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Publication Open Access Birth of kittens after the transfer of frozen-thawed embryos produced by intracytoplasmic sperm injection with spermatozoa collected from cryopreserved testicular tissue.(2012) T Tharasanit; S Buarpung; S Manee-In; C Thongkittidilok; N Tiptanavattana; P Comizzoli; M Techakumphu; Mahidol University. Faculty of Veterinary Science. Department of Clinical Science and Public HealthThe aim of this study is to produce live kittens from oocytes fertilized by intracytoplasmic sperm injection (ICSI) with frozen/thawed testicular spermatozoa. Spermatozoa were collected from thawed testicular tissue and subsequently injected into in vitro matured cat oocytes. At 24 h post-ICSI, presumptive zygotes/cleaved embryos were treated with 10 μm forskolin for 24 h to reduce intracellular lipid content of embryos (delipidation). At 48 h after oocyte injection, cleaved embryos (2- to 8-cell stage) were frozen in 10% (v/v) ethylene glycol-based medium by a slow controlled rate method and stored in liquid nitrogen. To evaluate in vitro and in vivo developmental competence, frozen embryos were thawed and then cultured for 6 days (n = 155) or cultured for 2 h before transferred (n = 209) to hormonal (equine chorionic gonadotropin/hCG)-treated cat recipients. Cleavage frequency at day 2 after ICSI with frozen/thawed testicular spermatozoa was ~30%. The percentages of frozen/thawed embryos that developed to morula and blastocyst stage (on day 3 and day 6 of in vitro culture, respectively) were significantly lower than that of fresh ICSI embryos (22.6 vs 45.2% and 21.3 vs 38.7%, respectively; p < 0.05). However, no difference was found in the number of blastomeres between frozen/thawed (242.5 ± 43.1) and fresh (320.2 ± 28.1) blastocysts. Three of seven cat recipients were pregnant and one pregnant cat delivered two healthy kittens. This is the first report of the birth of kittens after the transfer of frozen-thawed embryos produced by ICSI with frozen/thawed testicular sperm.Publication Open Access Effects of vitrification on nuclear maturation, ultrastructural changes and gene expression of canine oocytes(2010) Bongkoch Turathum; Kulnasan Saikhun; Parisatcha Sangsuwan; Yindee Kitiyanant; Mahidol University. Institute of Molecular Biosciences; Mahidol University. Faculty of Science. Department of AnatomyBackground Cryopreservation of oocytes, which is an interesting procedure to conserve female gametes, is an essential part of reproductive biotechnology. The objective of the present study was to investigate the effects of vitrification on nuclearPublication Open Access Multidimensional indicators of healthy women in reproductive age(2008) Boonthum, A; Angsana Boonthum; Theerawanviwat, D; อังสนา บุญธรรมThe purposes of this study are to describe indicators of healthy women at reproductive age in each dimension affecting their healthy condition and to examine the internal structure of various dimensions of a reproductive age construct. A survey... group of six dimensions: physical health, mental health, social health, health-promoting behavior, health risk behavior and reproductive health. Structural equation modeling was employed to examine the theoretical models of the Healthy WomenPublication Open Access The importance of epigenetics in embryonic development and reproductive biotechnology(2012) Tuempong Wongtawan; Mahidol University. Faculty of Veterinary Science. Department of Pre-clinic and Applied Animal Scienceimportant step during mouse development is the establishment of epigenetic reprogramming which is believed to be vital for normal growth and development, however; the mechanism is still poorly understood. Creating embryo using reproductive biotechnology... such as IVM/IVF, ICSI and nuclear transfer may cause abnormal epigenetic pattern. This review summarise the role and the importance of epigenetic in embryonic development as well as reproductive biotechnology from research literature mainly in mouse used as aPublication Open Access Application of smart phone in "Better Border Healthcare Program": a module for mother and child care(2010-11) Jaranit Kaewkungwal; จรณิต แก้วกังวาล; Pratap Singhasivanon; ประตาป สิงหศิวานนท์; Amnat Khamsiriwatchara; อำนาจ คำศิริวัชรา; Surasak Sawang; Pongthep Meankaew; พงษ์เทพ เมียนแก้ว; Apisit Wechsart; Jaranit Kaewkungwal; Mahidol University. Faculty of Tropical Mediicine. Department of Tropical Hygienecombining web-based and mobile technology was developed to generate ANC/EPI visit schedule dates in which the healthcare personnel can cross-check, identify and update the mother's ANC and child's EPI status at the healthcare facility or at the householdPublication Open Access Customized-language voice survey on mobile devices for text and image data collection among ethnic groups in Thailand: a proof-of-concept study.(2014-03-06) Kasemsak Jandee; เกษมศักดิ์ จันดี; Saranath Lawpoolsri; สารนาถ ล้อพูลศรี; Pimsurang Taechaboonsermsak; พิมพ์สุรางค์ เตชะบุญเสริมศักดิ์; Amnat Khamsiriwatchara; Peerawat Wansatid; Jaranit Kaewkungwal; จรณิต แก้วกังวาล; Jaranit Kaewkungwal; Mahidol University. Faculty of Tropical Medicine. Center of Excellence for Biomedical and Public Health Informatics (BIOPHICS); Mahidol University. Faculty of Tropical Medicine. Department of Tropical Hygiene.; Mahidol University. Faculty of Public Health. Department of Family Health.BACKGROUND: Public health surveys are often conducted using paper-based questionnaires. However, many problems are associated with this method, especially when collecting data among ethnic groups who speak a different language from the survey interviewer. The process can be time-consuming and there is the risk of missing important data due to incomplete surveys. OBJECTIVE: This study was conducted as a proof-of-concept to develop a new electronic tool for data collection, and compare it with standard paper-based questionnaire surveys using the research setting of assessing Knowledge Attitude and Practice (KAP) toward the Expanded Program on Immunization (EPI) among 6 ethnic groups in Chiang Rai Province, Thailand. The two data collection methods were compared on data quality in terms of data completeness and time consumed in collecting the information. In addition, the initiative assessed the participants' satisfaction toward the use of a smartphone customized-language voice-based questionnaire in terms of perceived ease of use and perceived usefulness. METHODS: Following a cross-over design, all study participants were interviewed using two data collection methods after a one-week washout period. Questions in the paper-based questionnaires in Thai language were translated to each ethnic language by the interviewer/translator when interviewing the study participant. The customized-language voice-based questionnaires were programmed to a smartphone tablet in six, selectable dialect languages and used by the trained interviewer when approaching participants. RESULTS: The study revealed positive data quality outcomes when using the smartphone, voice-based questionnaire survey compared with the paper-based questionnaire survey, both in terms of data completeness and time consumed in data collection process. Since the smartphone questionnaire survey was programmed to ask questions in sequence, no data was missing and there were no entry errors. Participants had positive attitudes toward answering the smartphone questionnaire; 69% (48/70) reported they understood the questions easily, 71% (50/70) found it convenient, and 66% (46/70) reported a reduced time in data collection. The smartphone data collection method was acceptable by both the interviewers and by the study participants of different ethnicities. CONCLUSIONS: To our knowledge, this is the first study showing that the application of specific features of mobile devices like smartphone tablets (including dropdown choices, capturing pictures, and voiced questions) can be successfully used for data collection. The mobile device can be effectively used for capturing photos of secondary data and collecting primary data with customized-language and voiced questionnaire survey. Using smartphone questionnaires can minimize or eliminate missing data and reduce the time consumed during the data collection process. Smartphone customized-language, voice-based questionnaires for data collection can be an alternative and better approach than standard translated paper-based questionnaires for public health surveys, especially when collecting data among ethnic and hard-to-reach groups residing in multilanguage-speaking settings.Publication Open Access L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility(2014-06) Manee-In S; Parmornsupornvichit S; Kraiprayoon S; Tharasanit T; Chanapiwat P; Kaeoket K; Mahidol University. Faculty of Veterinary Science. Department of Clinical Sciences and Public Health MedicineCryopreservation of epididymal sperm is an effective technique to preserve genetic materials of domestic cats and wild felids when they unexpectedly die. However, this technique inevitably causes detrimental changes of cryopreserved-thawed spermatozoa, for example, by physical damage and excessive oxidative stress. L-carnitine is an antioxidant that has been used to improve sperm motility in humans and domestic animals. This study aimed to investigate the effects of L-carnitine on cat epididymal sperm quality following cryopreservation and thawing. After routine castration, cauda epididymides were collected from 60 cat testes. The epididymal spermatozoa from 3 cauda epididymides were pooled as 1 replicate. Spermatozoa samples (16 replicates) were examined for spermatozoa quality and then randomly divided into 4 groups: 0 mM L-carnitine (control), 12.5 mM, 25 mM and 50 mM L-carnitine. The sperm aliquots were then equilibrated and conventionally frozen. After thawing, sperm motility, plasma membrane integrity, DNA integrity and acrosome integrity were evaluated. The 25 mM L-carnitine significantly improved sperm motility compared with a control group (p<0.05), although this was not significantly different among other concentrations. In conclusion, supplementation of 25 mM L-carnitine in freezing extender improves cauda epididymal spermatozoa motility. The effects of L-carnitine on the levels of oxidative stress during freezing and thawing remains to be examinedPublication Open Access Implementing comprehensive prevention of mother-to-child transmission and HIV prevention for South African couples: study protocol for a randomized controlled trial(2014) Jones, Deborah; Peltzer, Karl; Weiss, Stephen M; Sifunda, Sibusiso; Dwane, Ntabozuko; Ramlagan, Shandir; Cook, Ryan; Matseke, Gladys; Maduna, Vincent; Spence, Andrew; Mahidol University. ASEAN Institute for Health DevelopmentBackground: In rural South Africa, only two-thirds of HIV-positive pregnant women seeking antenatal care at community health centers took full advantage of ‘prevention of mother-to-child transmission’ (PMTCT) services in 2010. Studies generally support male involvement to promote PMTCT, but the nature and impact of that involvement is unclear and untested. Additionally, stigma, disclosure and intimate partner violence pose significant barriers to PMTCT uptake and retention in care, suggesting that male involvement may be ‘necessary, but not sufficient’ to reduce infant HIV incidence. This study expands on a successful United States President's Emergency Plan for AIDS Relief (PEPFAR)-supported PMTCT couples intervention pilot study conducted in the Mpumalanga province, targeting HIV-positive pregnant women and their partners, the primary objective being to determine whether male partner involvement plus a behavioral intervention will significantly reduce infant HIV incidence. Methods/design: The study follows a cluster randomized controlled design enrolling two cohorts of HIV-positive pregnant women recruited from 12 randomly assigned Community Health Centers (CHC) (six experimental, six control). The two cohorts will consist of women attending without their male partners (n = 720) and women attending with their male partners (n = 720 couples), in order to determine whether the influence of male participation itself, or combined with a behavioral PMTCT intervention, can significantly reduce infant HIV infection ante-, peri- and postnatally. Discussion: It is our intention to significantly increase PMTCT participation from current levels (69%) in the Mpumalanga province to between 90 and 95% through engaging women and couples in a controlled, six session ante- and postnatal risk-reducing and PMTCT promotion intervention addressing barriers to PMTCT (such as stigma, disclosure, intimate partner violence, communication, infant feeding practices and safer conception) that prevent women and men from utilizing treatment opportunities available to them and their infants. Based upon the encouraging preliminary results from our pilot study, successful CHC adoption of the program could have major public health policy implications for containing the epidemic among the most vulnerable populations in rural South Africa: HIV-positive pregnant women and their infants. Trial registration: ClinicalTrials.gov NCT02085356 (registration date: 10 March 2014).Publication Open Access Intestinal capillariasis in the 21st century: clinical presentations and role of endoscopy and imaging(2014) Julajak Limsrivilai; Supot Pongprasobchai; Piyaporn Apisarnthanarak; Sathaporn Manatsathit; Mahidol University. Faculty of Medicine, Siriraj Hospital. Division of Gastroenterology, parasites were discovered in tissue biopsy from endoscopy in 1 from 10 esophagogastroduodenoscopies (EGD), 0 from 7 colonoscopies, 3 from 5 push enteroscopies, and 3 from 5 balloon-assisted enteroscopies (BAE). Endoscopic findings included scallopingPublication Open Access Nymphaea nouchali var. versicolor ‘Bua Phuean’: Seed morphology and germination in vitro(2011-08) Kitti Bodhipadma; Sompoch Noichinda; Phetcharat Wachirabongkoth; Ekawut Pukpoomin; Luepol Punnakanta; Koravisd Nathalang; Mahidol University. Faculty of Environment and Natural Resource Studies; Mahidol University. Faculty of Science. Department of BiologyNymphaea nouchali var. versicolor ‘Bua Phuean’ is one of Thai native species of dayblooming waterlily. Since the expansion of community from metropolitan area and the appreciation on exotic species of Nymphaea, number of this plant in environment is noticeably decreased. One of the efficient methods to conserve and increase the amount of this plant is seed germination. Thus, seed morphology and developmental stages of ‘Bua Phuean’ were investigated before germinating in vitro. The results revealed that ‘Bua Phuean’ seeds could be categorized into 5 stages and each stage also had dissimilar morphology. After the stages 3 to 5 seeds were germinated under aseptic condition, seed in stage 5 showed the highest germination rate at 82.22 %. During seed germination, coleoptile emerged from seed coat before root became visible.