15 results
Search Results
Now showing 1 - 10 of 15
Item Metadata only Rapid detection of vibrio parahaemolyticus hemolysin genes in frozen shrimp by using digoxigenin labeled DNA probes(Mahidol University. Mahidol University Library and Knowledge Center, 1997) Pornpich Kowcachaporn; Orasa Suthienkul; Nednapis Tirawanchai; Kanokrat SiripanichgonPublication Metadata only The Forgotten Virulence Factor: The ‘non-conventional’ Hemolysin TlyA And Its Role in Helicobacter pylori Infection(2016-12-01) Mohammad Bagher Javadi; Gerd Katzenmeier; Mahidol Universityof the stomach and disease progression by H. pylori. In a number of earlier studies, TlyA was characterized as a putative pore-forming cytolysin. Although a few observations in the literature suggest a role for TlyA as significant virulence factor of H. pylori..., the molecular and structural characterization of this protein is much curtailed at present. Given the intensive characterization of numerous H. pylori virulence factors over the past decade, surprisingly little information exists for the TlyA toxin and itsPublication Metadata only New facet of non-o1/non-o139 vibrio cholerae hemolysin a: A competitive factor in the ecological niche(2018-01-01) Pichet Ruenchit; Onrapak Reamtong; Kanokrat Siripanichgon; Wanpen Chaicumpa; Pornphan Diraphat; Mahidol University; Faculty of Medicine, Siriraj Hospital, Mahidol Universitypandemic strain. Comparative proteomics of the O1 and the suppressive non-O1/non-O139 strains co-cultured in a simulated natural aquatic microcosm showed that SecB and HlyD were upregulated in the latter. The HlyD-related effective factor was subsequently... found to be hemolysin A (HlyA). However, not all hlyA-positive non-O1/non-O139 strains mediated growth suppression of the N16961 V. cholerae; only strains harboring intact cluster I HlyA could exert this activity. The key feature of the HlyA is locatedItem Metadata only Structural and functional characterization of TlyA hemolysin from Helicobacter pylori(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Nitchakan Samainukul; Katzenmeier, Gerd; Wanpen Chaicumpa; Somphob Leetachewa; Suparerk Borwornpinyo; Chalermpol KanchanawarinHelicobacter pylori gene product HP1086, designated as 'non-conventional' hemolysin named TlyA, is a virulence factor which plays an important role in persistent colonization and disease progression. In this study, 27-kDa TlyA from H. pyloriPublication Metadata only Importance of the Cys124−Cys128 intermolecular disulfide bonding for oligomeric assembly and hemolytic activity of the Helicobacter pylori TlyA hemolysin(2019-06-25) Nitchakan Samainukul; Aung Khine Linn; Mohammad Bagher Javadi; Somsri Sakdee; Chanan Angsuthanasombat; Gerd Katzenmeier; Mahidol University; Faculty of Medicine, Siriraj Hospital, Mahidol University; Chiang Mai University© 2019 Elsevier Inc. Although the TlyA hemolysin from Helicobacter pylori has been implicated as a potential virulence factor involved in mediating host cell colonization and hence disease progression, its structural determinants underlyingPublication Metadata only Contributions of the hydrophobic helix 2 of the Bordetella pertussis CyaA-hemolysin to membrane permeabilization(2018-01-01) Panchika Prangkio; Sirikran Juntapremjit; Melanie Koehler; Peter Hinterdorfer; Chanan Angsuthanasombat; Johannes Kepler Universitat Linz; Mahidol University; Burapha University; Chiang Mai University; Universite Catholique de Louvain; Biophysics Institute for Research and Development (BIRD)© 2018 Bentham Science Publishers. Background: Adenylate cyclase (CyaA) is one of the major virulence factors of Bordetella pertussis that plays a key role in whooping cough pathogenesis. A putative transmembrane helical hairpin (α2-loop-α3...), encompassing residues 529-594 of CyaA hemolysin (CyaA-Hly) domain, was previously proposed to be crucially involved in hemolytic activity against target erythrocytes. Objective: The main objective of this study was to gain more insight into membraneItem Metadata only Meta-Analysis and Systematic Review of Phenotypic and Genotypic Antimicrobial Resistance and Virulence Factors in Vibrio parahaemolyticus Isolated from Shrimp(2024-04-01) Thaotumpitak V.; Odoi J.O.; Anuntawirun S.; Jeamsripong S.; Thaotumpitak V.; Mahidol UniversityThis systematic review and meta-analysis investigates the prevalence of Vibrio parahaemolyticus, its virulence factors, antimicrobial resistance (AMR), and its resistance determinants in shrimp. This study was conducted following the Preferred... Scholar, PubMed, ScienceDirect, and Scopus. The inclusion criteria focused on studies that examined V. parahaemolyticus prevalence, virulence factors, and AMR in shrimp from farms to retail outlets. A total of 32 studies were analyzed, revealing a pooledPublication Metadata only Genetic diversity of Vibrio parahaemolyticus strains isolated from farmed pacific white shrimp and ambient pond water affected by acute hepatopancreatic necrosis disease outbreak in Thailand(2015-12-08) Kaknokrat Chonsin; Shigeaki Matsuda; Chonchanok Theethakaew; Toshio Kodama; Jiraphan Junjhon; Yasuhiko Suzuki; Orasa Suthienkul; Tetsuya Iida; Mahidol University; Osaka University; Hokkaido University; Thammasat Universityand pond water that were positive for AHPND markers AP1 and AP2. PCR analysis confirmed that none of these eight AP-positive AHPND strains possesses the genes for the conventional virulence factors affecting to humans, such as thermostable direct hemolysin... (TDH), TDH-related hemolysin (TRH) and type III secretion system 2. Phylogenetic analysis by multilocus sequence typing showed that the AHPND strains are genetically diverse, suggesting that AHPND strains were not derived from a single genetic lineagePublication Metadata only Identification of differentially expressed proteins from Burkholderia pseudomallei isolated during primary and relapsing melioidosis(2012-04-01) Billie Velapatiño; Direk Limmathurotsakul; Sharon J. Peacock; David P. Speert; The University of British Columbia; Mahidol University; University of Cambridge. Analysis by isotope tagging for relative and absolute quantitation revealed that factors required for nitric oxide detoxification (HmpA) and necessary for anaerobic growth (ArcA, ArcC and ArcB) were highly expressed in the relapse isolate. Two-dimensional... gel electrophoresis revealed up-regulation of a putative hemolysin-coregulated protein in the primary isolate, and flagellin and HSP20/alpha crystalline in the relapse isolate. These observations provide targets for further analysis of latencyPublication Metadata only Alternative Sigma Factor B in Bovine Mastitis-Causing Staphylococcus aureus: Characterization of Its Role in Biofilm Formation, Resistance to Hydrogen Peroxide Stress, Regulon Members(2019-11-07) Sirirak Supa-amornkul; Paninee Mongkolsuk; Pijug Summpunn; Pongkorn Chaiyakunvat; Warisara Navaratdusit; Chutima Jiarpinitnun; Soraya Chaturongakul; Walailak University; Mahidol Universityfactor B (σB), which has been shown to be a global regulator for S. aureus infections, was explored in a mastitis-causing S. aureus strain, RF122. For comparison with the wild-type strain, a sigB null (ΔsigB) mutant was constructed and analyzed for its... or no role in resistance to antiseptics (e.g., povidone-iodine and chlorhexidine), resistance to tested antibiotics, hemolysin activity, and invasion ability. RNA sequencing identified 225 σB-dependent genes, of which 171 are positively regulated and 54