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Item Metadata only An in vitro system for assessing the sensitivity of plasmodium vivax to chloroquine(2002) Oumaporn Tasanor; Noedl, Harald; Kesara Na-Bangchang; Kanungnit Congpuong; Jeeraphat Sirichaisinthop; Wernsdorfer, Walther H; Mahidol University. Faculty of Tropical MedicineFollowing earlier observations on short-term culture of Plasmodium vivax, an in vitro test system has been developed for assessing the parasite's sensitivity to chloroquine. Fresh isolates with predominantly young trophozoites are diluted 1:19... was generally insufficient for an adequate development of the parasites. The reading of the test is based on stage-specific differential counts in the Giemsa-stained pre-incubation and post-incubation thick films, the evaluation on log-probit analysis of drugPublication Metadata only Evaluation of an in vitro test method for the assessment of sensitivity of Plasmodium falciparum to pyrimethamine and sulfadoxine(1987-09-17) A. Sabchareon; T. Chongsuphajaisiddhi; P. Attanath; B. Meemanee; W. Wernsdorfer; Mahidol Universityof sulfadoxine activity. The actions of pyrimethamine in the combination pyrimethamine/sulfadoxine in ratios of 1:80 and 1:200 were similar. In vitro testing using combined pyrimethamine/sulfadoxine should be more precise than pyrimethamine alone for monitoring...Twenty three Plasmodium falciparum isolates collected from two highly pyrimethamine/sulfadoxine-resistant areas of Thailand were evaluated for their in vitro responses to pyrimethamine, sulfadoxine and combinations of these two drugs in variousPublication Metadata only In vitro drug sensitivity testing in Plasmodium vivax(2008-10-01) Walther H. Wernsdorfer; Oumaporn Tasanor; Gunther Wernsdorfer; Medizinische Universitat Wien; Thammasat University; Mahidol UniversityThe possibility of short-term in vitro cultivation, i.e. growth of asexual erythrocytic stages up to the stage of mature schizonts, permits in principle the development of drug sensitivity tests also for Plasmodium vivax. In the absence of a... sequestration of erythrocytes carrying the advanced stages of schizogony, asexual parasites of all stages may be seen in the peripheral blood of patients infected with P. vivax. This precludes schizont maturation tests since schizont development will be undulyPublication Metadata only Is sabin-feldman dye test using T. Gondii tachyzoites from animal inoculation still the best method for detecting toxoplasma gondii antibodies?(2010-09-01) Ruenruetai Udonsom; Ruangrat Buddhirongawatr; Yaowalark Sukthana; Mahidol Universityin vivo and in vitro tachyzoites. We found the conventional dye test detected the highest percent of cases (4.3%), followed by the IFAT using parasites from mice (3.8%), then the dye test and the IFAT using cell culture tachyzoites (both 2.8%). Agreement... with the dye test when using mouse and cell culture derived tachyzoites was 96.7%. Using in vivo tachyzoites for the dye test and the IFAT gave 94.3% agreement, while using in vitro tachyzoites gave 94.8% agreement. When compared with the conventional dye testPublication Metadata only Novel drug compounds against neospora caninum and toxoplasma gondill in vitro.(2007) Jitbanjong Wiengcharoen.; Ryan O'Hanley.; Tanya Armstrong.; Wayne Best.; Yaowalark Sukthana.; RC Andrew Thompson.; Mahidol University. International College. Planning Research and Development Department.Neospora caninum has recently been identified as an important cause of abortion in cattle worldwide. This parasite is closely related to Toxoplasma gondii. To identify the drug compounds for potential use against both parasites in vitro, nine novelPublication Metadata only Is Sabin-Feldman dye test using T. gondii tachyzoites from animal inoculation still the best method for detecting Toxoplasma gondii antibodies?(2010) Ruenruetai Udonsom; Ruangrat Buddhirongawatr; Yaowalark Sukthana; Mahidol University. Mahidol University International College.in vivo and in vitro tachyzoites. We found the conventional dye test detected the highest percent of cases (4.3%), followed by the IFAT using parasites from mice (3.8%), then the dye test and the IFAT using cell culture tachyzoites (both 2.8%). Agreement... with the dye test when using mouse and cell culture derived tachyzoites was 96.7%.Using in vivo tachyzoites for the dye test and the IFAT gave 94.3% agreement,while using in vitro tachyzoites gave 94.8% agreement. When compared with the conventional dye testPublication Metadata only Lymphocyte transformation test and cytokine detection assays: Determination of read out parameters for delayed-type drug hypersensitivity reactions(2021-09-01) Yuttana Srinoulprasert; Siriraj Hospitalthat in vitro tests (if available) be performed prior to any in vivo tests. Therefore, in vitro diagnostic tests can be alternative methods to identify a culprit drug for delayed DHR diagnosis as there is no or very low risk for patients under investigation.... The LTT is the most standardized method whereas it has been available in medical schools affiliated with university hospitals. Other in vitro tests, like cytokine detection assays, have also been used, even though they are still being evaluated. They couldPublication Metadata only Plasmodium vivax malaria in southeast Iran in 1999-2001: Establishing the response to chloroquine in vitro and in vivo(2002-12-01) Y. Hamedi; M. Nateghpour; P. Tan-ariya; M. Tiensuwan; U. Silachamroon; S. Looareesuwan; Tehran University of Medical Sciences; Mahidol Universityhas already been documented in this area. The in vitro sensitivity of 39 P. vivax isolates was assessed: the mean IC50 and IC90 were 189 ng/ml and 698 ng/ml blood respectively; for in vivo testing, all 39 vivax malaria patients were treated with a... standard regimen of chloroquine and followed-up at 28 days: the mean parasite clearance time was 67.2±22.5 hours. The in vitro development of young parasites to mature schizonts in standard test medium was compared with that obtained in McCoy's 5A mediumPublication Metadata only In vivo and in vitro hemostatic activity of Chromolaena odorata leaf extract(2012-09-01) Hataichanok Pandith; Suchitra Thongpraditchote; Yuvadee Wongkrajang; Wandee Gritsanapan; Mahidol University. The hemostatic mechanisms were tested using in vitro platelet aggregation and blood coagulation tests in sheep plasma. Results: All extracts displayed significantly reducing bleeding time ( < 2.5min) in rats but did not induce platelet aggregation or blood... coagulation and platelet aggregation in in vitro tests should be further studied. © 2012 Informa Healthcare USA, Inc.Publication Metadata only In vitro and in vivo antiplasmodial activity and cytotoxicity of water extracts of Phyllanthus emblica, Terminalia chebula, and Terminalia bellerica(2010-12-01) Khosit Pinma; Wanwarang Hiriot; Noppamas Soonthornchareonno; Krisada Jongsaku; Seewaboon Sireeratawon; Siripen Tor-Udo; Thammasat University; Mahidol University; Armed Forces Research Institute of Medical Sciences, Thailandtests were used to detect metabolites in the plant extract. The water extracts of medicinal plants were tested for their antiplasmodial activity in vitro by assessing their ability to inhibit the uptake of [3H] hypoxanthine into the Plasmodium falciparum...Objective: To evaluate the in vitro and in vivo antiplasmodial activity and the cytotoxicity of Phyllanthus emblica Linn, Terminalia chebula Retz, and Terminalia bellerica (Gaertn) Roxb extracts. Material and Method: Standard phytochemical screening