20 results
Search Results
Now showing 1 - 10 of 20
Publication Metadata only Novel Analytical Platform For Robust Identification of Cell Migration Inhibitors(2020-12-01) Parinyachat Somchai; Kriengkrai Phongkitkarun; Patipark Kueanjinda; Supawan Jamnongsong; Kulthida Vaeteewoottacharn; Vor Luvira; Seiji Okada; Siwanon Jirawatnotai; Somponnat Sampattavanich; Faculty of Medicine, Khon Kaen University; Kumamoto University; Mahidol University; Faculty of Medicine, Siriraj Hospital, Mahidol University© 2020, The Author(s). Wound healing assay is a simple and cost-effective in vitro assay for assessing therapeutic impacts on cell migration. Its key limitation is the possible confoundment by other cellular phenotypes, causing misinterpretation... cell motility difference and extrinsic factors such as cell seeding density can significantly affect final interpretation of therapeutic impacts on cellular phenotypes. Such discrepancy can be rectified by using the actual wound closure time of eachItem Metadata only Jorunnamycin A induces apoptosis in pancreatic ductal adenocarcinoma cells, spheroids, and patient-derived organoids by modulating KRAS-mediated survival pathways(2025-12-01) Khine H.E.E.; Suriya U.; Rungrotmongkol T.; Chamni S.; Lu Y.; Bénard A.; Lan B.; Mukhopadhyay D.; Chang D.; Biankin A.; Schneider-Stock R.; Grützmann R.; Sungthong R.; Pilarsky C.; Chaotham C.; Khine H.E.E.; Mahidol Universityof current therapeutic strategies. This study investigates the therapeutic potential of jorunnamycin A (JA), a bioactive compound derived from the marine sponge Xestospongia, in PDAC. Molecular docking analyses were performed to assess JA’s binding affinity... mutations. Furthermore, western blot analysis was performed to examine the impact the molecular mechanisms underlying JA’s anticancer activity. JA demonstrated potent anticancer activity against PDAC cells, irrespective of their KRAS mutation statusItem Metadata only Impact of the COVID-19 pandemic on direct medical costs of acute coronary syndrome admissions in Yogyakarta, Indonesia(2024-01-01) Dewi P.E.N.; Nagi M.A.; Sunantiwat M.; Thavorncharoensap M.; Nathisuwan S.; Riewpaiboon A.; Youngkong S.; Dewi P.E.N.; Mahidol UniversityThe number of admissions, therapeutic management, and clinical results of acute coronary syndrome (ACS) have been extensively reported. However, the economic impact of ACS therapy has been ignored. This study examined the effect of the COVID-19... provider’s perspective were estimated using the charges. All costs were reported in Indonesian Rupiah (IDR) at a 2021 value. Multiple linear regression analysis was used to evaluate the impact of the pandemic on total direct medical costs. The total directItem Metadata only Therapeutic potential of Thai herbal formula for cognitive impairment: A metabolomics approach for Comprehensive Insights(2024-04-15) Akarasereenont P.; Pattanapholkornsakul S.; Limsuvan S.; Mamaethong D.; Booranasubkajorn S.; Pakaprot N.; Tripatara P.; Pilakasiri K.; Akarasereenont P.; Mahidol Universitymetabolomic changes after YHF20 administration in a rat model of permanent bilateral common carotid artery occlusion (2-VO) induced chronic cerebral ischemia hypoperfusion, and to explore its impact on spatial learning and memory. Albino Wistar rats wereItem Metadata only Impact of resistant starch type 3 on fecal microbiota and stool frequency in Thai adults with chronic constipation randomized clinical trial(2024-12-01) Luk-In S.; Leepiyasakulchai C.; Saelee C.; Keeratichamroen A.; Srisangwan N.; Ponprachanuvut P.; Chammari K.; Chatsuwan T.; Wannigama D.L.; Shein A.M.S.; Kueakulpattana N.; Srisakul S.; Sranacharoenpong K.; Luk-In S.; Mahidol UniversityConstipation poses a significant health concern affecting individuals of varying ages and geographic locations worldwide. While the impacts of numerous probiotics on constipation are well-characterized, there has been limited assessment... reporting these as healthy stools. Our findings provide important insights into the therapeutic advantages of RS-3 for constipation, and propose RS-3 as a feasible alternative strategy for management of constipation.Item Metadata only Impact of AbaI mutation on virulence, biofilm development, and antibiotic susceptibility in Acinetobacter baumannii(2024-12-01) Pumirat P.; Santajit S.; Tunyong W.; Kong-Ngoen T.; Tandhavanant S.; Lohitthai S.; Rungruengkitkun A.; Chantratita N.; Ampawong S.; Reamtong O.; Indrawattana N.; Pumirat P.; Mahidol University-based allele replacement method to investigate the impact of abaI on A. baumannii. Proteomic analysis revealed significant alterations in protein expression between the wild type (WT) and ΔabaI mutant strains, particularly in proteins associated... in modulating various cellular processes and highlight its significance in A. baumannii physiology, pathogenesis, and antibiotic resistance. Targeting the abaI QS system may offer novel therapeutic strategies for this clinically significant pathogen.Publication Metadata only Relationship between CYP2D6 genotype, activity score and phenotype in a pediatric Thai population treated with risperidone(2021-12-01) Yaowaluck Hongkaew; Andrea Gaedigk; Bob Wilffert; Nattawat Ngamsamut; Wiranpat Kittitharaphan; Penkhae Limsila; Chonlaphat Sukasem; Ramathibodi Hospital; Bumrungrad International Hospital; Faculty of Medicine Ramathibodi Hospital, Mahidol University; Thailand Ministry of Public Health; Rijksuniversiteit Groningen; University of Missouri-Kansas City; Universitair Medisch Centrum Groningen; Division of Clinical Pharmacology, Toxicology and Therapeutic Innovationimpacts risperidone metabolism, we demonstrated that revised value for the CYP2D6*10 was superior for genotype to phenotype translation. However, at least for risperidone, subjects with an activity score of 1 presented as phenotypic normalItem Metadata only Antioxidative and anticancer effects of Tacca chantrieri extract enhancing cisplatin sensitivity in cholangiocarcinoma cells(2025-01-01) Armartmuntree N.; Kittirat Y.; Promraksa B.; Loilome W.; Dokduang H.; Techasen A.; Sansomchai P.; Thanee M.; O’Connor T.; Kongthitilerd P.; Padthaisong S.; Armartmuntree N.; Mahidol Universitywith minimal adverse side effects. This study explored the potential of Tacca chantrieri (TC), a plant rich in bioactive compounds, as a therapeutic agent for CCA. TC, a traditional remedy in Southeast Asia, exhibits anti-inflammatory and cytotoxic properties... extracts on CCA cell lines (KKU-213A and KKU-213C) were evaluated using MTT assays and flow cytometry. Protein levels of Bax and Bcl-2 were determined through western blot analysis. Additionally, the study investigated whether the combined impact of TCItem Unknown Analysis of whiB7 in Mycobacterium tuberculosis reveals novel AT-hook deletion mutations(2023-12-01) Davies-Bolorunduro O.F.; Jaemsai B.; Ruangchai W.; Phumiphanjarphak W.; Aiewsakun P.; Palittapongarnpim P.; Mahidol Universitysequences of whiB7 of over 40,000 diverse Mtb isolates. Our results unveil the dominant c.191delG (Gly64delG) mutation, present in all members of the lineage L1.2.2 and its impact on WhiB7's conserved GVWGG-motif, causing conformational changes and deletion... for the development of novel therapeutic interventions.Item Unknown VP28 interacts with PmRab7 irrespective of its nucleotide state(2024-12-01) Sudsat P.; Srisala J.; Pakotiprapha D.; Tapaneeyakorn S.; Sritunyalucksana K.; Thitamadee S.; Charoensutthivarakul S.; Itsathitphaisarn O.; Sudsat P.; Mahidol UniversityIn shrimp aquaculture, white spot syndrome virus (WSSV) infections severely impact production. Previous research highlighted the crucial role of the Penaeus monodon Rab7 (PmRab7) protein in WSSV entry, specifically its interaction with the viral... higher binding affinity than in the absence (WT, 1920 nM) indicating that the addition of excess nucleotide for PmRab7-WT enhanced the affinity for VP28. Together, these findings support the potential of PmRab7-WT as a promising therapeutic candidate