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Publication Open Access ตัวรับฮอร์โมนเอสโตรเจนและโปรเจสเตอโรนในเนื้อเยื่อเต้านมสุนัข(2552) สุกัญญา มณีอินทร์; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์, the studies of sex hormone manipulation might be important for hormone-dependent mammary tumors.Publication Open Access Thermoregulatory correlates of nausea in rats and musk shrews(2014) Sukonthar Ngampramuan; Matteo Cerri; Flavia Del Vecchio; Corrigan, Joshua J; Amornrat Kamphee; Dragic, Alexander S; Rudd, John A; Romanovsky, Andrej A; Eugene Nalivaiko; Mahidol University. Institute of Molecular BiosciencesNausea is a prominent symptom and major cause of complaint for patients receiving anticancer chemo- or radiation therapy. The arsenal of anti-nausea drugs is limited, and their efficacy is questionable. Currently, the development of new compounds...-HT3 receptor antagonist, which is used to treat nausea in patients in chemo- or radiation therapy, attenuated hypothermia by ~30%. In shrews, motion sickness was induced by a cyclical backand-forth motion (4 cm, 1 Hz, 15 min) and confirmedPublication Open Access Hybrid approach for left-sided colonic carcinoma obstruction; a case report(2011) Atthaphorn Trakarnsanga; Thawatchai Akaraviputh; Asada Methasate; Vitoon Chinswangwatanakul; Mahidol University. Faculty of Medicine Siriraj Hospital. Minimally Invasive Surgery unitTraditionally, there are several approaches to manage left-sided colonic carcinoma obstruction, such as tumor resection with primary anastomosis, tumor resection with end-colostomy and loop-colostomy. Recently, colonic stent insertion was introduced as a bridge prior to definite surgery. We demonstrated a hybrid approach for obstructed sigmoid carcinoma using colonic stent, followed by single incision laparoscopic colectomy (SILC). A 58 year-old man presented with complete left-sided colonic obstruction. He underwent emergency colonoscopy with metallic stent placement. One week later, he was performed SILC. He recovered well after the operation without any postoperative complications. The pathological result showed adequacy of oncologic resection. This hybrid approach of colonic stent insertion and SILC can be safely performed.Publication Open Access In vitro antimicrobial effects of mangosteen extract on peri-implantitis microflora in craniofacial implants.(2013-09) Shrestha, Binit; Sroisiri Thaweboon; สร้อยศิริ ทวีบูรณ์; Suwan Choonharuangdej; สุวรรณ ชุณหเรืองเดช; Boonyanit Thaweboon; บุญนิตย์ ทวีบูรณ์; Theerathavaj Srithavaj; ธีรธวัช ศรีธวัช; Sita Thaworanunta; สิตา ถาวรนันท์; Boonyanit Thaweboon; บุญนิตย์ ทวีบูรณ์; Mahidol University. Faculty of Dentistry. Maxillofacial Prosthetic Service; Mahidol University. Faculty of Dentistry. Department of Oral MicrobiologyIntroduction: Craniofacial implants has enabled restoration of congenital and acquired facial defects. However, peri-implantitis of the skin-abutment interface is a common drawback following implant placement. Objective: To determine the antimicrobial effects of mangosteen pericarp extract on peri-implantitis microflora found around craniofacial implants. Material and Methods: The mangosteen pericarp extract was tested against peri-implantitis microflora reference strains of Staphylococcus aureus ATCC6538, Candida albicans ATCC10231 and clinical strains of Staphylococcus aureus and Candida parapsilosis by disk diffusion test. Minimum inhibitory concentrations (MIC) and minimum cidal concentrations (MCC) were determined using modified agar dilution millipore method. The extract was further combined with a 50:50 mixture of polyethylene glycol and propylene glycol to form a paste and tested for antimicrobial effects. Results: Mangosteen extract showed inhibitory effects with reference strain of S. aureus at MIC and MCC at 1.25 mg/mL and 2.5 mg/mL and clinical strain at 2.5 mg/mL and 5 mg/mL respectively. On the contrary, it showed minimal or no reactivity against C. albicans and C. parapsilosis. The combination of the extract with polyethylene glycol and propylene glycol also showed a dose dependent inhibitory effect on S. aureus. Conclusion: Mangosteen extract had potential antimicrobial effects against S. aureus, which can be further studied and developed, to be used in the treatment of microorganism induced infection of skin-abutment interface of craniofacial implants.Publication Open Access Characterization of physiologic occlusion.(2014-09) Nattaya Asawaworarit; ณัฏยา อัศววรฤทธิ์; Somsak Mitrirattanakul; สมศักดิ์ ไมตรีรัตนะกุล; Somsak Mitrirattanakul; สมศักดิ์ ไมตรีรัตนะกุล; Mahidol University. Faculty of Dentistry. Department of Masticatory ScienceObjectives: To characterize and describe the occlusal relationship in Thai subjects with physiologic occlusion. Materials and methods: One hundred and twenty-four subjects (49 males and 75 females) fulfilled the criteria of physiologic occlusion by Mohl (1988) were examined for the static and dynamic occlusal relationship. Total number of tooth, horizontal and vertical overlap, Angle’s classification were examined for static occlusion. For dynamic occlusion, maximum mouth opening including lateral and protrusive excursions were examined. In addition, the subjects’ occlusal schemes were classified according to the definition from Glossary of Prosthodontic Terms (8th edition) as well as the presence of occlusal interferences during mandibular excursions. Discrepancy between retruded contact positon (RCP) and intercuspal position (ICP) was also performed. Results: The majority of the populations with physiologic occlusion had total number of existing teeth of 28 and Angle’s classification class I. The mean vertical overlap and horizontal overlap were 1.88±1.25 and 2.33±1.31 mm respectively. The average maximum mouth opening, left and right excursive / protrusive movement was 46.52±4.57, 8.77±1.3, 8.55±1.23, and 6.28±1.55 mm respectively. The most common occlusal scheme was group function. Occlusal interference was presented in 20.2% of the population. The most common occlusal interference was protrusive interference (12.1%). About 80% possessed no RCP-MIP discrepancy with the average of 0.15±.0.3 mm. Conclusions: The common occlusal characteristics of physiologic occlusion among Thais are group function occlusal scheme, Angle’s classification I, absence of occlusal interference, approximate 45 mm of maximum mouth opening and absence of RCP-ICP discrepancy.Publication Open Access A systematic review and meta-analysis of randomised controlled trials of delayed primary wound closure in contaminated abdominal wounds(2014) Boonying Siribumrungwong; Pinit Noorit; Chumpon Wilasrusmee; Ammarin Thakkinstian; Mahidol University. Faculty of Medicine, Ramathibodi Hospital. Section for Clinical Epidemiology and BiostatisticsA systematic review and meta-analysis was conducted to compare surgical site infection (SSI) between delayed primary (DPC) and primary wound closure (PC) in complicated appendicitis and other contaminated abdominal wounds. Medline and Scopus were searched from their beginning to November 2013 to identify randomised controlled trials (RCTs) comparing SSI and length of stay between DPC and PC. Studies’ selection, data extraction, and risk of bias assessment were done by two independent authors. The risk ratio and unstandardised mean difference were pooled for SSI and length of stay, respectively. Among 8 eligible studies, 5 studies were done in complicated appendicitis, 2 with mixed complicated appendicitis and other types of abdominal operation and 1 with ileostomy closure. Most studies (75%) had high risk of bias in sequence generation and allocation concealment. Among 6 RCTs of complicated appendicitis underwent open appendectomy, the SSI between PC and DPC were not significantly different with a risk ratio of 0.89 (95% CI: 0.46, 1.73). DPC had a significantly 1.6 days (95% CI: 1.41, 1.79) longer length of stay than PC. Our evidence suggested there might be no advantage of DPC over PC in reducing SSI in complicated appendicitis. However, this was based on a small number of studies with low quality. A large scale RCT is further required.Publication Open Access Differential effects of binge methamphetamine injections on the mRNA expression of histone deacetylases (HDACs) in the rat striatum(2014) Omonijo Oluwaseyi; Pawaris Wongprayoon; Ladenheim Bruce; McCoy, Michael T; Piyarat Govitrapong; Jayanthi Subramaniam; Cadeta, Jean Lud; Mahidol University. Institute of Molecular BiosciencesMethamphetamine use disorder is characterized by recurrent binge episodes. Humans addicted to methamphetamine experience various degrees of cognitive deficits and show evidence of neurodegenerative processes in the brain. Binge injections of METH to rodents also cause significant toxic changes in the brain. In addition, this pattern of METH injections can alter gene expression in the dorsal striatum. Gene expression is regulated, in part, by histone deacetylation. We thus tested the possibility that METH toxic doses might cause changes in the mRNA levels of histone deacetylases (HDACs). We found that METH did produce significant decreases in the mRNA expression of HDAC8, which is a class I HDAC. METH also decreased expression of HDAC6, HDAC9, and HDAC10 that are class II HDACs. The expression of the class IV HDAC, HDAC11, was also suppressed by METH. The expression of Sirt2, Sirt5, and Sirt6 that are members of class III HDACs was also downregulated by METH injections. Our findings implicate changes in HDAC expression may be an early indicator of impending METH-induced neurotoxicity in the striatum. This idea is consistent with the accumulated evidence that some HDACs are involved in neurodegenerative processes in the brain.Publication Open Access ปัจจัยทำนายการฟื้นตัวด้านสรีระของผู้ป่วยหลังผ่าตัดเปลี่ยนข้อเข่าเทียม(2559) ธิราวรรณ เชื้อตาเล็ง; Thirawan Chuetalent; มหาวิทยาลัยมหิดล. คณะพยาบาลศาสตร์. ภาควิชาการพยาบาลศัลยศาสตร์วัตถุประสงค์: เพื่อศึกษาอำนาจการทำนายของดัชนีมวลกาย ความรุนแรงของอาการ และการสนับสนุนทางสังคมกับการฟื้นตัวด้านสรีระของผู้ป่วยหลังผ่าตัดเปลี่ยนข้อเข่าเทียม รูปแบบการวิจัย: การศึกษาความสัมพันธ์เชิงทำนาย (correlation predictive study) วิธีดำเนินการวิจัย: กลุ่มตัวอย่างเป็นผู้ป่วยข้อเข่าเสื่อมที่เข้ารับการรักษาด้วยการผ่าตัดเปลี่ยน ข้อเข่าเทียมเป็นครั้งแรก คัดเลือกกลุ่มตัวอย่างตามเกณฑ์ที่กำหนดจำนวน 95 คน รวบรวมข้อมูลก่อนผ่าตัดโดยใช้แบบสอบถามข้อมูลส่วนบุคคล แบบสอบถามความรุนแรงของอาการ แบบสอบถามการสนับสนุนทางสังคม และประเมินการฟื้นตัวด้านสรีระหลังผ่าตัดด้วยวิธีทดสอบการทรงตัว และการเดินก่อนจำหน่ายออกจากโรงพยาบาล วิเคราะห์ข้อมูลด้วยสถิติการวิเคราะห์ถดถอยเชิงพหุ ผลการวิจัย: กลุ่มตัวอย่างมีการฟื้นตัวด้านสรีระซึ่งทดสอบด้วยการทรงตัว และการเดินระยะเวลาที่ใช้มีค่าเฉลี่ย 94.56 วินาที (SD = 25.69) ดัชนีมวลกาย ความรุนแรงของอาการ และการสนับสนุนทางสังคมสามารถร่วมกันทำนายการฟื้นตัวด้านสรีระ ร้อยละ 24 (R2 = .24, F = 9.56, p < .05) โดยดัชนีมวลกายเป็นตัวแปรเพียงตัวเดียวที่สามารถทำนายการฟื้นตัวด้านสรีระของผู้ป่วยหลังผ่าตัดเปลี่ยนข้อเข่าเทียมได้อย่างมีนัยสำคัญทางสถิติ (β = .484, p < .05). สรุปและข้อเสนอแนะ: จากผลการวิจัย พบว่า ดัชนีมวลกายเป็นปัจจัยที่สามารถทำนายการฟื้นตัวด้านสรีระของผู้ป่วยหลังผ่าตัดเปลี่ยนข้อเข่าเทียม ดังนั้นการควบคุมดัชนีมวลกายก่อนผ่าตัดจึงมีความสำคัญเพื่อการฟื้นตัวสำหรับผู้ป่วยข้อเข่าเสื่อมที่จะได้รับการผ่าตัดเปลี่ยนข้อเข่าเทียมPublication Open Access A member of the CPW-WPC protein family is expressed in and localized to the surface of developing ookinetes.(2013-04-15) Niwat Kangwanrangsan; Tachibana, Mayum; Rachaneeporn Jenwithisuk; รัชนีพร เจนวิถีสุข; Tsuboi, Takafumi; Suda Riengrojpitak; Torii, Motom; Ishino, Tomoko; Ishino, Tomoko; Mahidol University. Faculty of Tropical Medicine. Mahidol Vivax Research Unit; Mahidol University. Faculty of Science. Department of PathobiologyBACKGROUND: Despite the development of malaria control programs, billions of people are still at risk for this infectious disease. Recently, the idea of the transmission-blocking vaccine, which works by interrupting the infection of mosquitoes by parasites, has gained attention as a promising strategy for malaria control and eradication. To date, a limited number of surface proteins have been identified in mosquito-stage parasites and investigated as potential targets for transmission-blocking vaccines. Therefore, for the development of effective transmission-blocking strategies in epidemic areas, it is necessary to identify novel zygote/ookinete surface proteins as candidate antigens. METHODS: Since the expression of many zygote/ookinete proteins is regulated post-transcriptionally, proteins that are regulated by well-known translational mediators were focused. Through in silico screening, CPW-WPC family proteins were selected as potential zygote/ookinete surface proteins. All experiments were performed in the rodent malaria parasite, Plasmodium yoelii XNL. mRNA and protein expression profiles were examined by RT-PCR and western blotting, respectively, over the course of the life cycle of the malaria parasite. Protein function was also investigated by the generation of gene-disrupted transgenic parasites. RESULTS: The CPW-WPC protein family, named after the unique WxC repeat domains, is highly conserved among Plasmodium species. It is revealed that CPW-WPC mRNA transcripts are transcribed in gametocytes, while CPW-WPC proteins are expressed in zygote/ookinete-stage parasites. Localization analysis reveals that one of the CPW-WPC family members, designated as PyCPW-WPC-1, is a novel zygote/ookinete stage-specific surface protein. Targeted disruption of the pycpw-wpc-1 gene caused no obvious defects during ookinete and oocyst formation, suggesting that PyCPW-WPC-1 is not essential for mosquito-stage parasite development. CONCLUSIONS: It is demonstrated that PyCPW-WPC-1 can be classified as a novel, post-transcriptionally regulated zygote/ookinete surface protein. Additional studies are required to determine whether all CPW-WPC family members are also present on the ookinete surface and share similar biological roles during mosquito-stage parasite development. Further investigations of CPW-WPC family proteins may facilitate understanding of parasite biology in the mosquito stage and development of transmission-blocking vaccines.