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Item Metadata only Computational prediction of human immunodeficiency virus type 1 (HIV-1) protease inhibitors resistance using enzyme-ligand docking techniques(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Ekachai Jenwitheesuk; Pranee LeechanachaiItem Metadata only Synthesis, anti HIV-1 protease activity and molecular modeling of non-peptidic chromone derivatives(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Patcharawee Nunthanavanit; Jiraporn UngwitayatornItem Metadata only Qualitative phenotypic resistance prediction from genotypes for human immunodeficiencyency type 1 (HIV-1) protease inhibitors using neural networks(Mahidol University. Mahidol University Library and Knowledge Center, 2004) Ekawat Pasomsub; Wasun ChantratitaItem Metadata only Simultaneous determination of HIV-1 protease inhibitors, lopinavir and retonavir, in syrup by chemometrics-assisted spectrophotometric approach and HPLC method(Mahidol University. Mahidol University Library and Knowledge Center, 2009) Ei, Ei Thin, 1976-; Chutima PhechkrajangPublication Metadata only Design and construction of a synthetic E. coli protease inhibitor detecting biomachine(2017-09-13) P. Boonyalekha; A. Meechai; C. Tayapiwatana; K. Kitidee; D. Waraho-Zhmayev; King Mongkut s University of Technology Thonburi; Chiang Mai University; Mahidol University© 2017 IEEE. Protease inhibitors (PIs) have been used to treat various types of symptoms or diseases. However, current PIs block the protease activity by targeting the protease active site which has been shown to be sensitive to the off-target... effect due to crossreactivity with protease homologues. An alternative approach to inhibiting protease activity is to target the substrate, specifically by blocking the substrate cleavage site. We propose to employ synthetic biology approach to create aItem Metadata only Cloning and characterization of the alkaline protease gene (alpA) from Aspergillus oryzae(Mahidol University. Mahidol University Library and Knowledge Center, 2023) Supaporn Cheevadhanarak; Flegel, Timothy W.; Amaret Bhumiratana; Skorn Mongkolsuk; Holt, GeoffreyThe gene alpA encoding Aspergillllus oryzae alkaline protease (Alp) was isolated from a genomic library of an industrial strain A. oryzae U212 (a high protease producer used in soy sauce production in Thailand) by using oligodeoxyribonucleotide... stability of the transformants was 100% after growth without selective pressure. The alpA gene, introduced into a protease deficient strain (A. oryzae U1638) by cotransformation, directed the secretion of enzymatically active Alp into the culture mediumPublication Metadata only Full length and protease domain activity of chikungunya virus nsP2 differ from other alphavirus nsP2 proteases in recognition of small peptide substrates(2015-01-01) Chonticha Saisawang; Pornpan Sillapee; Kwanhathai Sinsirimongkol; Sukathida Ubol; Duncan R. Smith; Albert J. Ketterman; Mahidol University© 2015 Authors. Alphavirus nsP2 proteins are multifunctional and essential for viral replication. The protease role of nsP2 is critical for virus replication as only the virus protease activity is used for processing of the viral non-structural... polypeptide. Chikungunya virus is an emerging disease problem that is becoming a world-wide health issue. We have generated purified recombinant chikungunya virus nsP2 proteins, both full length and a truncated protease domain from the Cterminus of the nsP2Item Metadata only Synthesis and evaluation of new potent HIV-1 protease inhibitors(Mahidol University. Mahidol University Library and Knowledge Center, 2005) Narumol Phosrithong; Jiraporn UngwitayatornPublication Metadata only An organic solvent-, detergent-, and thermostable alkaline protease from the mesophilic, organic solvent-tolerant Bacillus licheniformis 3C51(2010-10-22) W. Rachadech; A. Navacharoen; W. Ruangsit; T. Pongtharangkul; A. S. Vangnai; Chulalongkorn University; Mahidol UniversityBacillus licheniformis 3C5, isolated as mesophilic bacterium, exhibited tolerance towards a wide range of non-polar and polar organic solvents at 45°C. It produced an extracellular organic solvent-stable protease with an apparent molecular mass... of approximately 32 kDa. The inhibitory effect of PMSF and EDTA suggested it is likely to be an alkaline serine protease. The protease was active over a broad range of temperatures (45-70°C) and pH (8-10) range with an optimum activity at pH 10 and 65°CPublication Open Access Enzymatic analysis of recombinant Japanese encephalitis virus NS2B(H)-NS3pro protease with fluorogenic model peptide substrates(2012-05) Muhammad Junaid; Chakard Chalayut; Anna Sehgelmeble Torrejon; Chanan Angsuthanasombat; Iryna Shutava; Maris Lapins; Jarl E. S. Wikberg; Gerd Katzenmeier; Mahidol University. Institute of Molecular Biosciences. Laboratory of Molecular and Cellular Microbiologyantivirals for JEV would be of great importance, particularly in those cases where the infection has become persistent. Being indispensable for flaviviral replication, the NS2B-NS3 protease is a promising target for design of anti-flaviviral inhibitors.... Contrary to related flaviviral proteases, the JEV NS2B-NS3 protease is structurally and mechanistically much less characterized. Here we aimed at establishing a straightforward procedure for cloning, expression, purification and biochemical characterization