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Publication Open Access Phenotypic characterizations and genetic study of progressive rod-cone degeneration in poodles in Thailand(2014) Metita Sussadee; Janjira Phavaphutanon; Chaiyan Kasorndorkbua; Areerat Kongcharoen; Preenun Jitasombuti; Sudtisa Laopiem; Aree Thayananuphat; Mahidol University. Faculty of Veterinary Science. Veterinary Teaching HospitalProgressive rod-cone degeneration (PRCD) is one of several groups of inherited progressive retinal atrophy in dogs. This study aims to describe the characterization of phenotypic appearance and demonstrate the responsible gene mutation, including... analysis of the allele frequency of PRCD in Poodle dogs in Thailand. All 10 Poodles with clinical signs of PRCD were identified by a history of progressive vision loss, an abnormal obstacle test, and fundic appearance. Genetic testing of PRCD gene mutationPublication Open Access Experimental infection with a Thai reassortant swine influenza virus of pandemic H1N1 origin induced disease.(2013) Nataya Charoenvisal; Juthatip Keawcharoen; Donruethai Sreta; Siriporn Tantawet; Suphattra Jittimanee; Jirapat Arunorat; Alongkorn Amonsin; Roongroje Thanawongnuwech; Mahidol University. Faculty of Veterinary Science. Department of Clinical Science and Public Health.Background Following the emergence of the pandemic H1N1 influenza A virus in 2009 in humans, this novel virus spread into the swine population. Pigs represent a potential host for this virus and can serve as a mixing vessel for genetic mutations... animals in the rH1N1-infected cohort demonstrated more severe clinical signs, had higher numbers of pigs shedding the virus, were noted to have increased histopathological severity of lung lesions and increased viral antigen in lung tissue, the findingsPublication Open Access ผลของสารสกัดช้าพลู (Piper sarmentosum) ที่มีต่อความเสียหายของโครโมโซมของเซลล์กระดูกของหนูขาว ทดสอบโดยวิธีดูไมโครนิวเคลียส(2004-06) ปรานอม ภูชฎาภิรมย์; ปัญญา เต็มเจริญ; สุรพล คงทิม; ลักขณา หิมะคุณ; ยุวดี วงษ์กระจ่าง; เพ็ญโฉม พึ่งวิชา; รุ่งระวี เต็มศิริฤกษ์กุล; มหาวิทยาลัยมหิดล. คณะวิทยาศาสตร์. ภาควิชาพยาธิชีววิทยา; มหาวิทยาลัยมหิดล. คณะเภสัชศาสตร์. ภาควิชาสรีรวิทยา; มหาวิทยาลัยมหิดล. คณะเภสัชศาสตร์. ภาควิชาเภสัชพฤกษศาสตร์of the water extract of Chaplu, respectively. Animals were killed at 30 hours after treatment. In addition, for the dose of 10 g/kg body weight the animals were also killed at 24, 48 and 72 hours after treatment. Bone marrow cells were collected and 2000... polychromatic erythrocytes (PCEs) per animals were analyzed for the presence of micronuclei. Four hundred erythrocytes were also scored for the PCEs: normochromatic erythrocytes (NCEs) ratio. The water extract of Chaplu at the dose up to 10 g/kg body weightPublication Open Access Subtilisin-like proprotein convertases (SPCs); host enzymes controlled viral protein processing and maturation.(2014) Kridsada Chaichoun; Mahidol University. Faculty of Veterinary Science. Department of Pre-Clinical and Applied Animal ScienceThe cellular subtilisin-like proprotein convertases (SPCs) are responsible for virion maturation process which occurs in secretory vesicles, primes virion maturation and viral infectivity. Eight SPCs, SPC1 (furin/PACE), SPC2 (PC2), SPC3 (PC1/PC3), SPC4 (PACE4), SPC5 (PC4), SPC6 (PC5/PC6A) and SPC7 (LPC/PC7/PC8) and PCSK9, were identified. The consensus substrate sequence is -RX(K/R)R▼X- (X can be any amino acid, ▼represents the cleavage site). The conformational change of viral proteins can be triggered by a low pH in the endosomes, as in the case of influenza virus, or by the interaction with a secondary receptor protein at the cell surface, as the case of HIV. In flaviviruses, the functional roles of charged residues locate to the SPC consensus sequence in cleavage site of prM protein and provide cleavability affect to virus replication. Changes in the prM-cleavage level were associated with altered proportions of extracellular virions and subviral particles. The hemagglutinin (HA) protein is a critical determinant of the pathogenicity of avian influenza viruses, with a clear link between HA cleavability and virulence. The highly pathogenic avian influenza virus, in which contain high numbers of basic amino acid sequence at the HA cleavage site, can be converted to low numbers of basic amino acid sequence of a typical avirulent virus. The processing by SPCs is an important control mechanism for the biological activity of viral surface proteins. The molecular mechanisms underlying the recognition of SPCs by viral glycoproteins were described, including recent findings demonstrating differential SPC-recognition of viral and cellular substrates. Proteolytic activation of envelope glycoproteins is necessary for entry of viruses into the host cell and, hence, for their ability to undergo multiple replication cycles. Proteolytic cleavage is the first step in the activation of virus fusion proteins and is followed by a conformational change resulting in the exposure of the fusion domain. The conformational change can be triggered by a low pH in the endosomes, or by the interaction with a secondary receptor protein at the cell surface.Publication Open Access Extended loop region of Hcp1 is critical for the assembly and function of type VI secretion system in Burkholderia pseudomallei.(2015-02-04) Lim, Yan Ting; Jobichen, Chacko; Wong, Jocelyn; Direk Limmathurotsakul; ดิเรก ลิ้มมธุรสกุล; Li, Shaowei; Chen, Yahua; Raida, Manfred; Nalini Srinivasan; MacAry, Paul Anthony; Sivaraman, J.; Gan, Yunn-Hwen; Sivaraman, J.; Gan, Yunn-Hwen; Mahidol University. Faculty of Tropical Medicine. Department of Tropical Hygiene and Mahidol-Oxford Tropical Medicine Research Unit.point between adjacent hexameric rings. When two residues within the loop were mutated, the mutant proteins were unable to stack as dodecamers, suggesting defective tube assembly. Moreover, infection with a bacterial mutant containing in situPublication Open Access Preliminary report: homology modeling of human ryanodine receptor-1(2012) Waraphan Toniti; Pranom Puchadapirom; Aekkapot Chamkasem; Mahidol University. Faculty of Veterinary Science. Department of Pre-clinic and Applied Animal Sciences; Mahidol University. Faculty of Science. Department of Pathobiology; Mahidol University. Faculty of Veterinary ScienceExcitation-contraction (E-C) coupling is the series of events in which an electrical stimulus is converted into a mechanical contraction. Ryanodine receptors (RyRs), the Ca2+ release channels, located at the sarcoplasmic reticulum membrane and played role in E-C coupling. In this study, human RyR1sequence was studied by sequence of P21817. The in silico RyR1 models were generated using homology modeling. RyR1 is the largest known ion channels and composes of 15 important subdomains; cytoplasmic assembly and transmembrane assembly. This study focused on the larger cytoplasmic assembly that is composed of 10 subdomains. The results show that the shapes and the pocket sites of each domain of RyR1 are different. Each domain has its own pocket sites which facilitateinteraction between RyR1 and modulators. Future studies will certainly resolve additional structural differences among species of interest and may apply as model of calcium release channel-modulator interaction.Publication Open Access The importance of epigenetics in embryonic development and reproductive biotechnology(2012) Tuempong Wongtawan; Mahidol University. Faculty of Veterinary Science. Department of Pre-clinic and Applied Animal ScienceIt is well established that epigenetics and chromatin modifications are primary factors that can govern gene activity and nuclear architecture. They are also proven to be essential for normal embryonic development and cell differentiation. One important step during mouse development is the establishment of epigenetic reprogramming which is believed to be vital for normal growth and development, however; the mechanism is still poorly understood. Creating embryo using reproductive biotechnology such as IVM/IVF, ICSI and nuclear transfer may cause abnormal epigenetic pattern. This review summarise the role and the importance of epigenetic in embryonic development as well as reproductive biotechnology from research literature mainly in mouse used as a model for mammalian development.Publication Open Access Human monoclonal ScFv that bind to different functional domains of M2 and inhibit H5N1 influenza virus replication(2013) Tippawan Pissawong; Santi Maneewatch; Kanyarat Thueng-in; Potjanee Srimanote; Fonthip Dong-din-on; Jeeraphong Thanongsaksrikul; Thaweesak Songserm; Pongsri Tongtawe; Kunan Bangphoomi; Wanpen Chaicumpa; Mahidol University. Faculty of Medicine Siriraj Hospital. Laboratory for Research and Technology Development. While the molecular mechanisms of the HuScFv await experimental validation, the small human antibody fragments have high potential for developing further as a safe, novel and mutation tolerable anti-influenza agent especially against drug resistantPublication Open Access A single-tube allele specific-polymerase chain reaction to detect T315I resistant mutation in chronic myeloid leukemia patients(2011) Wanwisa Wongboonma; Wanna Thongnoppakhun; Chirayu U Auewarakul; Mahidol University. Faculty of Medicine Siriraj Hospital. Department of MedicineBackground: BCR-ABL kinase domain (KD) mutation is the major mechanism contributing to suboptimal response to tyrosine kinase inhibitors (TKI) in BCR-ABL-positive chronic myeloid leukemia (CML) patients. T315I mutation, as one of the most frequent... KD mutations, has been shown to be strongly associated with TKI resistance and subsequent therapeutic failure. A simple and sensitive method is thus required to detect T315I mutation at the earliest stage. Methods: A single-tube allele specificPublication Open Access Genetic variations in regions of bovine and bovine-like enteroviral 5’UTR from cattle, Indian bison and goat feces(2016) Nathamon Kosoltanapiwat; Marnoch Yindee; Chavez, Irwin Fernandez; Pornsawan Leaungwutiwong; Poom Adisakwattana; Pratap Singhasivanon; Charin Thawornkuno; Narin Thippornchai; Amporn Rungruengkitkun; Juthamas Soontorn; Sasipan Pearsiriwuttipong; Mahidol University. Faculty of Tropical Medicine. Department of Microbiology and ImmunologyBackground: Bovine enteroviruses (BEV) are members of the genus Enterovirus in the family Picornaviridae. They are predominantly isolated from cattle feces, but also are detected in feces of other animals, including goats and deer. These viruses... are found in apparently healthy animals, as well as in animals with clinical signs and several studies reported recently suggest a potential role of BEV in causing disease in animals. In this study, we surveyed the presence of BEV in domestic and wild