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    Analysis of non-photochemical quenching in dunaliella salina (green alga)
    (Mahidol University. Mahidol University Library and Knowledge Center, 2023) Rutanachai Thaipratum; Kittisak Yokthongwattana; Jisnuson Svasti
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    Isolation and characterization of a xanthophyll-rich fraction from the thylakoid membrane of Dunaliella salina (green algae)
    (2005-12-01) Kittisak Yokthongwattana; Tatyana Savchenko; Juergen E.W. Polle; Anastasios Melis; University of California, Berkeley; Mahidol University; Brooklyn College
    Long-term acclimation to irradiance stress (HL) of the green alga Dunaliella salina Teod. (UTEX 1644) entails substantial accumulation of zeaxanthin along with a lowering in the relative amount of other pigments, including chlorophylls and several... carotenoids. This phenomenon was investigated with wild type and the zeal mutant of D. salina, grown under conditions of low irradiance (LL), or upon acclimation to irradiance stress (HL). In the wild type, the zeaxanthin to chlorophyll (Zea/Chl) (mol
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    β-carotene production of UV-C induced dunaliella salina under salt stress
    (2019-03-01) Tassnapa Wongsnansilp; Kittisak Yokthongwattana; Sittiruk Roytrakul; Niran Juntawong; Rajamangala University of Technology Krungthep; Kasetsart University; Mahidol University; Thailand National Center for Genetic Engineering and Biotechnology
    © The Author(s) 2019. Dunaliella salina is an important microalga for β-carotene production. In Thailand, the D. salina has not been commercially cultivated. Aim of this study was to screen high β-carotene producing mutants. D. salina KU11 cells
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    Analysis of non-photochemical energy dissipating processes in wild type dunaliella salina (green algae) and in zea1, a mutant constitutively accumulating zeaxanthin
    (2009-07-01) Rutanachai Thaipratum; Anastasios Melis; Jisnuson Svasti; Kittisak Yokthongwattana; Mahidol University; University of California, Berkeley
    excitation energy quenching in the unicellular green alga Dunaliella salina, in both wild type (WT) and a mutant, zea1, constitutively accumulating zeaxanthin under all growth conditions. The results showed that, in D. salina, NPQ is a multi-component process
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    Characterization of fatty acids and proteins associated with the xanthophyll-enriched membrane fraction isolated from the thylakoid membranes of irradiance-stressed Dunaliella salina
    (2010-04-01) Kittisak Yokthongwattana; Malinee Sriariyanun; Pallop Ekaratcharoenchai; Jisnuson Svasti; Mahidol University
    It has been previously reported that a considerable amount of lutein and zeaxanthin could be fractionated, upon mild detergent treatment, from the thylakoid membranes of irradiance-stressed unicellular green alga, Dunaliella salina, into a yellow
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    Efficient transcription of the larvicidal cry4Ba gene from Bacillus thuringiensis in transgenic chloroplasts of the green algal Chlamydomonas reinhardtii
    (2012-08) Thanate Juntadech; Kittisak Yokthongwattana; Sithichoke Tangphatsornruang; Yun-kiam Yap; Gerd Katzenmeier; Chanan Angsuthanasombat; Mahidol University. DivisionInstitute of Molecular Biosciences; Mahidol University. Faculty of Science. Department of Biochemistry and Center for Excellence in Protein Structure and Function
    Unicellular micro-alga Chlamydomonas reinhardtii has been recognized as a promising host for expressing recombinant proteins albeit its limited utility due to low levels of heterologous protein expression. Here, transcription of the 3.4-kb mosquito-larvicidal cry4Ba gene from Bacillus thuringiensis in transgenic C. reinhardtii chloroplasts under control of the promoter and 5’-untranslated region of photosynthetic psbA gene was accomplished. Inverted repeats in chloroplast genomes of the host strain with deleted endogenous psbA genes were selected as recombination targets. Two transformant lines were obtained by dual-phenotypic screening via exhibition of resistance to spectinomycin and restoration of photosynthetic activity. Stable and site-specific integration of intact cry4Ba and psbA genes into chloroplast genomes found in both transgenic lines implied homoplasmy of organelle populations. Achievement in cotranscription of cry4Ba and psbA transgenes revealed by RT-PCR and Northern blot analyses demonstrates the sufficiency of this system’s transcription machinery, offering the further innovation for insecticidal protein production.