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Publication Metadata only Detection of bacterial antigen in cerebrospinal fluid in patients with bacterial meningitis: a literature review.(2010-11-01) Somporn Srifuengfung; Kulkunya Chokephaibulkit; Mahidol Universityantigen in cerebrospinal fluid. This article reviewed the methods of bacterial antigen detection for diagnosis of meningitis as well as a microbiology aspect of this life-threatening disease....Rapid detection of bacterial pathogen causing meningitis is very important to guide antimicrobial therapy before the standard culture result is available. Other than gram stain, one of the most useful rapid methods is the detection of bacterialPublication Metadata only Identification of circulating bacterial antigens by in vivo microbial antigen discovery(2011-07-01) Dana E. Nuti; Reva B. Crump; Farida Dwi Handayani; Narisara Chantratita; Sharon J. Peacock; Richard Bowen; Philip L. Felgner; D. Huw Davies; Terry Wu; C. Rick Lyons; Paul J. Brett; Mary N. Burtnick; Thomas R. Kozel; David P. AuCoina; University of Nevada School of Medicine; Mahidol University; University of Cambridge; Colorado State University; University of California, Irvine; University of New Mexico Health Sciences Center; University of South Alabamato identify circulating bacterial antigens. This technique starts with "InMAD serum," which is filtered serum that has been harvested from BALB/c mice infected with a bacterial pathogen. The InMAD serum, which is free of whole bacterial cells, is used... to immunize syngeneic BALB/c mice. The resulting "InMAD immune serum" contains antibodies specific for the soluble microbial antigens present in sera from the infected mice. The InMAD immune serum is then used to probe blots of bacterial lysates or bacterialItem Metadata only The study for the purification of outer membrane complex antigen of Bacteroides fragilis and the production of specific antibody(Mahidol University. Mahidol University Library and Knowledge Center, 1988) Daranee Jamjuree; Anchalee Tansuphasiri; Kanda Vathanophas; Anong Pariyanonda; Pranee Sithisarn; Pipat LuksamijarulkulItem Metadata only Mutagenic and antibacterial tests of newly synthesized sulfa drugs(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Suwanna Nerapattanakul; Thirayudh Glinsukon; Chaivat Toskulkao; Punya Temcharoen, and N-2-pyridylsulfonamide] were also studied for antibacterial activity against six bacterial strains including Candida albican ATCC 10231, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Streptococcus... faecalis and Salmonella typhi by using a standard disk diffusion method. The results showed that all of the ten compounds did not possess any antibacterial activity to all of the six bacterial strains in the testing conditions.Item Metadata only Human Leukocyte Antigen (HLA) System: Genetics and Association with Bacterial and Viral Infections(2022-01-01) Medhasi S.; Chantratita N.; Mahidol UniversityThe human leukocyte antigen (HLA) system is one of the most crucial host factors influencing disease progression in bacterial and viral infections. This review provides the basic concepts of the structure and function of HLA molecules in humans... of bacterial and viral infections would improve the understanding of pathogenesis and identify those at risk from infectious diseases in distinct populations and may improve the individual treatment.Item Metadata only Impact of shipping temperature on cell viability and T cell responses to bacterial antigens(2023-01-01) Rongkard P.; Dunachie S.J.; Kronsteiner B.; Rongkard P.; Mahidol Universityin the majority of samples. Conclusions: Here we demonstrate that shipment of cryopreserved PBMC at -196°C has a benefit on cell viability, recovery and T cell responses to bacterial antigens, although useful information can still be obtained from samples shipped... (dry shipper). After thawing, cell viability and composition were assessed by flow cytometry and antigen specific responses to Burkholderia pseudomallei (BP) were measured using IFN-γ ELISpot. Results: We observed modest lowering of viabilityPublication Metadata only Production of monoclonal antibodies to Vi polysaccharide antigen of Salmonella typhi(1993-01-01) S. Pongsunk; S. Sarasombath; P. Ekpo; P. Tangtherawattana; M. M. Levine; Mahidol Universityin primary bacterial culture and also used to develop an assay to detect Vi antigen in clinical specimens. The result showed that they could not detect the antigen in urine and serum from acute patients even though they could detect as low as 0.02 μg/ml of Vi... antigen added in normal urine. The study has shown that these MAbs are very useful for rapid identification of S. typhi in primary bacterial culture and they can replace polyclonal anti-Vi antibodies which have been used routinely in bacteriologicalItem Metadata only Identification of specific antigen of Pseudomonas pseudomallei and monoclonal antibody production for the diagnosis of melioidosis(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Pacharin Rugdech; Stitaya Sirisinha; Surasakdi WongratanacheewinThe currently used serodiagnostic methods for acute septicemic melioidosis are not yet reliable and satisfactory because of the unavailability of specific antigen. Most reports depend largely on the use of crude unfractionated bacterial extract... for the detection of serum antibodies. In this study, fractionated 19.5-kDa antigen was prepared from unfractionated whole cell antigen of P. pseudomallei and evaluated for its potential usefulness in the diagnosis of acute septicemic melioidosis. The antigenPublication Metadata only CpG DNA, liposome and refined antigen oral cholera vaccine(2003-12-01) Ratree Leelawongtawon; Srinuan Somroop; Urai Chaisri; Pongsri Tongtawe; Manas Chongsa-nguan; Thareerat Kalambaheti; Pramuan Tapchaisri; Sathit Pichyangkul; Yuwaporn Sakolvaree; Hisao Kurazono; Hideo Hayashi; Wanpen Chaicumpa; Mahidol University; Thammasat University; Armed Forces Research Institute of Medical Sciences, Thailand; Okayama University; University of TsukubaWistar rats were divided into nine groups and immunized as follows: the first group received the oral vaccine 1 consisting of the three antigens (LPS, rTcpA and H-CT) associated with a liposome (L) and bacterial CpG-DNA (ODN#1826). The rats of groups 2... and 3 received oral vaccines 2 and 3 consisting of the liposome-associated three antigens with and without non-bacterial CpG-DNA (ODN#1982), respectively. Rats of groups 4 received oral vaccine 4 consisting of the three antigens mixed with the ODN#1826Publication Metadata only Sequential Vaccination With Heterologous Acinetobacter baumannii Strains Induces Broadly Reactive Antibody Responses(2021-07-30) Gathoni Kamuyu; Yat Suen Cheng; Sam Willcocks; Chidchamai Kewcharoenwong; Pattarachai Kiratisin; Peter W. Taylor; Brendan W. Wren; Ganjana Lertmemongkolchai; Richard A. Stabler; Jeremy Brown; Siriraj Hospital; London School of Hygiene & Tropical Medicine; University College London; Khon Kaen University; UCL School of Pharmacy; Chiang Mai Universitywith multiple strains, serum antibodies to protein antigens promoted neutrophil phagocytosis of heterologous A. baumannii strains. In an infection model, mice immunized with multiple strains had lower bacterial counts in the spleen and liver following challenge... conserved protein antigens rather than the capsular polysaccharides. We have immunized mice with single or multiple A. baumannii strains to induce antibody responses to protein antigens, and then assessed whether these responses provide cross-protection