132 results
Search Results
Now showing 1 - 10 of 132
Publication Open Access Platelet inhibition by nitrite is dependent on erythrocytes and deoxygenation(2012) Sirada Srihirun; Thanaporn Sriwantana; Supeenun Unchern; Dusadee Kittikool; Egarit Noulsri; Kovit Pattanapanyasat; Suthat Fucharoen; Barbora Piknova; Alan N. Schechter; Nathawut Sibmooh; Mahidol University. Institute of Science and Technology for Research and Development. Thalassemia Research Center; Mahidol University. Faculty of Science. Department of PharmacologyBackground Nitrite is a nitric oxide (NO) metabolite in tissues and blood, which can be converted to NO under hypoxia to facilitate tissue perfusion. Although nitrite is known to cause vasodilation following its reduction to NO, the effect... of nitrite on platelet activity remains unclear. In this study, the effect of nitrite and nitrite+erythrocytes, with and without deoxygenation, on platelet activity was investigated. Methodology/Finding Platelet aggregation was studied in platelet-richItem Metadata only Mutagenicity of anabolic-androgenic drugs after nitrite treatment and effect of drugs on mutagenicity of aminopyrene (AP)-nitrite model : studies on the formation of direct mutagens during 4H AP-nitrite incubation and on the mutagenic product of 4H AP-nitrite reaction(Mahidol University. Mahidol University Library and Knowledge Center, 2000) Orathai Toobuntherng; Kaew Kangsadalampai; Anadi NitithamyongItem Metadata only Effects of vitamin E on the development of cholangiocarcinoma induced by combined nitrite and aminopyrine(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Pakasit Pratoomtone; Witaya Thamavit; Somnate Boonpucknavig; Galayanee Doungchaweeproducts, rubber industry, leather industry, etc. There are numerous reports showing that cancer can be induced by endogenous nitrosation from the reaction between nitrite and susceptible amine in the stomach. However, endogenous nitrosation can be blocked... by vitamin E which reduces nitrite to nitric oxide and hence make it unavailable for the formation of nitrosamine. Those experiments on inhibitory effect of vitamin E were done in short term. This experiment studied on inhibitory effect of vitamin E in longPublication Metadata only Nitrite in paraffin-stimulated saliva correlates with blood nitrite(2021-11-01) Supanat Lumbikananda; Thanaporn Sriwantana; Krit Rattanawonsakul; Tipparat Parakaw; Chareerut Phruksaniyom; Kanyawat Rattanasuwan; Pornpun Vivithanaporn; Charoensri Thonabulsombat; Nathawut Sibmooh; Sirada Srihirun; Mahidol University, Faculty of Dentistry; Faculty of Medicine Ramathibodi Hospital, Mahidol University; Mahidol UniversityNitrite anion (NO2−) is a circulating nitric oxide (NO) metabolite considered an endothelial function marker. Nitrite can be produced from nitrate (NO3−) secreted from plasma into saliva. The nitrate reductase of oral bacteria converts salivary... nitrate to nitrite, which is swallowed and absorbed into circulation. In this study, we aimed to examine the relevance between these species' salivary and blood levels. We collected three whole saliva samples (unstimulated, paraffin-stimulated, and postPublication Open Access A flow cytometric analysis of the inhibition of platelet reactivity due to nitrite reduction by deoxygenated erythrocytes(2014) Krittapoom Akrawinthawong; Park, Ji Won; Barbora Piknova; Nathawut Sibmooh; Suthat Fucharoen; Schechter, Alan N.; Mahidol University. Faculty of Science. Department of Pharmacology; Mahidol University. Faculty of Medicine Siriraj Hospital. Department of Biochemistry; Mahidol University. Institute of Science and Technology for Research and Development. Thalassemia Research Centerthat the addition of nitrite to platelet-rich plasma in the presence of erythrocytes could inhibit platelet aggregation and this inhibitory effect of nitrite + erythrocytes was enhanced by deoxygenation of erythrocytes as measured by P-selectin expression and c...GMP production. In order to study the nitrite effect on platelets at different oxygen levels, we used the flow cytometric assays to detect platelet membrane surface markers upon activation. The P-selectin and activated gpIIb/IIIa expression on plateletPublication Metadata only Platelet inhibition by nitrite is dependent on erythrocytes and deoxygenation(2012-01-20) Sirada Srihirun; Thanaporn Sriwantana; Supeenun Unchern; Dusadee Kittikool; Egarit Noulsri; Kovit Pattanapanyasat; Suthat Fucharoen; Barbora Piknova; Alan N. Schechter; Nathawut Sibmooh; Mahidol University; The Institute of Science and Technology for Research and Development, Mahidol University; National Institute of Diabetes and Digestive and Kidney DiseasesBackground: Nitrite is a nitric oxide (NO) metabolite in tissues and blood, which can be converted to NO under hypoxia to facilitate tissue perfusion. Although nitrite is known to cause vasodilation following its reduction to NO, the effect... of nitrite on platelet activity remains unclear. In this study, the effect of nitrite and nitrite+erythrocytes, with and without deoxygenation, on platelet activity was investigated. Methodology/Finding: Platelet aggregation was studied in platelet-richItem Metadata only Nitrite scavenging activity of fibers derived from fruits and vegetables, and their antimutagen formation of aminopyrene and nitrite in simulated gastric condition using ames test(Mahidol University. Mahidol University Library and Knowledge Center, 2024) Prapasri Laohavechvanich; Kaew Kangsadalampai; Pongtorn Sungpuag; Wannee KusamranItem Metadata only Innovative dual-mode miniaturized nitrite sensor: Incorporating colorimetric and electrochemical detectors for comprehensive monitoring on a single platform(2024-12-01) Tiawpisitpong P.; Warisson T.; Pinyorospathum C.; Charoenkitamorn K.; Tiawpisitpong P.; Mahidol UniversityThis study introduces a novel, miniaturized analytical platform for a comprehensive range of founded nitrite concentrations on a single, portable device. Nitrites, essential for extending shelf life and enhancing color, are commonly found in both... natural and industrial environments. In environmental settings, it is important to detect low nitrite concentrations, industrial applications, such as in the food industry, require handling higher concentrations effectively. This groundbreaking approachPublication Metadata only Comparison on the Nitrite Scavenging Activity and Antimutagen Formation between Insoluble and Soluble Dietary Fibers(1998-12-01) K. Kangsadalampai; Mahidol UniversityNine insoluble fibers prepared from fruits and vegetables and eleven soluble fibers were evaluated for their nitrite scavenging activity and the antimutagen formation of aminopyrene-nitrite (AP-nitrite) model in the Ames test. Most of the insoluble... fibers were good nitrite scavengers and inhibitors of mutagen which arose from the reaction of the model (pH 3). However, it was found that most of the soluble fibers were not good nitrite scavengers. The presence of some fibers (7 out of 11Publication Metadata only Oxidation of iron-nitrosyl-hemoglobin by dehydroascorbic acid releases nitric oxide to form nitrite in human erythrocytes(2008-03-04) Nathawut Sibmooh; Barbora Piknova; Fabiola Rizzatti; Alan N. Schechter; National Institute of Diabetes and Digestive and Kidney Diseases; Mahidol UniversityThe reaction of deoxyhemoglobin with nitric oxide (NO) or nitrite ions (NO2-) produces ironnitrosyl-hemoglobin (HbNO) in contrast to the reaction with oxyhemoglobin, which produces methemoglobin and nitrate (NO3-). HbNO has not been associated... with the known bioactivities of NO. We hypothesized that HbNO in erythrocytes could be an important source of bioactive NO/nitrite if its oxidation was coupled to the ascorbic acid (ASC) cycle. Studied by absorption and electron paramagnetic resonance (EPR