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Publication Open Access Exploring health practitioners’ acceptability of a prospective semi‑quantitative pfHRP2 device to define severe malaria in the Democratic Republic of Congo(2015) Haan, Freek de; Onyamboko, Marie A.; Fanello, Caterina I.; Woodrow, Charles J.; Yoel Lubell; Boon, Wouter P. C.; Dondorp, Arjen M.; Mahidol University. Faculty of Tropical Medicine. Mahidol-Oxford Tropical Medicine Research UnitBackground: A rapid diagnostic tool is being developed to discern severely ill children with severe malaria from children who are ill with alternative febrile diseases but have coincidental peripheral blood parasitaemia. The device semi...’ acceptability of this prospective diagnostic device in a high malaria transmission setting in the Democratic Republic of Congo. Methods: Data were collected qualitatively by conducting semi-structured interviews with a purposeful sample of healthPublication Open Access Combining electrochemical sensors with miniaturized sample preparation for rapid detection in clinical samples(2014-11-21) Natinan Bunyakul; Antje J. Baeumner; Mahidol University. Faculty of Medical Technology. Department of Clinical ChemistryClinical analyses benefit world-wide from rapid and reliable diagnostics tests. New tests are sought with greatest demand not only for new analytes, but also to reduce costs, complexity and lengthy analysis times of current techniques. Among the myriad of possibilities available today to develop new test systems, amperometric biosensors are prominent players-best represented by the ubiquitous amperometric-based glucose sensors. Electrochemical approaches in general require little and often enough only simple hardware components, are rugged and yet provide low limits of detection. They thus offer many of the desirable attributes for point-of-care/point-of-need tests. This review focuses on investigating the important integration of sample preparation with (primarily electrochemical) biosensors. Sample clean up requirements, miniaturized sample preparation strategies, and their potential integration with sensors will be discussed, focusing on clinical sample analyses.Publication Open Access การคุ้มครองการเรียกเก็บค่าบริการของผู้รับบริการในหน่วยบริการตามพระราชบัญญัติหลักประกันสุขภาพแห่งชาติ พ.ศ.2545 ในกรุงเทพมหานคร(2561) ศิรินภา ใจยะบาล; สุธี อยู่สถาพร; ฉัตรสุมน พฤฒิภิญโญ; ยุวนุช สัตยสมบูรณ์; Sirinapa Jaiyaban; Suthee Usathaporn; Chardsumon Prutipinyo; Youwanuch Sattayasomboon; มหาวิทยาลัยมหิดล. คณะสาธารณสุขศาสตร์. ภาควิชาบริหารงานสาธารณสุขis unable to pay, the doctor will change to use essential drug or medical devices in order not to affect health service units. In case of referral patient, the management will apply the same concept but make additional agreement with their personal health... care unit before using non-essential drugs or medical devices. On the other hand all providers will collect drug or medical device expenses that patients request. When there are complaints, some health service units will refund all complainersPublication Open Access Systematic review and meta-analysis of the effectiveness of continuous glucose monitoring (CGM) on glucose control in diabetes(2013) Nalinee Poolsup; Naeti Suksomboon; Aye Mon Kyaw; Mahidol University. Faculty of Pharmacyand The Cochrane Library up to May 2013 and historical search through the reference lists of relevant articles. There are two types of CGM device: real-time CGM and retrospective CGM and both types of the device were included in the analysis. In T1DM pediatricsPublication Open Access Paper-based Acetylcholinesterase Inhibition Assay Combining a Wet System for Organophosphate and Carbamate Pesticides Detection(2015-02-26) Amara Apilux; Chartchalerm Isarankura-Na-Ayudhya; Virapong Prachayasittikul; Tanawut Tantimongcolwat; Center for Innovation Development and Technology Transfer; Department of Clinical Microbiology and Applied Technologyof organophosphate (OP) and carbamate (CM) pesticides based on the inhibition of acetylcholinesterase (AChE). The paper-based sensor was designed as a foldable device consisting of a cover and detection sheets pre-prepared with indoxyl acetate and AChE... self-contained sample pre-concentration approach greatly enhanced the detection sensitivity. The paper-based device developed here is low-cost, requires minimal reagents and is easy to handle. As such, it would be practically useful for pesticidePublication Open Access Sharps Injuries among Nurses in a Thai Regional Hospital: Prevalence and Risk Factors(2011) Honda M; Masanori Honda; J Chompikul; Jiraporn Chompikul; Rattanapan C; Cheerawit Ratanapan; Wood G; Klungboonkrong S; Mahidol University. ASEAN Institute for Health Developmentand the number of nurses selected proportional to the size of the ward nurse population. 261 self-administered questionnaires were distributed to nurses who used needles, syringes or other sharp medical equipment in their work. Data were analyzed using chiPublication Open Access การประเมินการออกแบบภายในรถพยาบาลฉุกเฉินเพื่อความปลอดภัยในการปฏิบัติงานของเครือข่ายโรงพยาบาลจังหวัดแห่งหนึ่ง(2554) พิพัฒน์ ลักษมีจรัลกุล; สิริกุล พิพิธแสงจันทร์,; ดุสิต สุจิรารัตน์; พิศิษฐ์ วัฒนสมบูรณ์; Pipat Luksamijarulkul; Dusit Sujirarat; Pisit Vatanasomboon; พิพัฒน์ ลักษมีจรัลกุล; มหาวิทยาลัยมหิดล. คณะสาธารณสุขศาสตร์. ภาควิชาจุลชีววิทยา; มหาวิทยาลัยมหิดล. คณะสาธารณสุขศาสตร์. ภาควิชาระบาดวิทยา; มหาวิทยาลัยมหิดล. คณะสาธารณสุขศาสตร์. ภาควิชาวิทยาศาสตร์อนามัยสิ่งแวดล้อม.9% have standard stretcher, and 91% have an appropriate medication. Standard supplies for infection control and injury prevention showed that 25.5% have eye protection equipments, and 53.2% have appropriate seat belts. Data from interviews showed that 80Publication Open Access การศึกษาความถูกต้องและแม่นยำของเครื่องวัดอุณหภูมิทางหูและหน้าผากในผู้ป่วยนอกมีไข้และอาสาสมัครไม่มีไข้(2559) สุนันทา ตั้งปนิธานดี; Sununta Tangpanithandee; วิมลรัตน์ ทองเชื้อ; Wimonrat Thongchuer; อรดี จริตควร; Oradee Charitkuan; มหาวิทยาลัยมหิดล. ศูนย์การแพทย์กาญจนาภิเษก-patients and 120 non-febrile healthy volunteers at Golden Jubilee Medical Center. Each Subject was measured body temperature by oral, forehead, and ear thermometers respectively. Accuracy and precision of the ear and forehead thermometers compared with oral... thermometer as the reference were calculated and graphed using the Bland-Altman method. Bias and precision between two devices were set as below or equal to ± .3; ± .5 of the prior statistical analysis according to experts’ recommendation. Main findingsPublication Open Access Detection of non-amplified mycobacterium tuberculosis genomic DNA using piezoelectric DNA-based biosensors(2010) Thongchai Kaewphinit; Somchai Santiwatanakul; Chamras Promptmas; Kosum ChansiriPiezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a selfassembled monolayer method. The advantage of this study is that a non-amplified genomic bacterial DNA target was used. Instead, the genomic DNA was digested by restriction enzyme to obtain DNA fragments containing the target sequence. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex and other microorganisms. This target DNA preparation, without PCR amplification, will reduce time, costs, and the tedious step of amplification.Publication Open Access Cytotoxicity evaluation of Clinacanthus nutans through dimethylthiazol diphenyltetrazolium bromide and neutral red uptake assays.(2016-01) Suwanna Korsuwannawong; สุวรรณา ก่อสุวรรณวงศ์; La‑Ongthong Vajrabhaya; ละอองทอง วัชราภัย; Suwanna Korsuwannawong; Mahidol University. Faculty of Dentistry. Research OfficeObjectives: The aim of this study was to compare the results of dimethylthiazol diphenyltetrazolium bromide (MTT) and neutral red uptake (NRU) assays of Clinacanthus nutans cytotoxicity. Materials and Methods: Mouse fibroblast (L929) cells were exposed to 0.01%, 0.1%, 0.25%, and 0.5% (W/V) C. nutans in a 96-cluster -well-culture plate for 24 h. The cell viability after exposure to C. nutans was determined by MTT and NRU assays in separate tissue culture plates. The two assays were compared through an intra‑class correlation coefficient (ICC) analysis. Results: No significant differences in cytotoxicity were noted between the two assays ( P > 0.05). The ICC values for agreement between two assays for the negative and positive control groups and C. nutans concentrations of 0.01%, 0.1%, 0.25%, and 0.5% were 0.84, 0.83, 0.77, 0.68, 0.74, and 0.71, respectively. Conclusion: In general, the MTT and NRU assays performed similarly, exhibiting moderate to good correlation for the evaluation of the cytotoxicity of C. nutans .