allergen Molecules Distinguish IgE Sensitisation to Blomia tropicalis and Dermatophagoides pteronyssinus(2026-01-01) Dsouza N.; Phanthong S.; Huang H.J.; Weber M.; Sarzsinszky E.; Zieglmayer P.; Pauli G.; Focke-Tejkl M.; Tulaev M.; Todorovic N.; Keller W.; Schlederer T.; Caraballo L.; Tungtrongchitr A.; Valenta R.; Tantilipikorn P.; Vrtala S.; Dsouza N.; Mahidol University. There is only limited IgE cross-reactivity between Dermatophagoides and Blomia. Objective: To produce a chip containing purified microarrayed Blomia tropicalis (Blo t) and Dermatophagoides pteronyssinus allergens (Der p) capable of identifying patients with a... genuine Blo t or Der p IgE sensitisation, co-sensitisation and/or cross-sensitisation. Methods: Chips containing seven purified Blo t and thirteen Der p allergens were generated by microarray technology and tested for IgE and IgG reactivity in HDMItem Metadata only Mesoporous Silica Nanoparticles-Enhanced Microarray Technology for Highly Sensitive Simultaneous Detection of Multiplex Foodborne Pathogens(2024-01-01) Hormsombut T.; Mekjinda N.; Kalasin S.; Surareungchai W.; Rijiravanich P.; Hormsombut T.; Mahidol University., Shigella spp., Campylobacter spp., Clostridium spp., and Vibrio spp., utilizing antibody-aptamer arrays. To enhance the fluorescence signals on the microarray, the mesoporous silica nanoparticles (MSNs) conjugated with fluorescein, streptavidin, and seven